Gba, Gba

Assessment;

Don't use plagiarized sources. Get Your Custom Essay on
Gba, Gba
Just from $13/Page
Order Essay

Analysis

Assessment type;

Treatment Plan

Word limit/length; 2000 words

Overview

Theory is useful as an underpinning to care for consumers, but it’s important to be able to move the theory into action. In the following case study you will have the opportunity to demonstrate that you can take what you have learned and apply it to real-world scenarios

Learning Outcomes

This assessment task is aligned to the following learning outcomes:

1. Appraise a range of complex acute care conditions engagement and interventions

2. Design a package of Trauma Informed Care applicable to acute mental health settings.

Assignment detail ***VERY IMPOTANT FOR YOU TO FOLLOW

Review and analyse the following scenario and create a holistic wellbeing assessment and treatment plan for the individual based on the principles of ‘Trauma Informed Care’. The plan you develop must be supported by critiqued evidenced-based research, with links between physical and mental wellbeing created and justified. Implementation processes of the package/plan must also be designed with specific reference to overcoming inhibiting factors in the consumer’s experience.

The Scenario

Mark was brought into the Emergency Department by a friend who said Mark told him that he was feeling unsafe. His friend advised that he had to leave and didn’t have time to stay. Mark is a 23- year-old Caucasian male. As you greet Mark, you note that he is around 6ft, has dark shoulderlength hair, and has a stocky build with weight held in his abdominal region. Mark presents as unkempt, wearing track pants, torn plain t-shirt and thongs. His feet appear to be unwashed, and his fingernails and toenails are dirty and unmanicured. Mark has limited eye contact and does not respond to your offer to shake his hand. You explain to Mark that your role is to offer support and help him. You further explain that this is done by listening to his concerns and working with him to identify helpful interventions whilst supporting him in keeping safe.

You ask Mark to share some information about himself. Mark explains in a regular rate and softly spoken voice that he has recently separated from his partner Annie and doesn’t know how to live without her. They had been together since he was 17. He explained that they have a four-year-old son together who now lives with Annie. Mark advised that Annie kicked him out of the house because she was sick of him drinking and passing out on the lounge. When she was packing up his stuff, she also found a few bottles of vodka around the house, about which she got upset. He advised that he is now staying with his mates or sleeping in his car. He also reported that he had been picked up by police and charged for high range drink driving a few days ago. Mark states that he hasn’t slept properly in a week.

Mark stated that he works as a labourer doing whatever needs to be done on the job site; however, that job is off and on again. He advised it was the only job he knew and that he didn’t like working out in the heat and that his back had started to hurt. Mark said the only thing he likes about work is going to the pub with the boys for drinks after a day’s work. Even though he knows he shouldn’t, Mark said he sometimes needs a drink before he finishes work to help him get through the day. He doesn’t get as much work now as he did when he was younger and most of his money goes on alcohol and to Annie to help with their son.

His friendship group is limited to the guys he met at the pub and on the worksite. He lost touch with his old school friends when he moved away from home. His parents live in a small rural town, and he doesn’t have anything to do with them. He described his upbringing as really rough and that his father used to drink heavily and would hit his mother when drunk. Mark recalled not having enough money for food or new clothes because his Dad was repeatedly fired from several jobs. He recalled his Dad ended up in the local lock-up for a few days for assaulting a man who confronted him about how he spoke to Mum at the pub. Mark shared how he tried to stop his Dad hitting his Mum but stated that his Dad ended up just hitting him too. Mark believes he was a disappointment to his parents, he feels that his Mum loves him but knows his Dad can’t stand the sight of him and nothing he did was ever good enough. Mark said he missed his Mum but knew he couldn’t go back.

The only thing he liked about growing up in the country was that it taught him how to handle guns. He has a rifle that he hasn’t used in a while as he has no one to go ‘roo shooting’ with.

Mark explained that he hated to leave his Mum but needed to move away from his Dad. He met Annie online when he was in year 11 at school and moved in with her. He didn’t finish school. Mark said that they got along well at the start, and Annie loved him so much. He and Annie did everything together, and then he started work as a labourer. Mark began to drink more, and Annie got upset with this. Mark said that Annie didn’t understand that that’s how he prefers to wind down after a hard day at work. They argued even more, when their son was born. Mark said that everything changed when he came along. He said that Annie told him that he had a problem with drinking and needed to stop.

Mark described his mood as really low, and this is congruent with the way he presented. He added that he felt hopeless and couldn’t do anything right. Mark stated, “I don’t know how I am going to live without Annie; she was my best friend.” Upon assessing the risk of self-harm, Mark stated he had thought about suicide when he was younger, but his uncle talked him out of it. He had a friend who died due to suicide when he was 15, and Mark saw what that did to his family “tore them up”. He further said that he had thought recently about suicide after breaking up with Annie and that the feelings come in waves. He said he had strong feelings again when the police charged him. Mark said he always talked to his uncle about these things as his uncle ‘really understood me’. His uncle passed away from cancer when Mark was 18.

Mark denied having experienced any hallucinations or other altered sensory perceptions. Mark believes he is ‘a loser’ and stated he was afraid he would turn out just like his old man (father). Mark didn’t appear to have any long-term memory impairment; however, he does describe blackouts when he can’t remember how he gets home from the pub. This has been increasing in recent months. He is orientated to place but cannot recall the day, date or time.

You ask Mark what he might need and what might help. His response includes that he hit rock bottom when he got caught drink driving and knows he needs to stop drinking. He would like to get back with Annie and have more time with his son. He also wants to increase his energy – he stated, “look at me, I have no energy, I can’t even be bothered having a shower”. He said his friend brought him here as he told his friend that he couldn’t take much more and wanted to end it all. However, he did add that he doesn’t feel like that all the time and that he currently feels safe talking to you. Mark said he would like a new job and always wanted to work as a mechanic. He also needs somewhere to stay while he works it out with Annie

TASK

*****VERY IMPORTANT TO HELP IN STRUCTURE THIS ASSIGNMENT****

Part A – Approx. 1500 words

Concerning the assessment of Mark provided in the scenario, outline what additional information you would obtain and why to complete a comprehensive and holistic assessment. Ensure you include information for an evaluation of risk.

Part B – Approx. 500 words

Write a letter to Marks GP explaining that you assessed Mark, summarise your findings, what your brief interventions and recommendations were and why, and include any further followup Mark may require from the GP. Keep in mind that Mark will be provided with a copy of the letter, and he should be able to easily read and understand what you consider the problems are and what needs to happen.

Letter Requirements:

1) Outline the critical biological, psychological and social health issues that need to be addressed in this episode of care — paying attention to past trauma/experiences and how they may have contributed to the Marks current presentation.

2) Highlight anything that needs to be addressed to keep Mark safe.

3) Outline your plan of care of brief therapeutic interventions to holistically address/resolve the health problems, referencing the latest evidence and authoritative guidelines.

4) Provide any recommendations that the GP may address to assist Mark in further improving his health, again with reference to the latest evidence.

Assignment Rubric

1-
Criteria; Part A – Additional information & why

Mark 50%

Comprehensive and nuanced outline of the additional information, and why, in order to complete a comprehensive and holistic assessment. Integrates with high quality and relevant evidence and guidelines.

2-
Criteria; Part B – Letter

Mark; 40%;

Comprehensive overview of critical health issues.

Clear and succinct plan of care and recommendations to the GP

3-
Criteria; Writing, grammar and referencing

Mark; 10%

Clear, concise and logically structured paper with a succinct, clear introduction and cogent conclusion.

Very well organised.

Free of grammar and spelling errors.

All citations follow required style.

DEBATE Open Access

Borderline personality disorder and
childhood trauma: exploring the affected
biological systems and mechanisms
Nadia Cattane1, Roberta Rossi2, Mariangela Lanfredi2 and Annamaria Cattaneo1,3,4*

  • Abstract
  • Background
  • : According to several studies, the onset of the Borderline Personality Disorder (BPD) depends on the
    combination between genetic and environmental factors (GxE), in particular between biological vulnerabilities and
    the exposure to traumatic experiences during childhood. We have searched for studies reporting possible
    alterations in several biological processes and brain morphological features in relation to childhood trauma
    experiences and to BPD. We have also looked for epigenetic mechanisms as they could be mediators of the effects
    of childhood trauma in BPD vulnerability.

  • Discussion
  • : We prove the role of alterations in Hypothalamic-Pituitary-Adrenal (HPA) axis, in neurotrasmission, in
    the endogenous opioid system and in neuroplasticity in the childhood trauma-associated vulnerability to develop
    BPD; we also confirm the presence of morphological changes in several BPD brain areas and in particular in those
    involved in stress response.

    Summary: Not so many studies are available on epigenetic changes in BPD patients, although these mechanisms
    are widely investigated in relation to stress-related disorders. A better comprehension of the biological and
    epigenetic mechanisms, affected by childhood trauma and altered in BPD patients, could allow to identify “at high
    risk” subjects and to prevent or minimize the development of the disease later in life.

    Keywords: Borderline personality disorder, Childhood trauma, HPA axis, Endogenous opioid system,
    Neurotransmission, Neuroplasticity, Neuroimaging studies, Epigenetic mechanisms

    Background
    Borderline Personality Disorder (BPD) is a pervasive pat-
    tern of emotional dysregulation, impulsiveness, unstable
    sense of identity and difficult interpersonal relationships
    [1]. The prevalence rates of BPD are between 0.2–1.8%
    in the general community, 15–25% among psychiatric
    inpatients and 10% of all psychiatric outpatients [2, 3].
    Among the different aetiopathological theories that have
    been proposed over years, the most supported is the one
    proposed by Linehan in 1993 [4], which suggests that
    BPD can be the result of the interactions between

    biological and psychosocial factors [2], in particular be-
    tween biologically based temperamental vulnerabilities
    and adverse and traumatic experiences during childhood.
    BPD is a disorder primarily characterized by emotion

    dysregulation and indeed, patients with BPD show
    heightened emotional sensitivity, inability to regulate in-
    tense emotional responses, and a slow return to emo-
    tional baseline. Linehan proposed also that the
    development of BPD occurs within an invalidating devel-
    opmental context characterized by intolerance toward
    the expression of private emotional experiences during
    childhood [4]. As a consequence, children exposed to
    this adverse environment show inability to learn how to
    understand, label, regulate, or tolerate emotional
    responses and, conversely, they vacillate between emo-
    tional inhibition and extreme emotional lability.
    Recently, Hughes and colleagues [5] have proposed an

    integration to the aethiopathogenetic model of BPD,

    * Correspondence: annamaria.cattaneo@kcl.ac.uk;
    acattaneo@fatebenefratelli.eu
    1Biological Psychiatry Unit, IRCCS Istituto Centro San Giovanni di Dio –
    Fatebenefratelli, via Pilastroni 4, Brescia, Italy
    3Stress, Psychiatry and Immunology Laboratory, Department of Psychological
    Medicine, Institute of Psychiatry, King’s College London, 125 Coldharbour
    Lane, London SE5 9NU, UK
    Full list of author information is available at the end of the article

    © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
    International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
    reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
    the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
    (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

    Cattane et al. BMC Psychiatry (2017) 17:221
    DOI 10.1186/s12888-017-1383-2

    http://crossmark.crossref.org/dialog/?doi=10.1186/s12888-017-1383-2&domain=pdf

    mailto:annamaria.cattaneo@kcl.ac.uk

    mailto:acattaneo@fatebenefratelli.eu

    http://creativecommons.org/licenses/by/4.0/

    http://creativecommons.org/publicdomain/zero/1.0/

    emphasizing the role played by a lack of social proximity
    or responsiveness from relevant caregivers in the
    development of BPD symptoms, which in turn impairs
    the individual’s emotion regulation. Affect regulation dif-
    ficulties have been also proposed as key mediators in the
    relationship between childhood trauma and BPD [6].
    Several studies have shown that a diagnosis of BPD is

    associated with child abuse and neglect more than any
    other personality disorders [7, 8], with a range between
    30 and 90% in BPD patients [7, 9].
    Adverse childhood experiences are also related to BPD

    symptom severity [9–11]. In support to this, Widom and
    collaborators [12] followed 500 children who had suf-
    fered physical and sexual abuse and neglect and 396
    matched controls, and they observed that significantly
    more abused/neglected children met criteria for BPD in
    adulthood in comparison to controls. However, the pres-
    ence of a risk factor, such as adverse childhood events,
    was not necessary or sufficient to explain the reason
    why some individuals developed BPD symptoms in
    adulthood, whereas others did not.
    In a recent study, Martin-Blanco and collaborators

    [10] have hypothesized that the interaction of childhood
    trauma and temperamental traits could be associated
    with the severity of BPD. In this regard, they have evalu-
    ated the self-reported history of trauma, the psychobio-
    logical temperamental traits and the severity of the BPD
    symptoms in a cohort of 130 BPD patients. Data showed
    a correlation only between childhood maltreatment and
    sociability and no other correlation was observed. More-
    over, the interaction between high neuroticism-anxiety
    traits and the presence of severe emotional abuse was
    associated with the severity of the disorder.
    Symptom overlap has been reported between BPD

    diagnosis and other disorders including Post-Traumatic
    Stress Disorder (PTSD) and other axis I disorders [13].
    Moreover, in recent decades, different nosographic de-
    scriptions have been suggested to characterize the differ-
    ent symptoms associated with trauma, like complex
    Post-Traumatic Stress Disorder (cPTSD) [14], also
    known as Disorders of Extreme Stress Not Otherwise
    Specified (DESNOS) [15], which describes a clinical syn-
    drome following an experience of interpersonal trau-
    matic victimization and shares many similarities with
    BPD, including pathological dissociation, somatizations,
    dysregulation of emotions, altered central self and rela-
    tional schemas. The definition of cPTSD therefore refers
    to the experience of severe and/or prolonged traumatic
    situations, and does not merely identify the effects of
    devastating traumatic events (like violence or chronic
    maltreatment), which fall under the category of PTSD or
    Acute Stress Disorder. Indeed, exposure to particular
    types of traumatic experiences may result in far more in-
    sidious and crippling psychopathogenic disorders than

    PTSD, compromising the sound development of attach-
    ment behavior related systems and of the ability to
    modulate emotions [16]. Recent research is currently try-
    ing to determine whether cPTSD and BPD diagnosis in
    comorbidity with PTSD are distinct or should both be
    considered and named as trauma-related disorders [17]. A
    recent review [18] has explored the mechanisms through
    which childhood trauma is related to the development of
    BPD in adulthood, and has discussed how interrelated fac-
    tors (such as heritable personality traits, affect regulation
    and dissociation, trauma symptoms) could be mediators
    in the relationship between childhood trauma and BPD.
    Based on all these findings, in the following para-

    graphs we will discuss alterations in several neurobio-
    logical systems and in brain morphology that can be
    induced by exposure to early life adverse experiences
    and that are also associated with BPD (see Table 1). We
    will examine the impact of early stressful events on dif-
    ferent biological systems and mechanisms, possibly iden-
    tifying biomarkers that could be involved in BPD
    vulnerability. This might allow to identify at high risk
    BPD subjects earlier, and to develop intervention strat-
    egies and programs.

    Discussion
    Neurobiological mechanisms involved in BPD
    BPD and the hypothalamic-pituitary-adrenal axis
    The Hypothalamic-Pituitary-Adrenal (HPA) axis is one
    of the neuroendocrine systems which mediate the
    response of the body to stress. Although the stress re-
    sponse mechanism is meant to maintain stability or
    homeostasis, its long-term activation, as consequence of
    chronic stress exposure, may have deleterious effects on
    the body, increasing the risk for developing different
    kinds of illnesses, including stress-related psychiatric
    disorders.
    In stress conditions, corticotropin-releasing factor

    (CRF) and arginine vasopressin (AVP) are released from
    the paraventricular nucleus (PVN) located in the hypo-
    thalamus. These peptides travel through the pituitary
    portal system and act synergistically to stimulate the re-
    lease of the adrenocorticotropic hormone (ACTH) from
    the corticotroph cells. Then, ACTH is transported
    throughout the systemic circulation and binds to recep-
    tors in the adrenal cortex of the adrenal gland, resulting
    in the biosynthesis and release of cortisol [19]. Cortisol
    can affect multiple organs and biological processes, such
    as metabolism, growth, inflammation, cardiovascular
    function, cognition, and behavior [20, 21], by binding to
    specific receptors in the body and in several brain re-
    gions, as the hypothalamus, anterior pituitary and medial
    prefrontal cortex. The central and peripheral effects of
    cortisol are mediated by two intracellular glucocorticoid
    receptor subtypes: the high-affinity type I receptor or

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 2 of 14

    Table 1 Summary of the papers cited in the review and showing alterations in different biological systems in BPD

    Biological systems Authors Sample size Date of study Main Results

    HPA axis Southwick et al. [26] 37 subjects with PTSD comorbid
    with BPD; 18 subjects only with
    PTSD

    2003 Higher 24 h urinary cortisol levels in
    patients with PTSD compared to
    patients with PTSD and comorbid BPD.

    Wingenfeld et al. [27] 21 female patients with BPD; 24
    healthy female controls.

    2007 Higher overnight urinary cortisol levels
    in BPD patients compared to controls.
    Very high cortisol levels were found
    only in BPD patients with a low number
    of PTSD symptoms.

    Rinne et al. [28] 39 BPD patients (24 with and 15
    without sustained childhood
    abuse and comorbid PTSD
    (n = 12) or MDD (n = 11));
    11 control subjects

    2002 Higher ACTH and cortisol levels in the
    blood of BPD females who had
    experienced childhood abuse during
    the DEX/CRH test.

    Carvalho Fernando et al. [29] 32 female BPD patients; 32
    healthy female

    2013 Acute cortisol levels decreased the
    reaction time to target stimuli in both
    BPD patients and controls.

    Martin-Blanco et al. [30] 481 subjects with BPD; 442
    controls

    2016 Case-control study focusing on 47 SNPs
    in 10 HPA axis genes. An association
    between polymorphic variants within
    the FKPB5 and the CRHR genes with
    the diagnosis of BPD was shown. Two
    FKBP5 SNPs were more frequently
    represented in patients with a history
    of childhood trauma.

    Neurotransmission Wagner et al. [42] 159 BPD patients 2009 Association between stressful events
    and low impulsivity in BPD patients.
    5-HTTLPR S-allele carriers showed
    higher impulsivity scores when exposed
    to stressful events than LL omozygotes.

    Wagner et al. [47] 112 female BPD patients 2010 COMT Val158Met SNP was associated
    with early life stressful events and
    impulsive aggression in female BPD
    patients

    Wagner et al. [48] 159 BPD patients 2010 The effect of COMT Val158Met SNP on
    the association between stressful life
    events and impulsivity was not confirmed.

    Tadic et al. [49] 161 Caucasian BPD patients;
    156 healthy controls.

    2009 The COMT Met158Met SNP was
    over-represented in BPD patients compared
    to controls. No differences in 5-HTTLPR
    genotype were found. An interaction
    between the COMT Met158 and the
    5-HTTLPR s-allele was observed.

    Martin-Blanco et al. [50] 481 BPD subjects; 442 controls 2015 Genetic variants within COMT, DBH and
    SLC6A2 genes were associated with an
    enhanced risk to develop BPD

    Endogenous Opioid
    System

    Kalin et al. [57] 8 infant rhesus monkeys
    (4 males and 4 females)

    1988 The endogenous opioid system mediates
    separate-induced vocalizations and
    influences the HPA axis activation in
    rhesus monkeys using the mother-infant
    separation paradigm.

    Prossin et al. [61] 18 un-medicated female BPD
    patients; 14 female controls

    2010 BPD patients had greater regional μ-opioid
    availability at baseline in the left necleus
    accumbens, the hypothalamus and the
    right hippocampus/parahippocampus
    relative to controls, showing an
    endogenous opioid system activation.

    Neuroimaging
    studies

    Driessen et al. [36] 21 female BPD patients;
    21 female controls

    2000 Volume reduction in the hippocampus
    and in the amygdala in BPD patients
    compared to controls.

    Schmahl et al. [38] 25 unmedicated female patients
    with BPD (10 with and 15 without
    comorbid PTSD);
    25 female controls

    2009 Hippocampal volume reduction in
    patients with BPD and comorbid PTSD
    as compared to controls.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 3 of 14

    mineralcorticoid receptor (MR) and the low-affinity type
    receptor or glucocorticoid receptor (GR). It has been
    suggested that MRs have a high affinity for both cortisol
    and aldosterone; they bind cortisol when it is detectable
    at low concentrations. The GRs have a relatively low af-
    finity for cortisol, but high affinity for dexamethasone

    (DEX) [22]; moreover, they bind cortisol at high concen-
    tration, reflecting what occurs in stress conditions.
    The HPA axis is regulated by an auto-regulatory

    mechanism mediated by cortisol itself, that is crucial in
    the maintenance of the homeostatic functions of the
    HPA axis. Indeed, when cortisol levels rise, as in

    Table 1 Summary of the papers cited in the review and showing alterations in different biological systems in BPD (Continued)

    Kreisel et al. [70] 39 BPD patients; 39 controls 2014 Smaller hippocampal volume in BPD
    patients with a lifetime history than
    those without comorbid PTSD.

    Boen et al. [71] 18 women with BPD; 21 controls 2014 Two hippocampal structures (DG-CA4
    and CA2–3 subfields) were significantly
    smaller in patients with BPD than controls.

    Kuhlmann et al. [73] 30 BPD patients; 33 controls 2013 Patients with BPD showed lower
    hippocampal volumes than controls, but
    higher volumes in the hypothalamus.

    Rodrigues et al. [63] 124 BPD patients; 147 controls 2011 Both the left and the right sides of the
    hippocampus were reduced in BPD patients
    with PTSD when compared to controls.

    Ruocco et al. [37] 205 BPD patients; 222 controls 2012 Bilateral volume reductions of the amygdala
    and hippocampus were not related to
    comorbid MDD, PTSD or substance use
    disorders.

    Epigenetics Martin-Blanco et al. [88] 281 subjects with BPD 2014 An association between NR3C1 methylation
    levels and childhood trauma was found in
    blood samples of BPD patients.

    Dammann et al. [89] 26 BPD patients; 11 controls 2011 An increase in the methylation levels of
    HTR2A,NR3C1,MAOA,MAOB and COMT
    was found in BPD patients as compared
    to controls.

    Perroud et al. [91] 346 BD, BPD, and ADHD patients 2016 Differential 5-HT3AR methylation levels
    were associated with the severity of
    childhood trauma, mainly found in BPD
    patients.

    Teschler et al. [93] 24 female BPD patients;
    11 female controls

    2013 Genome-wide methylation analyses revealed
    increased methylation levels of several genes
    (APBA2,APBA3,GATA4,KCNQ1,MCF2,NINJ2,
    TAAR5) in blood of BPD female patients
    and controls.

    Prados et al. [94] 96 BPD subjects suffering from a
    high level of child adversity; 93
    subjects suffering from MDD and
    reporting a low rate of child
    maltreatment

    2015 Several CpGs within or near genes involved
    in inflammation and in neuronal excitability
    were differentially methylated in BPD patients
    compared to MDD patients or in relation to
    the severity of childhood trauma.

    Teschler et al. [95] 24 female BPD patients;
    11 female controls

    2016 A significant aberrant methylation of rDNA and
    PRIMA1 was revealed for BPD patients using
    pyrosequencing. For the promoter of PRIMA1, the
    average methylation of six CpG sites was higher in
    BPD patients compared to controls. In contrast, the
    methylation levels of the rDNA promoter region and
    the 5′ETS were significantly lower in patients with BPD
    compared to controls.

    Neuroplasticity Koenigsberg et al. [109] 24 medication-free BPD patients;
    18 healthy control subjects

    2012 Decrease of PKC and BDNF protein levels in the blood
    of BPD patients.

    Tadic et al. [49] 161 Caucasian BPD patients;
    156 healthy controls.

    2009 Association between HTR1B A-161 variant and the
    functional BDNF 196A allele in BPD patients.

    Perroud et al. [90] 115 subjects with BPD;
    52 controls

    2013 Higher methylation levels in BDNF CpG exons I and IV
    in BPD patients than in controls. Higher BDNF protein
    levels in plasma of BPD patients than in controls.

    Thaler et al. [92] 64 women with bulimia nervosa
    and comorbid BPD; 32 controls

    2014 Hypermethylation within BDNF promoter region sites
    in women with bulimia nervosa and with a history of
    BPD and/or trauma events.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 4 of 14

    response to stress, the MRs are saturated and, conse-
    quently, cortisol binds the GRs, promoting a cascades of
    events that represent the main transduction signals of
    glucocorticoids in stress conditions.
    So far, the HPA axis activity has been widely investi-

    gated in the context of childhood trauma experiences
    and findings support alterations in HPA axis in subjects
    exposed to stress early in life. Indeed, several studies
    have reported alterations in the cortisol circadian
    rhythm and levels, indicating a deregulation of the HPA
    axis responsiveness, due to childhood trauma experi-
    ences, upon stress conditions [23–25].
    Despite the large amount of data on the HPA axis

    functionality as consequence of exposure to stress early
    in life, only a few studies have investigated possible alter-
    ations of this axis in BPD patients. For example, higher
    urinary cortisol levels have been found in BPD patients
    compared to controls [26, 27].
    Southwick and colleagues [26] found higher 24 h urinary

    cortisol levels in patients with PTSD compared to patients
    with PTSD and comorbid BPD, suggesting that these alter-
    ations might reflect differences in the severity of PTSD
    symptoms rather than factors related to BPD per se.
    Another study [27] explored overnight urinary free

    cortisol levels showing higher cortisol levels in BPD pa-
    tients than in controls. A negative association between
    cortisol and PTSD symptoms was also observed. More-
    over, when BPD patients were divided according to the
    presence of high or low number of PTSD symptoms,
    very high cortisol levels were found only in BPD patients
    with a low number of PTSD symptoms. Rinne and col-
    laborators [28] found an exaggerated ACTH and cortisol
    response during the DEX/CRH test in the blood of BPD
    female subjects who had experienced childhood abuse.
    Carvalho Fernando and colleagues [29] investigated the
    effects of cortisol administration on response inhibition
    of emotional stimuli in patients with BPD compared to
    controls. They found that acute cortisol elevations
    decreased the reaction time to target stimuli in both
    BPD patients and controls, but they did not differ in task
    performance.
    Also genetic association studies support alterations in

    HPA axis functionality in association with childhood
    trauma exposure. Martin-Blanco and collaborators [30]
    have investigated the contribution of genetic variants
    within genes in the HPA axis, also in the context of child-
    hood trauma exposures, in a sample of BPD patients and
    controls. The authors performed a case-control study fo-
    cusing on 47 SNPs in 10 HPA axis genes. Data showed an
    association between polymorphic variants within the
    FK506 Binding Protein 5 (FKBP5) and Corticotropin Re-
    leasing Hormone Receptor (CRHR) genes with the diag-
    nosis of BPD. In particular, two FKBP5 polymorphisms,
    rs4713902 and rs9470079, showed significant association

    with BPD. Stronger associations were found in patients
    exposed to childhood trauma where the risk alleles of
    other two FKBP5 polymorphisms, rs3798347-T and
    rs10947563-A, were more frequently represented in
    patients with a history of childhood physical abuse and
    emotional neglect than in patients who had never experi-
    enced these trauma and controls.
    All these findings suggest an association between a

    deregulated functionality of the HPA axis and childhood
    trauma and highlight the involvement of this biological
    system in the development of BPD.

    BPD and neurotransmission
    In addition to the presence of HPA axis dysfunction,
    several studies have also proposed that childhood
    trauma can affect glutamatergic, serotonergic, dopamin-
    ergic and noradrenergic transmission, suggesting that
    BPD is the result of alterations in several interacting
    neurotransmitter systems [31, 32].
    Glutamatergic and N-methyl-D-aspartate (NMDA)

    neurotransmissions play a critical role in neurodevelop-
    ment, synaptic plasticity, learning and memory [33, 34]
    and alterations in all these processes have been involved
    also in the vulnerability and pathophysiology of BPD
    [35]. For example, neuroimaging studies in BPD patients
    as compared to controls have consistently demonstrated
    the presence of decreased synaptic density and volume
    in several brain regions involved in spatial or autobio-
    graphical memory and in the modulation of vigilance
    and negative emotional states, such as hippocampus and
    amygdala, which are also enriched in NMDA receptors
    [36] (see also paragraph “BPD and neuroimaging stud-
    ies”). Moreover, early chronic stress and mistreatments
    experienced during life by BPD patients have been found
    able to impact dendritic arborization, thus contributing
    to the development of morphological alterations associ-
    ated with BPD symptoms [37, 38].
    The serotonin transporter gene (5-HTTLPR) and its

    related signaling in neurotransmission represent another
    system involved in the pathogenesis of BPD [39–42]. In
    particular, a functional single nucleotide polymorphism
    (SNP) within this gene (the 5-HTTLPR S/L SNP) has
    been widely reported to be a modulator of early life
    stressful events by several studies [43–45]; interestingly,
    it has been also associated with BPD symptoms [42, 46].
    For example, Wagner and collaborators [42] investigated
    the effects of 5-HTTLPR S/L SNP and of early life
    stressful events on impulsivity, assessed by the Barratt
    Impulsiveness Scale (BIS), in BPD patients. The authors
    reported an association between the presence of stressful
    events with lower BIS impulsivity scores, suggesting that
    subjects who have experienced trauma, in particular sex-
    ual abuse, may show a reduced impulsivity as a conse-
    quence of the activation of coping mechanisms that

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 5 of 14

    control behavior and social interaction. Further analyses
    conducted by the same authors indicated that S-allele
    carriers showed higher impulsivity scores when exposed
    to early life stressful events as compared to LL omozy-
    gotes, suggesting that patients with 5-HTTLPR S-allele
    are more vulnerable to early life stress. These data high-
    light the contribution of the serotonergic system on im-
    pulsivity in BPD [42].
    Another gene suggested to be a genetic risk factor for

    BPD is represented by Catechol-O-methyltransferase
    (COMT), an enzyme catalyzing the degradation of cate-
    cholamines, including the neurotransmitters dopamine,
    epinephrine, and norepinephrine; however, literature
    data on the role of this gene are contrasting. In a first
    study conducted by Wagner and collaborators [47], the
    COMT Val158Met SNP has been found associated with
    early life stressful events and impulsive aggression,
    assessed by the Buss-Durkee-Hostility Inventory (BDHI)
    sum score, in female BPD patients. In particular, the au-
    thors identified that in COMT Val158Val carriers, but
    not in Val/Met and Met/Met carriers, childhood sexual
    abuse and the cumulative number of stressful events
    were associated with lower BDHI impulsive aggression
    scores. However, in another study conducted by the
    same authors, the effect of the COMT Val158Met SNP
    on the association between stressful life events and im-
    pulsivity was not confirmed [48], probably due to the
    small sample size. The same authors [49] also investi-
    gated, in a group of BPD patients and controls, the role
    of (i) the COMT Val158Met SNP, (ii) the 5-HTTLPR S/L
    variant and (iii) their interaction as genetic vulnerability
    factors for BPD. Data showed that the genotype COMT
    Met158Met was over-represented in BPD patients than
    in controls, whereas no differences in 5-HTTLPR geno-
    type between BPD and controls were reported. In
    addition, the COMT Met158Met genotype was signifi-
    cantly over-represented in BPD patients carrying at least
    one 5-HTTLPR S-allele and, interestingly, an interaction
    between the COMT Met158 and the 5-HTTLPR S-allele
    was also observed. These results suggest an interactive
    effect of COMT and 5-HTTLPR gene variants on the
    vulnerability to develop BPD and, according to the au-
    thors, highlight again the key role of the serotonergic
    and dopaminergic system in the pathogenesis of BPD.
    Martin-Blanco and collaborators [50] investigated the

    possible involvement of the noradrenergic system in BDP
    pathogenesis, by evaluating genetic variants within 4 nor-
    adrenergic genes. In addition to COMT, the authors se-
    lected Dopamine Beta-Hydroxylase (DBH), that acts
    transforming dopamine into noradrenaline, Solute Carrier
    Family 6 Member 2 (SLC6A2), a transporter responsible
    for the reuptake of extracellular neurotransmitters, and
    Adrenoceptor Beta 2 (ADRB2), that mediates the
    catecholamine-induced activation of adenylate cyclase

    through the action of G proteins. The authors’ findings in-
    dicated that only genetic variants within 3 genes (COMT,
    DBH and SLC6A2) were associated with an enhanced risk
    to develop BPD.
    These studies, taken together, show that alterations in

    several neurotransmitter systems could be involved in
    BPD pathogenesis; however, due to the small number of
    available studies, further investigations are needed.

    BPD and the endogenous opioid system
    According to Bandelow and Schmahl’s theory, a reduc-
    tion in the sensitivity of the opioid receptors or in the
    availability of endogenous opioids might constitute part
    of the underlying pathophysiology of BPD [51].
    Endogenous opioids mainly include three classes (en-

    dorphins, enkephalins and dynorphins), which activate
    three types of G protein-coupled receptors (μ, δ, and κ
    opioid receptors [52]). One of the most important en-
    dogenous opioid is β-endorphin which is synthesized in
    part in the arcuate nucleus of the hypothalamus and is
    released into the blood, the spinal cord and in various
    brain regions, including reward-related areas [53]. β-
    endorphin is activated by a variety of stressors [54] and
    induce euphoria and analgesic effects (for example dur-
    ing childbirth and during positive experiences [55]).
    The μ-opioid receptors appear to be more relevant for

    the social and affective regulation associated with BPD,
    suggesting that this system can contribute to the interper-
    sonal vulnerabilities and intrapersonal pain of BPD. These
    receptors are widely distributed throughout the human
    Central Nervous System (CNS), with a particular density
    in the basal ganglia, cortical structures, thalamic nuclei,
    spinal cord, and specific nuclei in the brainstem [56].
    The endogenous opioid system modulates responses

    to acute and chronic stressful and noxious stimuli that
    induce physical, emotional, or social pain. In animal
    models, the endogenous opioid system has been
    implicated in affiliative responses, emotion and stress
    regulation, including stress-induced analgesia and
    impulsive-like behavior [57]. Using the mother-infant
    separation paradigm in rhesus monkeys, Kalin and col-
    laborators [57] studied for the first time the role of the
    opioid system in modulating the behavioural and neuro-
    endocrine consequences of a brief occurring stressor.
    The authors conducted several experiments where ani-
    mals received morphine, an opioid agonist, naloxone, an
    opioid antagonist or both to test the increase in
    vocalization and the activation of the HPA axis in infant
    primates separated or not from their mothers. The re-
    sults showed that morphine significantly decreased
    separation-induced vocalizations and locomotion with-
    out affecting activity levels, whereas naloxone increased
    separation-induced vocalizations and environmental ex-
    ploration. When the two drugs were co-administered,

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 6 of 14

    the effect of morphine was reversed only with the
    0.1 mg/kg dose of naloxone. The authors also assessed
    the effects of separation on neuroendocrine function
    and tested whether activation of the opioid system may
    attenuate these effects by measuring plasma concentra-
    tions of ACTH and cortisol in infant rhesus monkeys
    separated or not separated from their mothers, treated
    with morphine or naloxone or co-treated with the two
    drugs. Plasma ACTH and cortisol levels were higher in
    infant rhesus monkeys separated from their mothers
    compared to not separated ones, confirming the involve-
    ment of the HPA axis during stress exposure. However,
    only ACTH plasma levels were modulated by morphine
    and by naloxone and by their interaction in the group of
    infant separated by their mothers. These findings suggest
    that the endogenous opioid system is involved in medi-
    ating separation-induced vocalizations and influences
    the HPA axis activation following a stress condition.
    In humans, regional endogenous opioid system activa-

    tion has been associated with suppression of both sen-
    sory and affective qualities of stressors and with trait
    impulsivity [58–60] whereas its regional deactivation has
    been related to hyperalgesic responses and increases in
    negative affect during stress [61]. The hypothesis is that
    the activation of the μ-opioid receptors could have a
    suppressive effect during emotional or physical chal-
    lenges that threaten organism homeostasis.
    Research has described regional alterations in the

    function of the endogenous opioid system and μ-opioid
    receptors in brain regions involved in emotion and stress
    processing, decision making, and pain and neuroendo-
    crine regulation. However, to date, there is only limited
    evidence of alterations of endogenous opioid levels in
    BPD patients. In an interesting study Prossin and collab-
    orators [61] investigated the role of the endogenous opi-
    oid system and μ-opioid receptors in emotion regulation
    in un-medicated female BPD patients compared to fe-
    male controls by using positron emission tomography
    (PET) (see paragraph “BPD and neuroimaging studies”
    for details).
    Comparing BPD patients to their matched controls,

    the authors found significant differences in baseline re-
    gional μ-opioid receptor concentrations in vivo, as well
    as in this neurotransmitter system’s response to a nega-
    tive emotional challenge that can be related to some of
    the clinical characteristics of BPD.

    BPD and neuroimaging studies
    Volumetric alterations in brain areas involved in stress
    response
    To date, several functional and structural in vivo neuro-
    imaging studies have been performed in BPD patients,
    detecting alterations mainly localized in the limbic cir-
    cuit and in frontal cortex. These regions are related to

    the distinctive clinical features of the disorder (i.e impul-
    sivity, aggression, and emotional reactivity). The most
    replicated result, confirmed in recent meta-analyses [37,
    62, 63], is represented by the reduction in the volumes
    of the hippocampus and the amygdala of BPD patients
    compared to controls [36, 64–69]. The robustness of this
    finding seems to suggest that volumetric decreases in
    these two brain areas could be specific for BPD and thus
    useful as possible endophenotypes of illness. In 2000
    Driessen and collaborators [36] performed the first mag-
    netic resonance imaging volumetric measurement of the
    hippocampus, amygdala, temporal lobes, and prosen-
    cephalon in 21 female BPD patients and female controls,
    reporting in BPD patients a volume reduction of the
    16% in the hippocampus and of the 8% in the amygdala.
    Moreover, hippocampal volumes were negatively corre-
    lated with the extent and the duration of self-reported
    early trauma, but only in the entire sample of BPD pa-
    tients and controls.
    The role of PTSD and trauma as comorbidity with BPD

    on hippocampus and amygdala volumes has been object
    of investigation but the results are still controversial.
    Schmahl and colleagues [38] compared two groups of un-
    medicated BPD female patients with and without comor-
    bid PTSD and 25 female controls. They found reduced
    hippocampal volumes only in patients with BPD and co-
    morbid PTSD but not in BPD patients without a history
    of PTSD as compared to controls. Similarly, Kreisel and
    collaborators [70] investigated in details the hippocampal
    structural volumes comparing 39 BPD patients with 39
    matched controls, and, although no volume differences
    were found between the two groups, patients with a life-
    time history of PTSD had a smaller hippocampal volume
    (−10,5%) than those without comorbid PTSD. Boen and
    collaborators [71] investigated the volumes of the Cornu
    Ammonis (CA) and the Dentate Gyrus (DG), two hippo-
    campal structures prone to morphological changes [72] in
    response to adverse environmental changes in a group of
    18 women with BPD and 21 controls. The authors found
    that the stress-vulnerable DG-CA4 and CA2–3 subfields
    were significantly smaller in patients with BPD than in
    controls. However, they did not identify any significant
    association between subfield volumes and reported child-
    hood trauma.
    In another interesting study, Kuhlmann and collabora-

    tors [73] investigated alterations in the grey matter of
    central stress-regulating structures, including hippocam-
    pus, amygdala, anterior cingulate cortex and hypothal-
    amus, in female patients with BPD and controls. The
    authors also explored whether grey matter volume of
    these four brain structures was associated with child-
    hood trauma, reporting that patients with BPD showed
    lower hippocampal volumes than healthy controls, but
    higher volumes in the hypothalamus. Interestingly,

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 7 of 14

    hypothalamic volume correlated positively with a history
    of trauma in patients with BPD.
    Two recent meta-analyses [37, 63] evaluated whether

    the magnitude of hippocampus and amygdala volume
    reductions may be associated with state-of-illness factors
    and psychiatric disorders (i.e. PTSD) which often co-
    occured with BPD. In the Rodrigues’ meta-analysis, the
    authors included 7 articles with a total number of 124
    patients and 147 controls. They showed that both the
    left and the right sides of hippocampal volumes were re-
    duced in BPD patients with PTSD when compared to
    controls. The left hippocampal volume was not signifi-
    cantly smaller in BPD patients without PTSD relative to
    healthy controls and the right hippocampal volume was
    reduced in patients with BPD without comorbid PTSD,
    but to a lesser degree than in BPD patients with PTSD.
    In contrast, the results reported by Ruocco’s meta-
    analysis [37] which included 11 studies with a total num-
    ber of 205 BPD patients and 222 controls, revealed that
    bilateral volume reductions of the amygdala and hippo-
    campus were unrelated to comorbid Major Depressive
    Disorder (MDD), PTSD, or substance use disorders.
    Taken together, all these studies show that the main

    brain regions involved in BPD are those associated to
    stress and highlight the importance of classifying sub-
    groups of patients with BPD, especially taking into ac-
    count the presence of comorbidity with PTSD or of a
    history of childhood trauma. Notwithstanding, the asso-
    ciation between the volume reduction and the degree to
    which childhood trauma could be responsible for these
    changes remains unclear.

    Endogenous opiod system alterations in brain regions
    involved in stress response
    Despite a large amount of data referred to volumetric
    and morphological alterations in brain regions associated
    to specific clinical features of BPD, not many neuroim-
    aging studies have been conducted to investigate the role
    of the endogenous opioid system in BPD. As previously
    mentioned, Prossin and collaborators [61] measured the
    in vivo availability of the μ-opioid receptors (non-dis-
    placeable binding potential (BPND)) in a group of un-
    medicated female BPD patients compared to female con-
    trols by using PET and the selective radiotracer [11C]
    carfentanil at baseline and during sustained sadness
    states. Patients had greater regional μ-opioid BPND than
    controls at baseline (neutral state) in the left nucleus ac-
    cumbens, the hypothalamus, and the right hippocam-
    pus/parahippocampus relative to comparison subjects,
    showing an endogenous opioid system activation. As
    suggested by the authors, differences between BPD pa-
    tients and controls in baseline in vivo μ-opioid receptor
    concentrations and in the endogenous opioid system re-
    sponse to a negative emotional challenge can be related

    to some of the clinical characteristics of BPD patients.
    These findings show alterations in the function of the
    endogenous opioid system and μ-opioid receptors in
    brain regions involved in emotion and stress processing,
    decision making, and pain and neuroendocrine regula-
    tion, features also associated with BPD.

    BPD and epigenetic mechanisms
    The influence of environmental factors, such as child-
    hood trauma, has been suggested to occur through
    epigenetic mechanisms, which may underlie gene-
    environment associated vulnerability to develop stress-
    related disorders [74] including BPD where childhood
    trauma history occurs in most of the patients (with a
    range between 30 and 90%) [7, 9].
    Among the most investigated epigenetic mechanisms

    there are: (i) DNA methylation, which occurs at CG
    dinucleotides (CpG) and can influence the spatial struc-
    ture of the DNA and the binding or the repression of
    specific DNA-binding proteins to the DNA [75], (ii) his-
    tone modifications, which influence the condensation of
    the DNA around histone proteins and regulate the ac-
    cessibility of functional regions to transcriptional factors
    [76] and (iii) post-transcriptional regulation by non-
    coding RNAs such as microRNAs (miRNAs) [77].
    All these epigenetic processes and, in particular,

    changes in DNA methylation have been widely investi-
    gated in the context of long-term negative effects of
    early life stressful events. In non-human primates and in
    rodents, several paradigms of stress early in life, includ-
    ing maternal separation or prenatal stress have been as-
    sociated with epigenetic alterations via DNA
    methylation [78, 79]. For example, non-stressed dams
    during pregnancy showed increased frequency of licking
    and grooming in the first week of the puppies’ life that
    were associated with changes in DNA methylation
    within the promoter of genes, such as glucocorticoid re-
    ceptor gene (NR3C1), known to be involved in behavior
    and neurodevelopment.
    The hypothesis is that the quality of maternal care, af-

    fected by stress or depression in pregnancy and post-
    partum [80, 81] could impact, through epigenetic mech-
    anisms, on gene expression and behavioral traits that are
    maintained throughout life [78].
    Recently, McGowan and colleagues [79] examined

    DNA methylation, histone acetylation and gene expres-
    sion in a 7 million base pair region of chromosome 18
    containing the NR3C1 gene in the hippocampus of adult
    rat offspring, whose mothers differed in the frequency of
    maternal care. The authors found that the adult off-
    spring of high compared to low maternal care showed a
    pattern of regions spanning the NR3C1 gene which were
    differentially methylated and acetylated, highlighting the
    idea that epigenetic changes, in the context of early life

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 8 of 14

    stress, involve alterations in gene-networks rather than
    in a single or few genes.
    Similarly, studies in humans reported similar results as

    those found in rodents, including the increased methyla-
    tion levels within the NR3C1 promoter region in sub-
    jects who reported a history of early life adverse events
    [82–84]. For example, in another interesting study,
    McGowan and collaborators [82] found that in humans
    the cytosine methylation levels of the NR3C1 promoter
    were significantly increased in the postmortem hippo-
    campus obtained from suicide victims with a history of
    childhood abuse as compared with those from suicide
    victims with no childhood abuse or with control sam-
    ples. Decreased levels of NR3C1 mRNA were also identi-
    fied, suggesting an effect of childhood abuse on NR3C1
    methylation status and gene expression, independently
    from suicide.
    Several epigenetic studies have been also conducted in

    control subjects characterized for a history of childhood
    trauma compared to those with no childhood trauma. In
    this context, Suderman and colleagues [85] have demon-
    strated, by using a genome-wide promoter DNA methy-
    lation approach, an abuse-associated hypermethylation
    in 31 miRNAs in a sample of control adult males
    exposed to childhood abuse. The hypermethylated state
    for 6 of these miRNAs was consistent with an hypome-
    thylation status of their target genes.
    Reduced methylation levels of FKBP5 gene within

    regions containing functional glucocorticoid responsive
    elements (GRE) were also found in the blood of control
    individuals exposed to childhood abuse when compared
    to subjects without a history of trauma [86]. This de-
    methylation was linked to increased stress-dependent
    gene transcription followed by a long-term dysregulation
    of the stress hormone system and a global effect on the
    function of immune cells and brain areas associated with
    stress regulation. Thus, according to the authors, the
    changes in FKBP5 methylation levels might increase the
    differential responsiveness of FKBP5 to GR activation
    that can remain stable over time. Moreover, Labontè and
    colleagues [87] have conducted a genome-wide study of
    promoter methylation in the hippocampus of individuals
    with a history of severe childhood abuse and control
    subjects. Methylation profiles were then compared with
    corresponding genome-wide gene expression profiles.
    Among all the differentially methylated promoters, 248
    showed hypermethylation whereas 114 demonstrated hy-
    pomethylation and genes involved in cellular/neuronal
    plasticity were among the most significantly differentially
    methylated.
    Despite the contribution of DNA methylation has been

    extensively investigated in association with childhood
    trauma in the context of pathologies related to stress,
    studies on the possible involvement of epigenetic

    mechanisms in BPD vulnerability are only at their birth.
    Indeed, only few studies are available. In particular,
    Martin-Blanco and colleagues, investigated the associ-
    ation between NR3C1 methylation status, history of
    childhood trauma and clinical severity in blood samples
    of BPD subjects, showing an association between
    NR3C1 methylation and childhood trauma, in the form
    of physical abuse, and a trend towards significance for
    emotional neglect [88]. Regarding NR3C1 methylation
    and clinical severity, the authors also found a significant
    association with self injurious behavior and previous
    hospitalizations. All these findings support the hypoth-
    esis that alterations in NR3C1 methylation can occur
    early in life as consequence of stress exposure and can
    persist up to adulthood where subjects with higher
    NR3C1 methylation levels are also those with enhanced
    vulnerability to develop BPD.
    Above to DNA methylation changes within NR3C1,

    hypo- or hyper-methylation within other genes have
    been found to play a key role in mediating the impact of
    early life stress on the development of stress-related dis-
    orders, including BPD [89–92]. For example, in a study
    conducted by Dammann and colleagues [89] DNA
    methylation pattern of 14 genes, selected because previ-
    ously associated with BPD and other psychiatric disor-
    ders, (COMT, Dopamine Transporter 1 (DAT1),
    Gamma-Aminobutyric Acid Type A Receptor Alpha1
    Subunit (GABRA1), G Protein Subunit Beta 3 (GNB3),
    Glutamate Ionotropic Receptor NMDA Type Subunit 2B
    (GRIN2B), 5-Hydroxytryptamine Receptor 1B (HTR1B),
    5-Hydroxytryptamine Receptor 2A (HTR2A), Serotonin
    Transporter 1 (5-HTT), Monoamine Oxidase A
    (MAOA), Monoamine Oxidase B (MAOB), Nitric Oxide
    Synthase 1 (NOS1), NR3C1, Tryptophan Hydroxylase 1
    (TPH1) and Tyrosine Hydroxylase (TH)), was analyzed
    in the whole blood of BPD patients and controls. An in-
    crease in the methylation levels of HTR2A, NR3C1,
    MAOA, MAOB and COMT was observed in BPD
    patients as compared to controls, suggesting that an in-
    creased methylation of CpG sites within these genes
    may contribute to BPD aetiopathogenesis. Recently,
    Perroud and colleagues [91] investigated the role of
    childhood trauma on the methylation status of the
    Serotonin 3A Receptor (5-HT3AR), including several
    CpGs located within or upstream this gene. They ana-
    lyzed its association with clinical severity outcomes, also
    in relation with a functional genetic SNP (rs1062613)
    within 5-HT3AR in adult patients with Bipolar Disorder,
    BPD, and Attention Deficit Hyperactivity Disorder
    (ADHD). The results showed that differential 5-HT3AR
    methylation status was dependent on the history of
    childhood maltreatment and the clinical severity of the
    psychiatric disorder; this association was not specifically
    restricted to one specific psychiatric disorders

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 9 of 14

    investigated by the authors, but was found in patients
    who reported the higher severity indexes of childhood
    maltreatment, mainly represented by BPD patients. In
    particular, childhood physical abuse was associated with
    higher 5-HT3AR methylation levels, whereas childhood
    emotional neglect was inversely correlated with CpG1 I
    methylation levels. As suggested by the authors, these
    results highlight the need to search for history of child-
    hood maltreatment in patients suffering from psychiatric
    disorders as these events could be associated with the
    worse negative outcomes. Moreover, the authors found a
    modulation of the 5HT3AR methylation status by
    rs1062613 at CpG2 III, where patients carrying the risk
    CC genotype showed the highest levels of methylation at
    CpG2 III. Since C allele has been also associated with a
    lower expression levels of 5HT3AR, the authors sug-
    gested that increased methylation, due to exposure to
    childhood maltreatment, could lead to a further decrease
    in the expression of 5HT3AR mRNA.
    Aiming to identify novel genes that may exhibit aber-

    rant DNA methylation frequencies in BPD patients,
    Teschler and collaborators [93] performed a genome-
    wide methylation analysis in the blood of BPD female
    patients and female controls. The authors reported in-
    creased methylation levels of several genes, including
    neuronal adaptor proteins (Amyloid Beta Precursor
    Protein Binding Family A Member 2 (APBA2) and
    Amyloid Beta Precursor Protein Binding Family A Mem-
    ber 3 (APBA3)), zinc-finger transcription factors (GATA
    Binding Protein 4 (GATA4)), voltage-gated potassium
    channel gene (Potassium Voltage-Gated Channel Sub-
    family Q Member 1 (KCNQ1)), guanine nucleotide ex-
    change factors (Proto-Oncogene MCF-2 (MCF2)),
    adhesion molecules (Ninjurin 2 (NINJ2)) and G protein-
    coupled receptors (Trace Amine Associated Receptor 5
    (TAAR5)) in BPD samples compared to controls. Simi-
    larly, using a whole-genome methylation approach, Pra-
    dos and colleagues [94] analyzed the global DNA
    methylation status in the peripheral blood leukocytes of
    BPD patients with a history of childhood adversity and
    also in patients with MDD characterized by a low rate of
    childhood maltreatment. Contrary to Teschler [93], who
    used control subjects as reference group, in this study
    the authors used MDD subjects, most of them suicide
    attempters, thus controlling not only for MDD but also
    for a history of suicide. The authors also assessed pos-
    sible correlations between methylation signatures and
    the severity of childhood maltreatment. Data showed
    that several CpGs within or near genes involved in in-
    flammatory processes (Interleukin 17 Receptor A
    (IL17RA)), regulation of gene expression (miR124–3)
    and neuronal excitability and development/maintenance
    of the nervous system (Potassium Voltage-Gated Chan-
    nel Subfamily Q Member 2 (KCNQ2)) were differentially

    methylated, either in BPD compared with MDD or in re-
    lation to the severity of childhood maltreatment.
    In a more recent study, Teschler and collaborators

    [95] have analyzed also DNA methylation patterns of the
    ribosomal RNA gene (rDNA promoter region and 5′-ex-
    ternal transcribed spacer/5′ETS) and the promoter of
    the proline rich membrane anchor 1 gene (PRIMA1) in
    peripheral blood samples of female BPD patients and
    controls. The authors have identified a significant aber-
    rant methylation of rDNA and PRIMA1 in the group of
    BPD patients. Specifically, the average methylation of 6
    CpG sites in the promoter of PRIMA1 was 1.6-fold
    higher in BPD patients compared to controls. In con-
    trast, the methylation levels of the rDNA promoter re-
    gion and the 5′ETS were significantly lower (0.9-fold) in
    patients with BPD compared to controls. Furthermore,
    decreased methylation levels were found for nine CpGs
    located in the rDNA promoter region and for 4 CpGs at
    the 5′ETS in peripheral blood of patients compared to
    controls. These results suggest that aberrant methylation
    of rDNA and PRIMA1 could be associated with the
    pathogenesis of BPD.
    Taken together, all these studies reveal a complex

    interplay between BPD, early-life stressful adversities and
    epigenetic signatures.

    BPD and neuroplasticity (the role of BDNF)
    Neuroplasticity refers to brain-related mechanisms
    associated with the ability of the brain to perceive, adapt
    and respond to a variety of internal and external stimuli
    [96, 97], including stress.
    The exposure to acute stressful challenges can induce

    several beneficial and protective effects for the body,
    which responds to almost any sudden, unexpected events
    by releasing chemical mediators – i.e. catecholamines that
    increase heart rate and blood pressure – and help the in-
    dividual to cope with the situation [20, 98–101]. However,
    a chronic exposure to stress and thus a chronic exposure
    to glucocorticoids can have negative and persistent effects
    on the body, including altered metabolism, altered im-
    munity, enhanced inflammation, cognitive deficits, and
    also an enhanced vulnerability for psychiatric disorders
    and for medical conditions such as cardiovascular disease,
    metabolic disorders and cancer [102, 103].
    Neurotrophic factors, and in particular the neurotro-

    phin Brain-Derived Neurotrophic Factor (BDNF), have
    been identified as key mediators of stress on neuronal
    connectivity, dendritic arborization, synaptic plasticity
    and neurogenesis [104–107]. Since its crucial role in
    brain development and brain plasticity, BDNF has been
    widely investigated also in several psychiatric diseases,
    including BPD [108].
    For example, Koenigsberg and colleagues [109] found

    a decrease of Protein Kinase C (PKC) isoenzyme, which

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 10 of 14

    is a molecule downstream the activation of BDNF, and
    BDNF protein levels in the blood of BPD patients, sug-
    gesting an alteration of BDNF signaling and conse-
    quently of neuroplasticity-related mechanisms in BPD.
    In another study, Tadic and collaborators [49] investi-
    gated the association between BPD and genetic variants
    within HTR1B and BDNF genes. Although data showed
    no significant differences in genotype or haplotype dis-
    tribution for both HTR1B and BDNF variants between
    BPD patients and controls, logistic regression analyses
    revealed an association between the HTR1B A-161 vari-
    ant and the functional BDNF 196A allele in BPD.
    Importantly, several findings have also documented epi-

    genetic modifications on BDNF gene in patients with
    BPD, suggesting that childhood maltreatment in BPD pa-
    tients can cause long term epigenetic alterations of genes
    crucially involved in brain functions and neurodevelop-
    ment, including BDNF, and that these alterations may
    contribute to enhanced vulnerability to develop BPD path-
    ology. In this regard, Perroud and collaborators [90] mea-
    sured the percentage of methylation at BDNF CpG exons
    I and IV and also plasma BDNF protein levels in subjects
    with BPD and controls. The authors reported significantly
    higher methylation status in both CpG regions in patients
    than in controls, with the number of childhood trauma
    exposures associated with the high levels of BDNF methy-
    lation. Moreover, BPD patients had significantly higher
    BDNF plasma protein levels than controls, but this in-
    crease was not associated with changes in BDNF methyla-
    tion status. More recently, Thaler and collaborators [92]
    analyzed DNA methylation patterns in the promoter re-
    gion of BDNF gene in women with bulimia nervosa and
    with history of BPD and/or trauma events. They reported
    that bulimia nervosa was associated per se with an hyper-
    methylation within BDNF promoter region sites. This was
    particularly evident when co-occurring with childhood
    abuse or BPD.
    Overall, these studies support the hypothesis that child-

    hood trauma could be associated with changes in BDNF
    epigenetic signature, that in turn could contribute to alter
    cognitive functions in BPD patients. Indeed, higher levels
    of gene methylation are commonly accompanied by a re-
    duced gene expression. Thus higher BDNF methylation
    levels should determine reduced expression of BDNF gene
    and reduced BDNF mRNA levels are widely observed in
    patients with psychiatric diseases [110–112].

  • Conclusions
  • Up to now, neither a specific gene variant or biological
    mechanism has been exclusively associated with BPD,
    but the onset of this disorder has been suggested to
    depend on the combination of a vulnerable genetic back-
    ground with adverse environmental factors during
    childhood.

    Among the biological systems found involved in BPD
    pathogenesis and particularly affected by childhood
    trauma events, there are: the HPA axis, the neurotrans-
    mission mechanisms, the endogenous opioid system and
    the neuroplasticity. In line with the involvement of these
    processes, neuroimaging studies in BPD patients have
    shown volume reductions in the hippocampus and amyg-
    dala, both brain regions mainly involved in stress
    responses, cognition, memory and emotion regulation and
    an increase in the μ-opioid receptors in the same areas.
    Among the environmental factors, early life stressful

    events, in particular childhood trauma, have been pro-
    posed to negatively impact brain development through
    epigenetic mechanisms. Although a complex interplay
    between BPD, early-life stressful adversities and epigen-
    etic signatures has been suggested, further investigations
    are needed in order to better understand the role of gen-
    etic background and traumatic events during childhood
    in the onset of BPD. A better comprehension of these
    interactions could allow to identify at risk subjects, who
    could be treated with preventive therapies, such as psy-
    chotherapy, and to prevent or minimize the develop-
    ment of the disease later in life.

  • Abbreviations
  • 5-HT3AR: Serotonin 3A Receptor; 5-HTT: Serotonin Transporter 1; 5-HTTLPR: Serotonin
    transporter gene; ACTH: Adrenocorticotropic Hormone; ADHD: Attention Deficit
    Hyperactivity Disorder; ADRB2: Adrenoceptor Beta 2; APBA2: Amyloid Beta Precursor
    Protein Binding Family A Member 2; APBA3: Amyloid Beta Precursor Protein Binding
    Family A Member 3; AVP: Arginine Vasopressin; BDHI: Buss-Durkee-Hostility Inventory;
    BDNF: Brain-Derived Neurotrophic Factor; BIS: Barratt Impulsiveness Scale;
    BPD: Borderline Personality Disorder; CA: Cornu Ammonis; CNS: Central Nervous
    System; COMT: Catechol-O-methyltransferase; CpG: CG dinucleotides;
    cPTSD: complex Post-Traumatic Stress Disorder; CRF: Corticotropin-Releasing Factor;
    CRHR: Corticotropin Releasing Hormone Receptor; DAT1: Dopamine Transporter 1;
    DBH: Dopamine Beta-Hydroxylase; DESNOS: Disorders of Extreme Stress Not
    Otherwise Specified; DEX: Dexamethasone; DG: Dentate Gyrus; FKBP5: FK506 Binding
    Protein 5; GABRA1: Gamma-Aminobutyric Acid Type A Receptor Alpha1 Subunit;
    GATA4: GATA Binding Protein 4; GNB3: G Protein Subunit Beta 3; GR: Glucocorticoid
    Receptor; GRE: Glucocorticoid Responsive Elements; GRIN2B: Glutamate Ionotropic
    Receptor NMDA Type Subunit 2B; HPA axis: Hypothalamic-Pituitary-Adrenal axis;
    HTR1B: 5-Hydroxytryptamine Receptor 1B; HTR2A: 5-Hydroxytryptamine Receptor 2A;
    IL17RA: Interleukin 17 Receptor A; KCNQ1: Potassium Voltage-Gated Channel Sub-
    family Q Member 1; KCNQ2: Potassium Voltage-Gated Channel Subfamily Q Member
    2; MAOA: Monoamine Oxidase A; MAOB: Monoamine Oxidase B; MCF2: Proto-
    Oncogene MCF-2; MDD: Major Depressive Disorder; miRNAs: microRNAs;
    MR: Mineralcorticoid Receptor; NINJ2: Ninjurin 2; NMDA: N-methyl-D-aspartate;
    NOS1: Nitric Oxide Synthase 1; NR3C1: Glucocorticoid receptor gene; PET: Positron
    Emission Tomography; PKC: Protein Kinase C; PRIMA1: Prolin Rich Membrane Anchor
    1; PTSD: Post-Traumatic Stress Disorder; PVN: Paraventricular Nucleus; SLC6A2: Solute
    Carrier Family 6 Member 2; SNP: Single nucleotide polymorphism; TAAR5: Trace
    Amine Associated Receptor 5; TH: Tyrosine Hydroxylase; TPH1: Tryptophan
    Hydroxylase 1

  • Acknowledgements
  • Not applicable.

  • Funding
  • This work was supported by an Eranet-Neuron Grant to A.C. (Inflame-D
    project) and by funding from the Italian Ministry of Health (MoH) to A.C.

  • Availability of data and materials
  • The data supporting the conclusions of this article are included within the
    article.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 11 of 14

  • Authors’ contributions
  • N.C. managed the literature searches and wrote the first draft of the manuscript.
    R.R. and M.L. managed the literature searches and completed the manuscript.
    A.C. revised and approved the final version of the manuscript. All authors gave
    their scientific contribution and have approved the final manuscript.

  • Competing interests
  • All the authors declare that they have no conflicts of interest.
    All the authors certify that the submission is an original work and it is not
    under review at any other journal.

  • Consent for publication
  • Not applicable- as the submitted manuscript is a review.

  • Ethics approval and consent to participate
  • Not applicable- as the submitted manuscript is a review.

  • Author details
  • 1Biological Psychiatry Unit, IRCCS Istituto Centro San Giovanni di Dio –
    Fatebenefratelli, via Pilastroni 4, Brescia, Italy. 2Psychiatry Unit, IRCCS Istituto
    Centro San Giovanni di Dio – Fatebenefratelli, via Pilastroni 4, Brescia, Italy.
    3Stress, Psychiatry and Immunology Laboratory, Department of Psychological
    Medicine, Institute of Psychiatry, King’s College London, 125 Coldharbour
    Lane, London SE5 9NU, UK. 4Department of Psychological Medicine, Institute
    of Psychiatry, Psychology and Neuroscience, King’s College London, 125
    Coldharbour Lane, London SE5 9NU, UK.

    Received: 7 February 2017 Accepted: 6 June 2017

  • References
  • 1. Regier DA, Kuhl EA, Kupfer DJ. The DSM-5: classification and criteria

    changes. World psychiatry : official journal of the World Psychiatric
    Association. 2013;12(2):92–8. doi:10.1002/wps.20050.

    2. Leichsenring F, Leibing E, Kruse J, New AS, Leweke F. Borderline personality
    disorder. Lancet. 2011;377(9759):74–84. doi:10.1016/S0140-6736(10)61422-5.

    3. Lieb K, Zanarini MC, Schmahl C, Linehan MM, Bohus M. Borderline personality
    disorder. Lancet. 2004;364(9432):453–61. doi:10.1016/S0140-6736(04)16770-6.

    4. Linehan MM. Dialectical behavior therapy for treatment of borderline
    personality disorder: implications for the treatment of substance abuse.
    NIDA Res Monogr. 1993;137:201–16.

    5. Hughes AE, Crowell SE, Uyeji L, Coan JA. A developmental neuroscience of
    borderline pathology: emotion dysregulation and social baseline theory. J
    Abnorm Child Psychol. 2012;40(1):21–33. doi:10.1007/s10802-011-9555-x.

    6. van Dijke A, Ford JD, van der Hart O, van Son M, van der Heijden P, Buhring
    M. Affect dysregulation in borderline personality disorder and somatoform
    disorder: differentiating under- and over-regulation. J Personal Disord. 2010;
    24(3):296–311. doi:10.1521/pedi.2010.24.3.296.

    7. Battle CL, Shea MT, Johnson DM, Yen S, Zlotnick C, Zanarini MC, et al.
    Childhood maltreatment associated with adult personality disorders:
    findings from the collaborative longitudinal personality disorders study. J
    Personal Disord. 2004;18(2):193–211.

    8. Yen S, Shea MT, Battle CL, Johnson DM, Zlotnick C, Dolan-Sewell R, et al.
    Traumatic exposure and posttraumatic stress disorder in borderline,
    schizotypal, avoidant, and obsessive-compulsive personality disorders:
    findings from the collaborative longitudinal personality disorders study. J
    Nerv Ment Dis. 2002;190(8):510–8. doi:10.1097/01.NMD.0000026620.66764.78.

    9. Zanarini MC, Frankenburg FR, Hennen J, Reich DB, Silk KR. Prediction of the
    10-year course of borderline personality disorder. Am J Psychiatry. 2006;
    163(5):827–32. doi:10.1176/ajp.2006.163.5.827.

    10. Martin-Blanco A, Soler J, Villalta L, Feliu-Soler A, Elices M, Perez V, et al.
    Exploring the interaction between childhood maltreatment and
    temperamental traits on the severity of borderline personality disorder.
    Compr Psychiatry. 2014;55(2):311–8. doi:10.1016/j.comppsych.2013.08.026.

    11. Gunderson JG, Weinberg I, Daversa MT, Kueppenbender KD, Zanarini MC,
    Shea MT, et al. Descriptive and longitudinal observations on the relationship
    of borderline personality disorder and bipolar disorder. Am J Psychiatry.
    2006;163(7):1173–8. doi:10.1176/appi.ajp.163.7.1173.

    12. Widom CS, Czaja SJ, Paris J. A prospective investigation of borderline
    personality disorder in abused and neglected children followed up into

    adulthood. J Personal Disord. 2009;23(5):433–46. doi:10.1521/pedi.2009.23.5.
    433.

    13. Pagura J, Stein MB, Bolton JM, Cox BJ, Grant B, Sareen J. Comorbidity of
    borderline personality disorder and posttraumatic stress disorder in the U.S.
    population. J Psychiatr Res. 2010;44(16):1190–8. doi:10.1016/j.jpsychires.2010.
    04.016.

    14. Herman JL. Complex PTSD: A syndrome in survivors of prolonged and
    repeated trauma. J Trauma Stress 1992;5(3):377–391. doi:10.1002/jts.
    2490050305.

    15. Luxenberg T, Spinazzola, J., Hidalgo, J., Hunt, C., Van Der Kolk, B.A. Complex
    trauma and disorders of extreme stress (DESNOS) diagnosis, Part One:
    Assessment Directions in Psychiatry 2001;21:373–393.

    16. D’Andrea W, Ford J, Stolbach B, Spinazzola J, van der Kolk BA.
    Understanding interpersonal trauma in children: why we need a
    developmentally appropriate trauma diagnosis. The American journal of
    orthopsychiatry. 2012;82(2):187–200. doi:10.1111/j.1939-0025.2012.01154.x.

    17. Cloitre M, Garvert DW, Weiss B, Carlson EB, Bryant RA. Distinguishing PTSD,
    Complex PTSD, and borderline personality disorder: a latent class analysis.
    Eur J Psychotraumatol 2014;5. doi:10.3402/ejpt.v5.25097.

    18. MacIntosh HG, N.; Dubash, N.;. Borderline personality disorder: disorder of
    trauma or personality, a review of the empirical literature. Can Psychol
    2015;56:227–241.

    19. Pompili M, Serafini G, Innamorati M, Moller-Leimkuhler AM, Giupponi G,
    Girardi P, et al. The hypothalamic-pituitary-adrenal axis and serotonin
    abnormalities: a selective overview for the implications of suicide
    prevention. Eur Arch Psychiatry Clin Neurosci. 2010;260(8):583–600. doi:10.
    1007/s00406-010-0108-z.

    20. Lupien SJ, Maheu F, Tu M, Fiocco A, Schramek TE. The effects of stress and
    stress hormones on human cognition: implications for the field of brain and
    cognition. Brain Cogn. 2007;65(3):209–37. doi:10.1016/j.bandc.2007.02.007.

    21. Harris BN, Carr JA. The role of the hypothalamus-pituitary-adrenal/interrenal
    axis in mediating predator-avoidance trade-offs. General and comparative
    endocrinology. 2016;230–231:110–42. doi:10.1016/j.ygcen.2016.04.006.

    22. De Kloet ER. Why dexamethasone poorly penetrates in brain. Stress.
    1997;2(1):13–20.

    23. Carpenter LL, Carvalho JP, Tyrka AR, Wier LM, Mello AF, Mello MF, et al.
    Decreased adrenocorticotropic hormone and cortisol responses to stress in
    healthy adults reporting significant childhood maltreatment. Biol Psychiatry.
    2007;62(10):1080–7. doi:10.1016/j.biopsych.2007.05.002.

    24. Maniam J, Antoniadis C, Morris MJ. Early-life stress, HPA Axis adaptation, and
    mechanisms contributing to later health outcomes. Front Endocrinol.
    2014;5:73. doi:10.3389/fendo.2014.00073.

    25. Papadopoulos AS, Cleare AJ. Hypothalamic-pituitary-adrenal axis dysfunction
    in chronic fatigue syndrome. Nat Rev Endocrinol. 2012;8(1):22–32. doi:10.
    1038/nrendo.2011.153.

    26. Southwick SM, Axelrod SR, Wang S, Yehuda R, Morgan CA 3rd, Charney D,
    et al. Twenty-four-hour urine cortisol in combat veterans with PTSD and
    comorbid borderline personality disorder. J Nerv Ment Dis. 2003;191(4):261–
    2. doi:10.1097/01.NMD.0000061140.93952.28.

    27. Wingenfeld K, Driessen M, Adam B, Hill A. Overnight urinary cortisol release
    in women with borderline personality disorder depends on comorbid PTSD
    and depressive psychopathology. European psychiatry : the journal of the
    Association of European Psychiatrists. 2007;22(5):309–12. doi:10.1016/j.
    eurpsy.2006.09.002.

    28. Rinne T, de Kloet ER, Wouters L, Goekoop JG, DeRijk RH, van den Brink W.
    Hyperresponsiveness of hypothalamic-pituitary-adrenal axis to combined
    dexamethasone/corticotropin-releasing hormone challenge in female
    borderline personality disorder subjects with a history of sustained
    childhood abuse. Biol Psychiatry. 2002;52(11):1102–12.

    29. Carvalho Fernando S, Beblo T, Schlosser N, Terfehr K, Wolf OT, Otte C, et al.
    Acute glucocorticoid effects on response inhibition in borderline personality
    disorder. Psychoneuroendocrinology. 2013;38(11):2780–8. doi:10.1016/j.
    psyneuen.2013.07.008.

    30. Martin-Blanco A, Ferrer M, Soler J, Arranz MJ, Vega D, Calvo N, et al. The role
    of hypothalamus-pituitary-adrenal genes and childhood trauma in
    borderline personality disorder. Eur Arch Psychiatry Clin Neurosci.
    2016;266(4):307–16. doi:10.1007/s00406-015-0612-2.

    31. Friedel RO. Dopamine dysfunction in borderline personality disorder: a
    hypothesis. Neuropsychopharmacology : official publication of the American
    College of Neuropsychopharmacology. 2004;29(6):1029–39. doi:10.1038/sj.
    npp.1300424.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 12 of 14

    http://dx.doi.org/10.1002/wps.20050

    http://dx.doi.org/10.1016/S0140-6736(10)61422-5

    http://dx.doi.org/10.1016/S0140-6736(04)16770-6

    http://dx.doi.org/10.1007/s10802-011-9555-x

    http://dx.doi.org/10.1521/pedi.2010.24.3.296

    http://dx.doi.org/10.1097/01.NMD.0000026620.66764.78

    http://dx.doi.org/10.1176/ajp.2006.163.5.827

    http://dx.doi.org/10.1016/j.comppsych.2013.08.026

    http://dx.doi.org/10.1176/appi.ajp.163.7.1173

    http://dx.doi.org/10.1521/pedi.2009.23.5.433

    http://dx.doi.org/10.1521/pedi.2009.23.5.433

    http://dx.doi.org/10.1016/j.jpsychires.2010.04.016

    http://dx.doi.org/10.1016/j.jpsychires.2010.04.016

    http://dx.doi.org/10.1002/jts.2490050305

    http://dx.doi.org/10.1002/jts.2490050305

    http://dx.doi.org/10.1111/j.1939-0025.2012.01154.x

    http://dx.doi.org/10.3402/ejpt.v5.25097

    http://dx.doi.org/10.1007/s00406-010-0108-z

    http://dx.doi.org/10.1007/s00406-010-0108-z

    http://dx.doi.org/10.1016/j.bandc.2007.02.007

    http://dx.doi.org/10.1016/j.ygcen.2016.04.006

    http://dx.doi.org/10.1016/j.biopsych.2007.05.002

    http://dx.doi.org/10.3389/fendo.2014.00073

    http://dx.doi.org/10.1038/nrendo.2011.153

    http://dx.doi.org/10.1038/nrendo.2011.153

    http://dx.doi.org/10.1097/01.NMD.0000061140.93952.28

    http://dx.doi.org/10.1016/j.eurpsy.2006.09.002

    http://dx.doi.org/10.1016/j.eurpsy.2006.09.002

    http://dx.doi.org/10.1016/j.psyneuen.2013.07.008

    http://dx.doi.org/10.1016/j.psyneuen.2013.07.008

    http://dx.doi.org/10.1007/s00406-015-0612-2

    http://dx.doi.org/10.1038/sj.npp.1300424

    http://dx.doi.org/10.1038/sj.npp.1300424

    32. Figueroa E, Silk KR. Biological implications of childhood sexual abuse in
    borderline personality disorder. J Personal Disord. 1997;11(1):71–92.

    33. Snyder MA, Gao WJ. NMDA hypofunction as a convergence point for
    progression and symptoms of schizophrenia. Front Cell Neurosci. 2013;7:31.
    doi:10.3389/fncel.2013.00031.

    34. Kahn RS, Sommer IE. The neurobiology and treatment of first-episode
    schizophrenia. Mol Psychiatry. 2015;20(1):84–97. doi:10.1038/mp.2014.66.

    35. Grosjean B, Tsai GE. NMDA neurotransmission as a critical mediator of
    borderline personality disorder. Journal of psychiatry & neuroscience : JPN.
    2007;32(2):103–15.

    36. Driessen M, Herrmann J, Stahl K, Zwaan M, Meier S, Hill A, et al. Magnetic
    resonance imaging volumes of the hippocampus and the amygdala in
    women with borderline personality disorder and early traumatization. Arch
    Gen Psychiatry. 2000;57(12):1115–22.

    37. Ruocco AC, Amirthavasagam S, Zakzanis KK. Amygdala and hippocampal
    volume reductions as candidate endophenotypes for borderline personality
    disorder: a meta-analysis of magnetic resonance imaging studies. Psychiatry
    Res. 2012;201(3):245–52. doi:10.1016/j.pscychresns.2012.02.012.

    38. Schmahl C, Berne K, Krause A, Kleindienst N, Valerius G, Vermetten E, et al.
    Hippocampus and amygdala volumes in patients with borderline
    personality disorder with or without posttraumatic stress disorder. Journal
    of psychiatry & neuroscience : JPN. 2009;34(4):289–95.

    39. Ni X, Sicard T, Bulgin N, Bismil R, Chan K, McMain S, et al. Monoamine
    oxidase a gene is associated with borderline personality disorder. Psychiatr
    Genet. 2007;17(3):153–7. doi:10.1097/YPG.0b013e328016831c.

    40. Pascual JC, Soler J, Barrachina J, Campins MJ, Alvarez E, Perez V, et al. Failure
    to detect an association between the serotonin transporter gene and
    borderline personality disorder. J Psychiatr Res. 2008;42(1):87–8. doi:10.1016/
    j.jpsychires.2006.10.005.

    41. Tadic A, Baskaya O, Victor A, Lieb K, Hoppner W, Dahmen N. Association
    analysis of SCN9A gene variants with borderline personality disorder. J
    Psychiatr Res. 2008;43(2):155–63. doi:10.1016/j.jpsychires.2008.03.006.

    42. Wagner S, Baskaya O, Lieb K, Dahmen N, Tadic A. The 5-HTTLPR
    polymorphism modulates the association of serious life events (SLE) and
    impulsivity in patients with borderline personality disorder. J Psychiatr Res.
    2009;43(13):1067–72. doi:10.1016/j.jpsychires.2009.03.004.

    43. Harkness KL, Bagby RM, Stewart JG, Larocque CL, Mazurka R, Strauss JS, et al.
    Childhood emotional and sexual maltreatment moderate the relation of the
    serotonin transporter gene to stress generation. J Abnorm Psychol. 2015;
    124(2):275–87. doi:10.1037/abn0000034.

    44. Benedetti F, Riccaboni R, Poletti S, Radaelli D, Locatelli C, Lorenzi C, et al.
    The serotonin transporter genotype modulates the relationship between
    early stress and adult suicidality in bipolar disorder. Bipolar Disord. 2014;
    16(8):857–66. doi:10.1111/bdi.12250.

    45. Duman EA, Canli T. Influence of life stress, 5-HTTLPR genotype, and SLC6A4
    methylation on gene expression and stress response in healthy Caucasian males.
    Biology of mood & anxiety disorders. 2015;5:2. doi:10.1186/s13587-015-0017-x.

    46. Paaver M, Nordquist N, Parik J, Harro M, Oreland L, Harro J. Platelet MAO
    activity and the 5-HTT gene promoter polymorphism are associated with
    impulsivity and cognitive style in visual information processing.
    Psychopharmacology. 2007;194(4):545–54. doi:10.1007/s00213-007-0867-z.

    47. Wagner S, Baskaya O, Anicker NJ, Dahmen N, Lieb K, Tadic A. The catechol
    o-methyltransferase (COMT) val(158)met polymorphism modulates the
    association of serious life events (SLE) and impulsive aggression in female
    patients with borderline personality disorder (BPD). Acta Psychiatr Scand.
    2010;122(2):110–7. doi:10.1111/j.1600-0447.2009.01501.x.

    48. Wagner S, Baskaya O, Lieb K, Dahmen N, Tadic A. Lack of modulating effects
    of the COMT Val(158)met polymorphism on the association of serious life
    events (SLE) and impulsivity in patients with borderline personality disorder.
    J Psychiatr Res. 2010;44(2):121–2. doi:10.1016/j.jpsychires.2009.06.008.

    49. Tadic A, Elsasser A, Victor A, von Cube R, Baskaya O, Wagner S, et al.
    Association analysis of serotonin receptor 1B (HTR1B) and brain-derived
    neurotrophic factor gene polymorphisms in borderline personality disorder.
    J Neural Transm. 2009;116(9):1185–8. doi:10.1007/s00702-009-0264-3.

    50. Martin-Blanco A, Ferrer M, Soler J, Arranz MJ, Vega D, Bauza J, et al. An
    exploratory association study of the influence of noradrenergic genes and
    childhood trauma in borderline personality disorder. Psychiatry Res. 2015;
    229(1–2):589–92. doi:10.1016/j.psychres.2015.07.046.

    51. Bandelow B, Schmahl C, Falkai P, Wedekind D. Borderline personality
    disorder: a dysregulation of the endogenous opioid system? Psychol Rev.
    2010;117(2):623–36. doi:10.1037/a0018095.

    52. Feng Y, He X, Yang Y, Chao D, Lazarus LH, Xia Y. Current research on opioid
    receptor function. Curr Drug Targets. 2012;13(2):230–46.

    53. Dikshtein Y, Barnea R, Kronfeld N, Lax E, Roth-Deri I, Friedman A, et al. Beta-
    endorphin via the delta opioid receptor is a major factor in the incubation
    of cocaine craving. Neuropsychopharmacology : official publication of the
    American College of Neuropsychopharmacology. 2013;38(12):2508–14. doi:
    10.1038/npp.2013.155.

    54. Roth-Deri I, Green-Sadan T, Yadid G. Beta-endorphin and drug-induced
    reward and reinforcement. Prog Neurobiol. 2008;86(1):1–21. doi:10.1016/j.
    pneurobio.2008.06.003.

    55. Esch T, Stefano GB. The neurobiology of Love. Neuro endocrinology letters.
    2005;26(3):175–92.

    56. Stanley B, Siever LJ. The interpersonal dimension of borderline personality
    disorder: toward a neuropeptide model. Am J Psychiatry. 2010;167(1):24–39.
    doi:10.1176/appi.ajp.2009.09050744.

    57. Kalin NH, Shelton SE, Barksdale CM. Opiate modulation of separation-
    induced distress in non-human primates. Brain Res. 1988;440(2):285–92.

    58. Zubieta JK, Ketter TA, Bueller JA, Xu Y, Kilbourn MR, Young EA, et al.
    Regulation of human affective responses by anterior cingulate and limbic
    mu-opioid neurotransmission. Arch Gen Psychiatry. 2003;60(11):1145–53.
    doi:10.1001/archpsyc.60.11.1145.

    59. Zubieta JK, Smith YR, Bueller JA, Xu Y, Kilbourn MR, Jewett DM, et al.
    Regional mu opioid receptor regulation of sensory and affective dimensions
    of pain. Science. 2001;293(5528):311–5. doi:10.1126/science.1060952.

    60. Love TM, Stohler CS, Zubieta JK. Positron emission tomography measures of
    endogenous opioid neurotransmission and impulsiveness traits in humans. Arch
    Gen Psychiatry. 2009;66(10):1124–34. doi:10.1001/archgenpsychiatry.2009.134.

    61. Prossin AR, Love TM, Koeppe RA, Zubieta JK, Silk KR. Dysregulation of
    regional endogenous opioid function in borderline personality disorder. Am
    J Psychiatry. 2010;167(8):925–33. doi:10.1176/appi.ajp.2010.09091348.

    62. Nunes PM, Wenzel A, Borges KT, Porto CR, Caminha RM, de Oliveira IR.
    Volumes of the hippocampus and amygdala in patients with borderline
    personality disorder: a meta-analysis. J Personal Disord. 2009;23(4):333–45.
    doi:10.1521/pedi.2009.23.4.333.

    63. Rodrigues E, Wenzel A, Ribeiro MP, Quarantini LC, Miranda-Scippa A, de
    Sena EP, et al. Hippocampal volume in borderline personality disorder with
    and without comorbid posttraumatic stress disorder: a meta-analysis.
    European psychiatry : the journal of the Association of European
    Psychiatrists. 2011;26(7):452–6. doi:10.1016/j.eurpsy.2010.07.005.

    64. Irle E, Lange C, Sachsse U. Reduced size and abnormal asymmetry of
    parietal cortex in women with borderline personality disorder. Biol
    Psychiatry. 2005;57(2):173–82. doi:10.1016/j.biopsych.2004.10.004.

    65. Brambilla P, Soloff PH, Sala M, Nicoletti MA, Keshavan MS, Soares JC.
    Anatomical MRI study of borderline personality disorder patients. Psychiatry
    Res. 2004;131(2):125–33. doi:10.1016/j.pscychresns.2004.04.003.

    66. Tebartz van Elst L, Hesslinger B, Thiel T, Geiger E, Haegele K, Lemieux L,
    et al. Frontolimbic brain abnormalities in patients with borderline
    personality disorder: a volumetric magnetic resonance imaging study. Biol
    Psychiatry. 2003;54(2):163–71.

    67. Rossi R, Lanfredi M, Pievani M, Boccardi M, Beneduce R, Rillosi L, et al.
    Volumetric and topographic differences in hippocampal subdivisions in
    borderline personality and bipolar disorders. Psychiatry Res. 2012;203(2–3):
    132–8. doi:10.1016/j.pscychresns.2011.12.004.

    68. Rossi R, Pievani M, Lorenzi M, Boccardi M, Beneduce R, Bignotti S, et al.
    Structural brain features of borderline personality and bipolar disorders.
    Psychiatry Res. 2013;213(2):83–91. doi:10.1016/j.pscychresns.2012.07.002.

    69. O’Neill A, D’Souza A, Carballedo A, Joseph S, Kerskens C, Frodl T. Magnetic
    resonance imaging in patients with borderline personality disorder: a study
    of volumetric abnormalities. Psychiatry Res. 2013;213(1):1–10. doi:10.1016/j.
    pscychresns.2013.02.006.

    70. Kreisel SH, Labudda K, Kurlandchikov O, Beblo T, Mertens M, Thomas C, et al.
    Volume of hippocampal substructures in borderline personality disorder.
    Psychiatry Res. 2015;231(3):218–26. doi:10.1016/j.pscychresns.2014.11.010.

    71. Boen E, Westlye LT, Elvsashagen T, Hummelen B, Hol PK, Boye B, et al.
    Smaller stress-sensitive hippocampal subfields in women with borderline
    personality disorder without posttraumatic stress disorder. Journal of
    psychiatry & neuroscience : JPN. 2014;39(2):127–34.

    72. Teicher MH, Anderson CM, Polcari A. Childhood maltreatment is associated
    with reduced volume in the hippocampal subfields CA3, dentate gyrus, and
    subiculum. Proc Natl Acad Sci U S A. 2012;109(9):E563–72. doi:10.1073/pnas.
    1115396109.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 13 of 14

    http://dx.doi.org/10.3389/fncel.2013.00031

    http://dx.doi.org/10.1038/mp.2014.66

    http://dx.doi.org/10.1016/j.pscychresns.2012.02.012

    http://dx.doi.org/10.1097/YPG.0b013e328016831c

    http://dx.doi.org/10.1016/j.jpsychires.2006.10.005

    http://dx.doi.org/10.1016/j.jpsychires.2006.10.005

    http://dx.doi.org/10.1016/j.jpsychires.2008.03.006

    http://dx.doi.org/10.1016/j.jpsychires.2009.03.004

    http://dx.doi.org/10.1037/abn0000034

    http://dx.doi.org/10.1111/bdi.12250

    http://dx.doi.org/10.1186/s13587-015-0017-x

    http://dx.doi.org/10.1007/s00213-007-0867-z

    http://dx.doi.org/10.1111/j.1600-0447.2009.01501.x

    http://dx.doi.org/10.1016/j.jpsychires.2009.06.008

    http://dx.doi.org/10.1007/s00702-009-0264-3

    http://dx.doi.org/10.1016/j.psychres.2015.07.046

    http://dx.doi.org/10.1037/a0018095

    http://dx.doi.org/10.1038/npp.2013.155

    http://dx.doi.org/10.1016/j.pneurobio.2008.06.003

    http://dx.doi.org/10.1016/j.pneurobio.2008.06.003

    http://dx.doi.org/10.1176/appi.ajp.2009.09050744

    http://dx.doi.org/10.1001/archpsyc.60.11.1145

    http://dx.doi.org/10.1126/science.1060952

    http://dx.doi.org/10.1001/archgenpsychiatry.2009.134

    http://dx.doi.org/10.1176/appi.ajp.2010.09091348

    http://dx.doi.org/10.1521/pedi.2009.23.4.333

    http://dx.doi.org/10.1016/j.eurpsy.2010.07.005

    http://dx.doi.org/10.1016/j.biopsych.2004.10.004

    http://dx.doi.org/10.1016/j.pscychresns.2004.04.003

    http://dx.doi.org/10.1016/j.pscychresns.2011.12.004

    http://dx.doi.org/10.1016/j.pscychresns.2012.07.002

    http://dx.doi.org/10.1016/j.pscychresns.2013.02.006

    http://dx.doi.org/10.1016/j.pscychresns.2013.02.006

    http://dx.doi.org/10.1016/j.pscychresns.2014.11.010

    http://dx.doi.org/10.1073/pnas.1115396109

    http://dx.doi.org/10.1073/pnas.1115396109

    73. Kuhlmann A, Bertsch K, Schmidinger I, Thomann PA, Herpertz SC.
    Morphometric differences in central stress-regulating structures between
    women with and without borderline personality disorder. Journal of
    psychiatry & neuroscience : JPN. 2013;38(2):129–37. doi:10.1503/jpn.120039.

    74. Klengel T, Binder EB. Epigenetics of stress-related psychiatric disorders and
    Gene x environment interactions. Neuron. 2015;86(6):1343–57. doi:10.1016/j.
    neuron.2015.05.036.

    75. Slatkin M. Epigenetic inheritance and the missing heritability problem.
    Genetics. 2009;182(3):845–50. doi:10.1534/genetics.109.102798.

    76. Levine A, Worrell TR, Zimnisky R, Schmauss C. Early life stress triggers
    sustained changes in histone deacetylase expression and histone H4
    modifications that alter responsiveness to adolescent antidepressant
    treatment. Neurobiol Dis. 2012;45(1):488–98. doi:10.1016/j.nbd.2011.09.005.

    77. Issler O, Chen A. Determining the role of microRNAs in psychiatric disorders.
    Nat Rev Neurosci. 2015;16(4):201–12. doi:10.1038/nrn3879.

    78. Kaffman A, Meaney MJ. Neurodevelopmental sequelae of postnatal
    maternal care in rodents: clinical and research implications of molecular
    insights. Journal of child psychology and psychiatry, and allied disciplines.
    2007;48(3–4):224–44. doi:10.1111/j.1469-7610.2007.01730.x.

    79. McGowan PO, Suderman M, Sasaki A, Huang TC, Hallett M, Meaney MJ,
    et al. Broad epigenetic signature of maternal care in the brain of adult rats.
    PLoS One. 2011;6(2):e14739. doi:10.1371/journal.pone.0014739.

    80. Kammerer M, Marks MN, Pinard C, Taylor A, von Castelberg B, Kunzli H, et al.
    Symptoms associated with the DSM IV diagnosis of depression in
    pregnancy and post partum. Archives of women’s mental health. 2009;12(3):
    135–41. doi:10.1007/s00737-009-0062-9.

    81. Plant DT, Pariante CM, Sharp D, Pawlby S. Maternal depression during
    pregnancy and offspring depression in adulthood: role of child
    maltreatment. The British journal of psychiatry : the journal of mental
    science. 2015;207(3):213–20. doi:10.1192/bjp.bp.114.156620.

    82. McGowan PO, Sasaki A, D’Alessio AC, Dymov S, Labonte B, Szyf M, et al.
    Epigenetic regulation of the glucocorticoid receptor in human brain
    associates with childhood abuse. Nat Neurosci. 2009;12(3):342–8. doi:10.
    1038/nn.2270.

    83. Oberlander TF, Weinberg J, Papsdorf M, Grunau R, Misri S, Devlin AM.
    Prenatal exposure to maternal depression, neonatal methylation of human
    glucocorticoid receptor gene (NR3C1) and infant cortisol stress responses.
    Epigenetics. 2008;3(2):97–106.

    84. Perroud N, Paoloni-Giacobino A, Prada P, Olie E, Salzmann A, Nicastro R,
    et al. Increased methylation of glucocorticoid receptor gene (NR3C1) in
    adults with a history of childhood maltreatment: a link with the severity and
    type of trauma. Transl Psychiatry. 2011;1:e59. doi:10.1038/tp.2011.60.

    85. Suderman M, Borghol N, Pappas JJ, Pinto Pereira SM, Pembrey M, Hertzman
    C, et al. Childhood abuse is associated with methylation of multiple loci in
    adult DNA. BMC Med Genet. 2014;7:13. doi:10.1186/1755-8794-7-13.

    86. Klengel T, Mehta D, Anacker C, Rex-Haffner M, Pruessner JC, Pariante CM,
    et al. Allele-specific FKBP5 DNA demethylation mediates gene-childhood
    trauma interactions. Nat Neurosci. 2013;16(1):33–41. doi:10.1038/nn.3275.

    87. Labonte B, Suderman M, Maussion G, Navaro L, Yerko V, Mahar I, et al.
    Genome-wide epigenetic regulation by early-life trauma. Arch Gen
    Psychiatry. 2012;69(7):722–31. doi:10.1001/archgenpsychiatry.2011.2287.

    88. Martin-Blanco A, Ferrer M, Soler J, Salazar J, Vega D, Andion O, et al.
    Association between methylation of the glucocorticoid receptor gene,
    childhood maltreatment, and clinical severity in borderline personality
    disorder. J Psychiatr Res. 2014;57:34–40. doi:10.1016/j.jpsychires.2014.06.011.

    89. Dammann G, Teschler S, Haag T, Altmuller F, Tuczek F, Dammann RH.
    Increased DNA methylation of neuropsychiatric genes occurs in borderline
    personality disorder. Epigenetics. 2011;6(12):1454–62. doi:10.4161/epi.6.12.
    18363.

    90. Perroud N, Salzmann A, Prada P, Nicastro R, Hoeppli ME, Furrer S, et al.
    Response to psychotherapy in borderline personality disorder and
    methylation status of the BDNF gene. Transl Psychiatry. 2013;3:e207. doi:10.
    1038/tp.2012.140.

    91. Perroud N, Zewdie S, Stenz L, Adouan W, Bavamian S, Prada P, et al.
    Methylation of serotonin receptor 3a in Adhd, borderline personality, and
    bipolar disorders: link with severity of the disorders and childhood
    maltreatment. Depression and anxiety. 2016;33(1):45–55. doi:10.1002/da.
    22406.

    92. Thaler L, Gauvin L, Joober R, Groleau P, de Guzman R, Ambalavanan A, et al.
    Methylation of BDNF in women with bulimic eating syndromes:
    associations with childhood abuse and borderline personality disorder. Prog

    Neuro-Psychopharmacol Biol Psychiatry. 2014;54:43–9. doi:10.1016/j.pnpbp.
    2014.04.010.

    93. Teschler S, Bartkuhn M, Kunzel N, Schmidt C, Kiehl S, Dammann G, et al. Aberrant
    methylation of gene associated CpG sites occurs in borderline personality
    disorder. PLoS One. 2013;8(12):e84180. doi:10.1371/journal.pone.0084180.

    94. Prados J, Stenz L, Courtet P, Prada P, Nicastro R, Adouan W, et al. Borderline
    personality disorder and childhood maltreatment: a genome-wide methylation
    analysis. Genes Brain Behav. 2015;14(2):177–88. doi:10.1111/gbb.12197.

    95. Teschler S, Gotthardt J, Dammann G, Dammann RH. Aberrant DNA Methylation
    of rDNA and PRIMA1 in Borderline Personality Disorder. International journal of
    molecular sciences. 2016;17(1). doi:10.3390/ijms17010067.

    96. Cattaneo A, Macchi F, Plazzotta G, Veronica B, Bocchio-Chiavetto L, Riva MA,
    et al. Inflammation and neuronal plasticity: a link between childhood
    trauma and depression pathogenesis. Front Cell Neurosci. 2015;9:40. doi:10.
    3389/fncel.2015.00040.

    97. Briggs JA, Wolvetang EJ, Mattick JS, Rinn JL, Barry G. Mechanisms of long
    non-coding RNAs in mammalian nervous system development, plasticity,
    disease, and evolution. Neuron. 2015;88(5):861–77. doi:10.1016/j.neuron.
    2015.09.045.

    98. McIntyre CK, McGaugh JL, Williams CL. Interacting brain systems modulate
    memory consolidation. Neurosci Biobehav Rev. 2012;36(7):1750–62. doi:10.
    1016/j.neubiorev.2011.11.001.

    99. Dhabhar FS. Enhancing versus suppressive effects of stress on immune
    function: implications for immunoprotection and immunopathology.
    Neuroimmunomodulation. 2009;16(5):300–17. doi:10.1159/000216188.

    100. McEwen BS. Physiology and neurobiology of stress and adaptation: central role
    of the brain. Physiol Rev. 2007;87(3):873–904. doi:10.1152/physrev.00041.2006.

    101. McEwen BS. Understanding the potency of stressful early life experiences
    on brain and body function. Metab Clin Exp. 2008;57(Suppl 2):S11–5. doi:10.
    1016/j.metabol.2008.07.006.

    102. McEwen BS. Protection and damage from acute and chronic stress: allostasis
    and allostatic overload and relevance to the pathophysiology of psychiatric
    disorders. Ann N Y Acad Sci. 2004;1032:1–7. doi:10.1196/annals.1314.001.

    103. Herbert J, Goodyer IM, Grossman AB, Hastings MH, de Kloet ER, Lightman
    SL, et al. Do corticosteroids damage the brain? J Neuroendocrinol. 2006;
    18(6):393–411. doi:10.1111/j.1365-2826.2006.01429.x.

    104. Duman RS, Monteggia LM. A neurotrophic model for stress-related mood
    disorders. Biol Psychiatry. 2006;59(12):1116–27. doi:10.1016/j.biopsych.2006.02.013.

    105. Kapczinski F, Frey BN, Andreazza AC, Kauer-Sant’Anna M, Cunha AB, Post
    RM. Increased oxidative stress as a mechanism for decreased BDNF levels in
    acute manic episodes. Rev Bras Psiquiatr. 2008;30(3):243–5.

    106. Waterhouse EG, Xu B. New insights into the role of brain-derived
    neurotrophic factor in synaptic plasticity. Mol Cell Neurosci. 2009;42(2):81–9.
    doi:10.1016/j.mcn.2009.06.009.

    107. Calabrese F, Molteni R, Gabriel C, Mocaer E, Racagni G, Riva MA. Modulation
    of neuroplastic molecules in selected brain regions after chronic
    administration of the novel antidepressant agomelatine.
    Psychopharmacology. 2011;215(2):267–75. doi:10.1007/s00213-010-2129-8.

    108. Ansorge MS, Hen R, Gingrich JA. Neurodevelopmental origins of depressive
    disorders. Curr Opin Pharmacol. 2007;7(1):8–17. doi:10.1016/j.coph.2006.11.
    006.

    109. Koenigsberg HW, Yuan P, Diaz GA, Guerreri S, Dorantes C, Mayson S, et al.
    Platelet protein kinase C and brain-derived neurotrophic factor levels in
    borderline personality disorder patients. Psychiatry Res. 2012;199(2):92–7.
    doi:10.1016/j.psychres.2012.04.026.

    110. Polyakova M, Stuke K, Schuemberg K, Mueller K, Schoenknecht P, Schroeter
    ML. BDNF as a biomarker for successful treatment of mood disorders: a
    systematic & quantitative meta-analysis. J Affect Disord. 2015;174:432–40.
    doi:10.1016/j.jad.2014.11.044.

    111. Cattaneo A, Bocchio-Chiavetto L, Zanardini R, Milanesi E, Placentino A,
    Gennarelli M. Reduced peripheral brain-derived neurotrophic factor mRNA
    levels are normalized by antidepressant treatment. The international journal
    of neuropsychopharmacology / official scientific journal of the Collegium
    Internationale Neuropsychopharmacologicum. 2010;13(1):103–8. doi:10.
    1017/S1461145709990812.

    112. Cattaneo A, Gennarelli M, Uher R, Breen G, Farmer A, Aitchison KJ, et al.
    Candidate genes expression profile associated with antidepressants
    response in the GENDEP study: differentiating between baseline ‘predictors’
    and longitudinal ‘targets’. Neuropsychopharmacology : official publication of
    the American College of Neuropsychopharmacology. 2013;38(3):377–85. doi:
    10.1038/npp.2012.191.

    Cattane et al. BMC Psychiatry (2017) 17:221 Page 14 of 14

    http://dx.doi.org/10.1503/jpn.120039

    http://dx.doi.org/10.1016/j.neuron.2015.05.036

    http://dx.doi.org/10.1016/j.neuron.2015.05.036

    http://dx.doi.org/10.1534/genetics.109.102798

    http://dx.doi.org/10.1016/j.nbd.2011.09.005

    http://dx.doi.org/10.1038/nrn3879

    http://dx.doi.org/10.1111/j.1469-7610.2007.01730.x

    http://dx.doi.org/10.1371/journal.pone.0014739

    http://dx.doi.org/10.1007/s00737-009-0062-9

    http://dx.doi.org/10.1192/bjp.bp.114.156620

    http://dx.doi.org/10.1038/nn.2270

    http://dx.doi.org/10.1038/nn.2270

    http://dx.doi.org/10.1038/tp.2011.60

    http://dx.doi.org/10.1186/1755-8794-7-13

    http://dx.doi.org/10.1038/nn.3275

    http://dx.doi.org/10.1001/archgenpsychiatry.2011.2287

    http://dx.doi.org/10.1016/j.jpsychires.2014.06.011

    http://dx.doi.org/10.4161/epi.6.12.18363

    http://dx.doi.org/10.4161/epi.6.12.18363

    http://dx.doi.org/10.1038/tp.2012.140

    http://dx.doi.org/10.1038/tp.2012.140

    http://dx.doi.org/10.1002/da.22406

    http://dx.doi.org/10.1002/da.22406

    http://dx.doi.org/10.1016/j.pnpbp.2014.04.010

    http://dx.doi.org/10.1016/j.pnpbp.2014.04.010

    http://dx.doi.org/10.1371/journal.pone.0084180

    http://dx.doi.org/10.1111/gbb.12197

    http://dx.doi.org/10.3390/ijms17010067

    http://dx.doi.org/10.3389/fncel.2015.00040

    http://dx.doi.org/10.3389/fncel.2015.00040

    http://dx.doi.org/10.1016/j.neuron.2015.09.045

    http://dx.doi.org/10.1016/j.neuron.2015.09.045

    http://dx.doi.org/10.1016/j.neubiorev.2011.11.001

    http://dx.doi.org/10.1016/j.neubiorev.2011.11.001

    http://dx.doi.org/10.1159/000216188

    http://dx.doi.org/10.1152/physrev.00041.2006

    http://dx.doi.org/10.1016/j.metabol.2008.07.006

    http://dx.doi.org/10.1016/j.metabol.2008.07.006

    http://dx.doi.org/10.1196/annals.1314.001

    http://dx.doi.org/10.1111/j.1365-2826.2006.01429.x

    http://dx.doi.org/10.1016/j.biopsych.2006.02.013

    http://dx.doi.org/10.1016/j.mcn.2009.06.009

    http://dx.doi.org/10.1007/s00213-010-2129-8

    http://dx.doi.org/10.1016/j.coph.2006.11.006

    http://dx.doi.org/10.1016/j.coph.2006.11.006

    http://dx.doi.org/10.1016/j.psychres.2012.04.026

    http://dx.doi.org/10.1016/j.jad.2014.11.044

    http://dx.doi.org/10.1017/S1461145709990812

    http://dx.doi.org/10.1017/S1461145709990812

    http://dx.doi.org/10.1038/npp.2012.191

    Reproduced with permission of copyright owner.
    Further reproduction prohibited without permission.

      Abstract

      Background

      Discussion

      Summary

      Background

      Discussion

      Neurobiological mechanisms involved in BPD

      BPD and the hypothalamic-pituitary-adrenal axis

      BPD and neurotransmission

      BPD and the endogenous opioid system

      BPD and neuroimaging studies

      Volumetric alterations in brain areas involved in stress response

      Endogenous opiod system alterations in brain regions involved in stress response

      BPD and epigenetic mechanisms

      BPD and neuroplasticity (the role of BDNF)

      Conclusions

      Abbreviations

      Acknowledgements

      Funding

      Availability of data and materials

      Authors’ contributions

      Competing interests

      Consent for publication

      Ethics approval and consent to participate

      Author details

      References

    Full Terms & Conditions of access and use can be found at
    https://www.tandfonline.com/action/journalInformation?journalCode=zept20

    European Journal of Psychotraumatology

    ISSN: 2000-8198 (Print) 2000-8066 (Online) Journal homepage: https://www.tandfonline.com/loi/zept20

    Distinguishing PTSD, Complex PTSD, and
    Borderline Personality Disorder: A latent class
    analysis

    Marylène Cloitre, Donn W. Garvert, Brandon Weiss, Eve B. Carlson & Richard
    A. Bryant

    To cite this article: Marylène Cloitre, Donn W. Garvert, Brandon Weiss, Eve B. Carlson & Richard
    A. Bryant (2014) Distinguishing PTSD, Complex PTSD, and Borderline Personality Disorder:
    A latent class analysis, European Journal of Psychotraumatology, 5:1, 25097, DOI: 10.3402/
    ejpt.v5.25097

    To link to this article: https://doi.org/10.3402/ejpt.v5.25097

    © 2014 Marylène Cloitre et al. View supplementary material

    Published online: 15 Sep 2014. Submit your article to this journal

    Article views: 35822 View related articles

    View Crossmark data Citing articles: 94 View citing articles

    https://www.tandfonline.com/action/journalInformation?journalCode=zept20

    https://www.tandfonline.com/loi/zept20

    https://www.tandfonline.com/action/showCitFormats?doi=10.3402/ejpt.v5.25097

    https://www.tandfonline.com/action/showCitFormats?doi=10.3402/ejpt.v5.25097

    https://doi.org/10.3402/ejpt.v5.25097

    https://www.tandfonline.com/doi/suppl/10.3402/ejpt.v5.25097

    https://www.tandfonline.com/doi/suppl/10.3402/ejpt.v5.25097

    https://www.tandfonline.com/action/authorSubmission?journalCode=zept20&show=instructions

    https://www.tandfonline.com/action/authorSubmission?journalCode=zept20&show=instructions

    https://www.tandfonline.com/doi/mlt/10.3402/ejpt.v5.25097

    https://www.tandfonline.com/doi/mlt/10.3402/ejpt.v5.25097

    http://crossmark.crossref.org/dialog/?doi=10.3402/ejpt.v5.25097&domain=pdf&date_stamp=2014-09-15

    http://crossmark.crossref.org/dialog/?doi=10.3402/ejpt.v5.25097&domain=pdf&date_stamp=2014-09-15

    https://www.tandfonline.com/doi/citedby/10.3402/ejpt.v5.25097#tabModule

    https://www.tandfonline.com/doi/citedby/10.3402/ejpt.v5.25097#tabModule

    CLINICAL RESEARCH ARTICLE

    Distinguishing PTSD, Complex PTSD, and Borderline
    Personality Disorder: A latent class analysis

    Marylène Cloitre1,2*, Donn W. Garvert1, Brandon Weiss1,3, Eve B. Carlson

    1

    and Richard A. Bryant4

    1National Center for PTSD, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA;
    2Department of Psychiatry and Child & Adolescent Psychiatry, New York University Medical Center,
    New York, USA; 3Department of Psychiatry and Behavioral Sciences, Stanford University School
    of Medicine, Palo Alto, CA, USA; 4School of Psychology, University of New South Wales, Sydney,
    NSW, Australia

    Background: There has been debate regarding whether Complex Posttraumatic Stress Disorder (Complex

    PTSD) is distinct from Borderline Personality Disorder (BPD) when the latter is comorbid with PTSD.

    Objective: To determine whether the patterns of symptoms endorsed by women seeking treatment for child-

    hood abuse form classes that are consistent with

    diagnostic criteria for PTSD, Complex PTSD, and BPD.

    Method: A latent class analysis (LCA) was conducted on an archival dataset of 280 women with histories of

    childhood abuse assessed for enrollment in a clinical trial for PTSD.

    Results: The LCA revealed four distinct classes of individuals: a Low Symptom class characterized by low

    endorsements on all symptoms; a PTSD class characterized by elevated symptoms of PTSD but low endorse-

    ment of symptoms that define the Complex PTSD and BPD diagnoses; a Complex PTSD class characterized by

    elevated symptoms of PTSD and self-organization symptoms that defined the Complex PTSD diagnosis but

    low on the symptoms of BPD; and a BPD class characterized by symptoms of BPD. Four BPD symptoms were

    found to greatly increase the odds of being in the BPD compared to the Complex PTSD class: frantic efforts to

    avoid abandonment, unstable sense of self, unstable and intense interpersonal relationships, and impulsiveness.

    Conclusions: Findings supported the construct validity of Complex PTSD as distinguishable from BPD. Key

    symptoms that distinguished between the disorders were identified, which may aid in differential diagnosis

    and treatment planning.

    Keywords: Complex PTSD; posttraumatic stress disorder; Borderline Personality Disorder; WHO; ICD-11

    *Correspondence to: Marylène Cloitre, National Center for PTSD Dissemination and Training Division,

    VAPAHCS, 795 Willow Road, Menlo Park, CA 94025, USA, Email: Marylene.cloitre@nyumc.org

    For the abstract or full text in other languages, please see Supplementary files under Article Tools online

    Received: 3 June 2014; Revised: 22 July 2014; Accepted: 18 August 2014; Published: 15 September 2014

    T
    here has long been debate about whether Complex

    Posttraumatic Stress Disorder (Complex PTSD)

    is distinct from Borderline Personality Disorder

    (BPD) comorbid with PTSD. Part of the difficulty in this

    evaluation has been the lack of clear and consistent

    characterization of Complex PTSD. The World Health

    Organization (WHO) Working Group on the Classification

    of Stress-Related Disorders has proposed the inclusion

    of Complex PTSD as a new diagnosis related to but sepa-

    rate from PTSD (Maercker et al., 2013). Both of these

    disorders are viewed as distinct and separate from BPD.

    An emerging and accumulating empirical literature is

    demonstrating consistent and clear differences between

    ICD-11 PTSD and Complex PTSD. In addition, it is

    important to determine the construct validity of Complex

    PTSD as empirically distinct from BPD particularly

    among those with a trauma history. This investigation

    evaluated whether ICD-11 Complex PTSD could be dis-

    tinguished from DSM-IV BPD in a treatment-seeking

    population of women with childhood abuse.

    The WHO proposed that the development of ICD-11

    be guided by the principle of clinical utility. Characteris-

    tics of clinical utility include the organization of disorders

    that are consistent with clinicians’ mental health taxo-

    nomies, that contain a limited number of symptoms so

    that they can be easily recalled and used in the field, and

    that are based on distinctions important for manage-

    ment and treatment (Reed, 2010). The distinction between

    PSYCHOTRAUMATOLOGY
    EUROPEAN JOURNAL OF

    European Journal of Psychotraumatology 2014. # 2014 Marylène Cloitre et al. This is an Open Access article distributed under the terms of the Creative Commons
    Attribution 4.0 Unported (CC-BY 4.0) License (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or
    format, and to remix, transform, and build upon the material, for any purpose, even commercially, under the condition that appropriate credit is given, that a link to the license
    is provided, and that you indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

    1

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    (page number not for citation purpose)

    http://www.eurojnlofpsychotraumatol.net/index.php/ejpt/rt/suppFiles/25097/0

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    ICD-11 PTSD and Complex PTSD are consistent with

    these guidelines (see Cloitre, Garvert, Brewin, Bryant, &

    Maercker, 2013; Maercker et al., 2013). For example,

    ICD-11 PTSD is construed as a fear-based disorder and

    symptoms are limited to and consistent with fear reactions

    and consequent avoidance and hypervigilence. In contrast,

    Complex PTSD has been described as typically associated

    with chronic and repeated traumas and includes not only

    the symptoms of PTSD but also disturbances in self-

    organization reflected in emotion regulation, self-concept

    and relational difficulties (see Cloitre et al., 2013) a symp-

    tom profile that has been demonstrated as associated

    with prolonged trauma (Briere & Rickards, 2007).

    Three studies have found evidence supporting the vali-

    dity of the ICD-11 PTSD versus Complex PTSD distinction

    (see Table 1 for description of the diagnoses). Recently, in

    order to evaluate whether PTSD and Complex PTSD

    could be empirically distinguished from each other, Cloitre

    and colleagues (2013) performed a latent profile analysis

    (LPA) on assessment data from 302 treatment-seeking

    individuals with diverse trauma histories, ranging from

    single events (e.g., 9/11 attacks) to sustained exposures

    (e.g., childhood or adult physical and/or sexual abuse).

    The results were consistent with the ICD-11 formulation

    for Complex PTSD, with the best fitting LPA model

    delineating three classes of individuals: (1) a Complex

    PTSD class, with high levels of both PTSD symptoms as

    well as disturbances in self-organization related to affect

    regulation problems, negative self-concept, and relational

    difficulties; (2) a PTSD class, with high levels of PTSD

    symptoms but relatively low on the disturbances in self-

    organization that define Complex PTSD; and (3) a class

    Table 1. Symptom profile for each diagnosis and items used in the LCA analyses

    Symptoms for each diagnoses

    ICD-11 PTSD ICD-11 CPTSD DSM-IV BPD Items

    Re-experiencing

    Re-experiencing

    Flashbacks Flashbacks CAPS 1: Unwanted memories of the event

    Nightmares Nightmares CAPS 2: Recurrent distressing dreams of the event

    Avoidance

    Avoidance

    Thoughts Thoughts CAPS 6: Avoid thoughts, feelings or conversations

    People, places,

    activities

    People, places, activities CAPS 7: Avoid activities, places, or people

    Sense of threat

    Sense of threat

    Hypervigilance Hypervigilance CAPS 16: Being especially alert constantly on guard

    Startle Startle CAPS 17: Strong startle reactions

    Emotion regulation

    Anger BSI 13: Temper outburst that you could not control

    Hurt Feelings BSI 20: Your feelings being easily hurt

    Negative self-concept

    Worthless BSI 50: Feeling of worthlessness

    Guilty BSI 52: Feelings of guilt

    Interpersonal problems

    Not close BSI 44: Never feeling close to another person

    Feel disconnected CAPS 10: Feeling distant or cut off from other people

    Frantic SCID-II 90: Frantic efforts to avoid abandonment

    Unstable relationships SCID-II 91: Unstable and intense relationships with alternating

    extremes of idealization and devaluation

    Unstable sense of self SCID-II 92: Markedly and persistently unstable sense of self

    Impulsiveness SCID-II 96: Impulsiveness that is potentially self-damaging

    Self-harm SCID-II 97: Recurrent suicidal behavior, gestures, or threats,

    or self-mutilating behavior

    Mood changes SCID-II 99: Affective instability due to reactivity to mood

    Empty SCID-II 100: Chronic feelings of emptiness

    Temper SCID-II 101: Frequent displays of anger, constant anger,

    recurrent physical fights

    Paranoid/dissociation SCID-II 104: Transient, stress-related paranoid ideation or

    severe dissociative symptoms

    Marylène Cloitre et al.

    2
    (page number not for citation purpose)

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    relatively low on symptoms of both PTSD and Complex

    PTSD. Notably, these identified classes were identical

    when including an additional 86 participants with BPD,

    providing further support for the stability of the identified

    classes. Cloitre et al. (2013) also found that chronic trauma

    was more predictive of Complex PTSD than PTSD and

    that Complex PTSD resulted in significantly greater func-

    tional impairment than PTSD.

    Elklit, Hyland, and Shevlin (2014) replicated the find-

    ings of Cloitre and colleagues (2013), performing a latent

    class analysis (LCA) on three separate samples of trauma-

    exposed Danish individuals who experienced primary

    traumas of bereavement, sexual assault, and physical

    assault. The investigators found that the LCA model with

    the best fit for each sample consisted of three classes of

    individuals identical to those identified by Cloitre et al.

    (2013). Lastly, Knefel and Lueger-Schuster (2013) per-

    formed confirmatory factor analysis (CFA) on data from

    226 Austrian adults who had experienced institutional

    abuse, defined as physical, sexual or emotional abuse

    by individuals representing institutions responsible for

    the care of children (i.e., Catholic Church, foster care).

    Results indicated that individuals diagnosed with Com-

    plex PTSD had experienced significantly longer exposure

    to traumatic situations and that the theoretically derived

    CFA model demonstrated good model fit. Overall, these

    studies provide substantial support for the construct vali-

    dity of Complex PTSD across international samples of

    individuals exposed to diverse traumatic events, demon-

    strating that it is a diagnostic entity distinct from PTSD

    and supporting the recommendations of the ICD-11

    proposal.

    The argument that Complex PTSD is an amalgam of

    PTSD and BPD has been built on reports of the relatively

    high comorbidity between PTSD and BPD. For example,

    using data from the National Epidemiologic Survey on

    Alcohol and Related Conditions (NESARC), a nationally

    representative sample of United States population, Pagura

    and colleagues (2010) found that 24% of individuals with

    lifetime PTSD also met criteria for BPD, 30% of indi-

    viduals with BPD also met criteria for lifetime PTSD,

    and 2% had comorbid PTSD and BPD. Also utilizing

    NESARC data, Grant and colleagues (2008) found that

    29% of individuals who currently met criteria for PTSD

    in the past 12 months also met criteria for lifetime BPD,

    and 32% of individuals with lifetime BPD met criteria for

    12-month PTSD. In clinical samples, the rates of comor-

    bidity are higher. PTSD patients are reported to have

    BPD comorbidity ranging from 37 to 68% (Heffernan &

    Cloitre, 2000; Zlotnick, Franklin, & Zimmerman, 2002)

    and conversely, among BPD patients 25�58% are diag-

    nosed with comorbid PTSD (Golier et al., 2003; Harned,

    Rizvi, & Linehan, 2010; Zanarini et al., 1998).

    Despite these high rates of comorbidity, the key clinical

    features of Complex PTSD and BPD differ and lead to

    different treatment implications, a consequence of signifi-

    cance when considering the clinical utility of diagnostic

    formulation. Complex PTSD includes PTSD symptoms

    and, accordingly, treatment highlights the amelioration

    of the trauma memory as a key goal (Cloitre et al., 2011).

    In contrast, the key impairing features of BPD are self-

    injurious and suicidal behaviors, and treatment activities

    focus on the resolution of these behaviors (e.g., Linehan,

    1993). There are several other ways in which the profile of

    Complex PTSD differs from that of BPD. First, it should

    be noted that BPD does not require a traumatic stressor

    for diagnosis and PTSD symptoms may or may not be

    present. Rather, BPD is characterized by fear of abandon-

    ment, shifting self-image or self-concept, shifting ideali-

    zation and devaluation in relationships, and frequent

    impulsive and suicidal behaviors (see Table 1). In Complex

    PTSD, as proposed in ICD-11, the fear of abandonment

    is not a requirement of the disorder, self-identify is

    consistently negative rather than shifting and relational

    disturbances highlight chronic avoidance of relationships

    rather than sustained chaotic engagement. While emotion

    regulation difficulties are central to both Complex PTSD

    and BPD, their expression is quite different. In Complex

    PTSD they predominantly include emotional sensitivity,

    reactive anger and poor coping responses (e.g., use of

    alcohol and substances). In BPD, some of the preceding

    may be observed, but the criteria, perhaps the defining

    characteristics of the disorder, include suicide attempts

    and gestures as well as self-injurious behaviors, events

    which occur much less frequently in complex forms of

    PTSD than in BPD samples (e.g., Zlotnick et al., 2002).

    Given these identified differences in diagnostic formula-

    tion and their treatment implications, empirical evaluation

    of Complex PTSD and BPD in a symptom-by-symptom

    manner is important.

    The purpose of the current study was to determine

    whether the symptoms endorsed by women seeking treat-

    ment for childhood abuse form classes that are consistent

    with diagnostic criteria for PTSD, Complex PTSD, and

    BPD (see Table 1). We hypothesized that analyses would

    reveal at least three distinct classes of individuals with the

    following symptom profiles: (1) high levels of ICD-11

    PTSD symptoms but not symptoms of Complex PTSD or

    BPD, (2) high levels of Complex PTSD symptoms (PTSD

    plus emotion regulation, negative self-concept and inter-

    personal problems) but not BPD symptoms; (3) high

    levels of BPD symptoms with an admixture of trauma-

    related symptoms (e.g., PTSD, CPTSD symptoms).

    Methods

    Participants and procedures
    The data for these analyses were obtained from an archi-

    val set of measures administered as part of an assessment

    procedure for a randomized controlled trial for PTSD

    PTSD, Complex PTSD, and BPD

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097 3
    (page number not for citation purpose)

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    related to childhood abuse (n�310) (see Cloitre et al.,

    2010). The data are a subset of individuals for whom com-

    plete measures of PTSD, BPD, general psychopathology,

    and functional impairment were available (n�280).

    Participants had a mean age of 37.13 (SD �10.86)

    years. The entire sample was female and 40% identified

    as Caucasian (40.4%, n�113), followed by African-

    American (26.4%, n�74), Hispanic (18.6%, n�52), Asian

    (3.9%, n�11), other (8.6%, n�24), and unreported

    (2.1%, n�6). Marital status of the sample was as follows:

    54.3% (n�152) reported being single, married (11.1%,

    n�31), divorced or separated (16.1%, n�45), living with

    a significant other (15.7%, n�44), widowed (0.7%, n�2),

    and unreported (2.1%, n�6). College graduation or com-

    pletion of some college was reported by 64.3% (180),

    postgraduate education was reported by 24.3% (68), high

    school graduation or some high school was reported by

    9.3% (26), and education level was unavailable for 2.1%

    (6). The majority of participants reported some employ-

    ment with 41.4% (116) employed full-time (35� hours

    per week) and 23.9% (n�67) employed part-time (B35

    hours per week).

    Frequency of traumas were as follows: childhood sexual

    abuse (CSA) (65.1%), childhood physical abuse (CPA)

    (80.8%), neglect (46.4%), emotional abuse (80.4%), 35.9%

    were not living with their mother before the age of 18,

    adulthood sexual assault (ASA) (49.6%), and adult-

    hood physical assault (APA) (26.0%). All individuals

    had experienced either CPA or CSA.

    Measures

    Clinician Administered PTSD Scale

    The Clinician Administered PTSD Scale (CAPS) for

    DSM-IV PTSD is a well-validated clinician administered

    interview (see Weathers, Keane, & Davidson, 2001) that

    evaluates the presence and severity of the 17 DSM-IV

    PTSD symptoms over the past month with separate five-

    point scales for frequency (ranging from 0 � ‘‘never’’ to

    4 � ‘‘almost daily’’) and intensity (ranging from 0 �
    ‘‘none’’ to 4 � ‘‘extreme’’). The CAPS items used for

    current analyses are listed on Table 1. An item with a

    frequency score of 1 (‘‘once or twice in the past month’’)

    or higher and an intensity score of 2 (‘‘moderate’’) or

    higher was considered positive for that symptom follow-

    ing guidelines suggested by Weathers et al. (2001).

    Brief Symptom Inventory

    The Brief Symptom Inventory (BSI) is a 53-item self-

    report psychological symptom inventory with nine pri-

    mary symptom dimensions. The measure assesses how

    much a problem bothered or distressed a person using a

    5-point Likert scale ranging from 0 � ‘‘not at all’’ and

    4 � ‘‘extremely’’. The BSI has shown high convergent

    and construct validity (Derogatis & Melisaratos, 1983).

    The BSI items used for the current analyses are listed on

    Table 1. An item score of 2 (‘‘moderately’’) or higher was

    considered positive for

    that symptom.

    Structured Clinical Interview-II Borderline Personality
    Disorder module

    The Structured Clinical Interview for Axis II Disorders

    (SCID-II) DSM�IV BPD Module has nine items where

    a score of 1�absent or false, 2�subthreshold and

    3�threshold or true (First, Spitzer, Gibbon, & Williams,

    1994). The items used for the current analyses are listed

    on Table 1. An item score of 3 was considered positive for

    that symptom.

    Social Adjustment Scale-Self Report

    The Social Adjustment Scale-Self Report (SAS-SR;

    Weissman & Bothwell, 1976) was utilized to measure func-

    tional impairment. The SAS-SR consists of 42 Likert-

    type items, which assess the level of functioning over the

    past 2 weeks for six domains: work, social and leisure

    activities, relationships with extended family, role as a

    marital partner, parental role, and role within the family

    unit. A mean score can be calculated for each of the six

    domains, as well as one overall mean score, based on the

    total number of items relevant to the responder. Higher

    scores indicate greater impairment. The SAS-SR has

    demonstrated strong psychometric properties among com-

    munity and clinical samples (e.g., Weissman & Bothell,

    1976).

    Statistical analyses

    Latent class analysis

    The model fit for the optimal number of classes that were

    examined were the Lo-Mendell-Rubin adjusted likeli-

    hood ratio test (LMR-A), the bootstrap likelihood ratio

    test (BLRT), the Bayesian Information Criterion (BIC),

    the Sample-Size Adjusted BIC (SSA-BIC), and the Akaike

    Information Criterion (AIC). In a simulation study, the

    BLRT was shown to outperform the LMR-A and the

    BIC (among other measures of model fit) in selecting

    the number of classes (Nylund, Asparouhov, & Muthen,

    2007). Since there is not a clear-cut decision rule on how

    to select the best fitting model, we ranked ordered the

    importance of fit indices as follows: BLRT, BIC, SSA-

    BIC, AIC, and then the LMR-A. The general practice of

    LCA is to test the fit of a two-class model and system-

    atically increase the number of classes until adding more

    classes is no longer warranted. The LMR-A and the

    BLRT compare the fit of the specified class solution to

    models with one less class. A pB0.05 suggests that the

    specified model provides a better fit to the data relative to

    the model with one less class. A total of 21 symptoms

    (coded dichotomously as present or not) were used in

    the LCA, 6 representing the ICD-11 PTSD symptoms,

    6 representing the self-organization symptoms unique

    to Complex PTSD), and 9 representing the SCID-II1

    Marylène Cloitre et al.

    4
    (page number not for citation purpose)

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    symptoms of BPD (see Table 1). The LCA models were

    estimated using robust maximum likelihood method with

    400 initial stage random starts and 80 final stage opti-

    mizations to determine if the best log-likelihood value

    was obtained and replicated. Finally, 50 bootstrap draws

    were used in the BLRT.

    Descriptive statistics

    Chi-square tests and ANOVAs were performed to assess

    differences in sociodemographic characteristics, trauma

    history, and symptom severity across the classes identi-

    fied in the LCA. Descriptive statistics were computed

    based on valid (non-missing) data.

    Results

    Latent class analysis

    The fit indices of the different class models examined are

    shown in Table 2. The two-class model yielded a signi-

    ficant LMR-A and BLRT result at pB0.05. The three-

    and four-class models both yielded a significant BLRT

    result at pB0.05, but not a significant LMR-A result.

    A five-class model was examined, but the best log-

    likelihood value was not replicated, and it was not con-

    sidered for the final model, as the p-value may not be

    trustworthy due to local maxima. The four-class model

    did not have the lowest BIC value, but it was selected over

    the two- and three-class models because it was the model

    with the largest number of classes that had a significant

    and trustworthy BLRT result, had the lowest SSA-BIC

    value, and had the lowest AIC value of the models consi-

    dered. The three- and four-class models were examined

    closely, as they both could have legitimate arguments for

    being selected. However, based on all of the fit indices

    examined and on the interpretability of the symptom

    profiles of the classes (consistent with study hypotheses),

    the four-class model was selected.

    The pattern of symptom endorsement for all four classes

    is presented in Fig. 1. The four classes were compared

    on the 21 symptoms that were used to determine class

    membership in order to provide descriptive labels for

    each class. The Low Symptom class had relatively low

    levels of all symptoms across all domains. The PTSD class

    had generally high levels of PTSD symptoms, but relati-

    vely low levels of self-organization and BPD symptoms.

    The CPTSD class had high levels of PTSD and self-

    organization symptoms, but relatively low levels of BPD

    symptoms. The BPD class had a high percentage of BPD

    symptoms as well as self-organization disturbances and

    PTSD symptoms.

    The average probability of latent class membership in

    the four-class model was acceptable at 0.91 for the Low

    Symptom class, 0.92 for the PTSD class, 0.87 for the

    CPTSD class, and 0.91 for the BPD class, which implies

    acceptable discrimination between the classes. An accep-

    table entropy value probability of 0.81 lends support to

    this result by suggesting adequate latent class separation.

    Overall, 20.4% (n�57) of participants were classified

    into the Low Symptom class, 25.7% (n�72) into the

    PTSD class, 27.5% (n�77) into the CPTSD class, and

    26.4% (n�74) into the BPD class.

    Sociodemographic and trauma history characteristics

    ANOVA and Chi-square analyses were performed to assess

    differences in sociodemographic characteristics, trauma

    history, and symptom severity across the classes identi-

    fied in the LCA. Results shown in Table 3 indicate that

    the four classes did not differ by age, ethnicity, or employ-

    ment status. The classes also did not differ in the rates of

    types of childhood or adulthood interpersonal traumas,

    with the exception that CSA was reported more fre-

    quently in the CPTSD class than in the Low Symptom

    and BPD classes. Total number of different types of

    traumatic experiences did not differ across classes.

    Symptom characteristics

    The rates of probable disorders (ICD-11 PTSD, ICD-11

    CPTSD and BPD) as well as the percent of endorsed

    symptom characteristics for all 21 symptoms across the

    four classes are presented in Table 4. Overall, 53.9%

    (n�151) had a PTSD diagnosis, 38.2% (n�107) had a

    CPTSD diagnosis, and 29.3% (n�82) had a BPD

    diagnosis. Of those with a BPD diagnosis, majority also

    had a PTSD diagnosis (54.9%, n�45) and 45.1% (n�37)

    had a CPTSD diagnosis. In the Low Symptom class, no

    one met criteria for either PTSD or CPTSD while 12%

    Table 2. Latent class models and fit indices

    Model Log-likelihood BIC SSA-BIC AIC Entropy LMR-A p-value BLRT p-value

    2 classes �3523.010 7288.315 7151.965 7132.020 0.781 0.029 B0.001

    3 classes �3433.024 7232.310 7026.199 6996.048 0.817 0.066 B0.001

    4 classes �3382.025 7254.278 6978.406 6938.051 0.808 0.401 B0.001

    5 classes �3338.211 7290.613 6944.981 6894.421 0.848 0.639 B0.001a

    Note. BIC, Bayesian Information Criterion; SSA-BIC, Sample-Size Adjusted BIC; AIC, Akaike Information Criterion; LMRA-A, Lo-Mendell-

    Rubin adjusted likelihood ratio test; BLRT, bootstrap likelihood ratio test.
    aThe best log-likelihood value was not replicated in 32 out of 50 bootstrap draws. The p-value may not be trustworthy due to local
    maxima.

    PTSD, Complex PTSD, and BPD

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097 5
    (page number not for citation purpose)

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    met criteria for BPD. The most common symptoms were

    unstable relationships, mood changes and feeling empty.

    Of individuals in the PTSD class, 68% met criteria for

    PTSD, but only 19.4% met criteria for CPTSD and 1.4%

    met criteria for BPD. Of individuals in the CPTSD class,

    77.9% met criteria for CPTSD but only 7.8% met criteria

    for BPD. Of those in the BPD class, 91.9% met DSM-IV

    BPD diagnosis. Overall, the DSM-IV BPD diagnosis fit

    very few of the individuals in the CPTSD (7.8%) class but

    the large majority of the BPD class (91.9%).

    A review of the individual items indicates that,

    consistent with the graphic depiction provided in Fig. 1,

    the BPD class had a lower rate of endorsement of the

    ICD-11 PTSD symptoms across all items as compared to

    the CPTSD class. The rates were significantly lower for

    nightmares and avoidance of trauma-related thoughts.

    Endorsement of the individual items reflecting disturban-

    ces in self-organization by the BPD class members was

    similar to that of the CPTSD class. However, only 44.6%

    of the BPD class met criteria for CPTSD suggesting that

    Fig. 1. Symptom endorsement of Complex PTSD and BPD items by class.

    Table 3. Demographic and trauma characteristics of the classes

    Characteristics

    Class 1 Low Symptom

    n�57

    Class 2 PTSD

    n�72

    Class 3 CPTSD

    n�77

    Class 4 BPD

    n�74

    Significance

    test

    Age 37.91 (10.17) 36.21 (10.64) 36.95 (10.42) 37.63 (12.10) NS

    Race (% white) 44.4% 42.9% 33.8% 45.2% NS

    Employed (FT or PT) 66.0% 71.0% 68.4% 64.4% NS

    CSA 54.5% 66.7% 80.5% 55.4% p�0.003

    3�1, 4

    CPA 80.0% 82.9% 81.8% 78.4% NS

    Neglect 34.5% 47.1% 54.5% 45.9% NS

    Emotional abuse 78.2% 75.7% 79.2% 87.8% NS

    Any childhood abuse 98.2% 98.6% 97.4% 95.9% NS

    ASA 38.9% 46.4% 57.1% 52.7% NS

    APA 29.6% 18.8% 32.5% 23.3% NS

    Any adult assaults 53.7% 55.9% 72.7% 65.8% NS

    Both child and adult events 51.9% 55.9% 71.4% 63.5% NS

    Note. All tests were Chi-square tests with 3 degrees of freedom.

    Marylène Cloitre et al.

    6
    (page number not for citation purpose)

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    individuals did not consistently endorse the CPTSD symp-

    toms across all three categories of disturbance (emotion

    dysregulation, negative self-concept, interpersonal pro-

    blems) sufficient to complete the CPTSD profile. Indeed,

    individuals in the BPD class were more likely to meet

    criteria for PTSD (54%) than CPTSD.

    The CPTSD class had significantly lower endorsement

    of all the BPD symptoms than the BPD with the excep-

    tion of feelings of emptiness. The CPTSD class was more

    similar to the PTSD class in regard to endorsement of

    BPD symptoms. The two classes did not differ from each

    other on the BPD symptoms in seven out of nine symp-

    toms, with the exception of unstable relationships and

    mood changes, which were both endorsed at higher rates

    in the CPTSD class than the PTSD class. Notably, almost

    half of the BPD class members endorsed self-harm/

    Table 4. Frequencies of endorsement for ICD-11 PTSD, CPTSD, and DSM-IV BPD items

    Symptoms

    Class 1 Low symptoms

    (n�57)

    Class 2 PTSD

    (n�72)

    Class 3 CPTSD

    (n�77)

    Class 4 BPD

    (n�74)

    Significant pairwise

    post-hoc comparisons

    ICD-11 PTSD diagnosis 0.0% 68.1% 80.5% 54.1% 2, 3, 4�1

    3�4

    Re-experiencing

    Flashbacks 15.8% 80.6% 80.5% 75.7% 2, 3, 4�1

    Nightmares 3.5% 45.8% 70.1% 41.9% 2, 3, 4�1

    3�2, 4

    Avoidance

    Thoughts 10.5% 90.3% 89.6% 58.1% 2, 3, 4�1

    2, 3�4

    People, places, or activities 10.5% 66.7% 67.5% 50.0% 2, 3, 4�1

    Sense of threat

    Hypervigilance 21.1% 70.8% 75.3% 63.5% 2, 3, 4�1

    Startle 26.3% 51.4% 70.1% 60.8% 2, 3, 4�1

    ICD-11 CPTSD diagnosis 0.0% 19.4% 77.9% 44.6% 3�1, 2,4

    4�1, 2

    Affect regulation problems

    Angry 28.1% 23.6% 54.6% 51.4% 3, 4�1, 2

    Hurt feelings 54.4% 51.4% 97.4% 87.8% 3, 4�1, 2

    Negative self-concept

    Worthless 40.4% 20.8% 93.5% 87.8% 3, 4�1, 2

    Guilty 54.4% 43.1% 92.2% 81.1% 3, 4�1, 2

    Interpersonal problems

    Not close 36.8% 29.2% 83.1% 70.3% 3, 4�1, 2

    Feel disconnected 59.7% 76.4% 98.7% 85.1% 3, 4�1

    3�2, 4

    DSM-IV BPD diagnosis 12.3% 1.4% 7.8% 91.9% 4�1, 2, 3

    Frantic 19.3% 15.3% 11.7% 63.5% 4�1, 2, 3

    Unstable relationships 43.9% 8.3% 33.8% 83.8% 4�1, 2, 3

    1, 3�2

    Unstable sense of self 21.1% 2.8% 14.3% 67.6% 4�1, 2, 3

    1�2

    Impulsiveness 26.3% 16.7% 22.1% 73.0% 4�1, 2, 3

    Self-harm 0.0% 16.7% 14.3% 48.7% 4�1, 2, 3

    2, 3�1

    Mood changes 40.4% 8.3% 41.6% 73.0% 4�1, 2, 3

    1, 3�2

    Empty 31.6% 22.2% 81.8% 78.4% 3, 4�1, 2

    Temper 22.8% 15.3% 15.6% 59.5% 4�1, 2, 3

    Paranoia/dissociation 21.1% 16.7% 32.5% 73.0% 4�1, 2, 3

    Note. All tests were Chi-square tests with 3 degrees of freedom, and the significance of all tests was pB0.01; reported significant

    pairwise post-hoc comparisons used an adjusted p-value using the Bonferroni method.

    PTSD, Complex PTSD, and BPD

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097 7
    (page number not for citation purpose)

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    suicidal behaviors while this behavior was not endorsed

    by anyone in the Low Symptoms class and by a relatively

    low and equal proportion in the PTSD and CPTSD

    classes (16.7 and 14.3%, respectively).

    Functional impairment

    Functional impairment was greatest in the BPD (M�
    2.34, SD�0.43) and CPTSD class (M�2.31, SD�0.39)

    relative to the PTSD (M�2.76, SD�0.48) and Low

    Symptom (M�2.71, SD� 0.52) classes. The BPD and

    CPTSD classes did not differ significantly from each other

    in functional impairment (p�0.920). Similarly, the PTSD

    and Low Symptom classes did not differ significantly

    from each other in functional impairment (p�0.983).

    BPD symptoms as indicators of risk for BPD versus

    CPTSD diagnosis

    The salience of each of the BPD symptoms as a ‘‘marker’’

    of being in the BPD class compared to the CPTSD class

    was evaluated. Relative risk (RR) was computed for each

    symptom (see Table 5). RR provides the likelihood that a

    person positive on a particular symptom will be in the

    BPD class relative to the CPTSD class. Each of the BPD

    symptoms was much more likely to be associated with the

    BPD class versus the CPTSD class, except for emptiness.

    The strongest symptom predictors of class were: frantic

    about abandonment, unstable relationships, unstable sense

    of self and impulsiveness.

    Discussion
    Overall, the findings showed that the patterns of symp-

    toms endorsed formed classes that are consistent with

    diagnostic criteria for PTSD, Complex PTSD, and BPD.

    The LCA identified four distinct classes of individuals

    within a treatment-seeking sample: a Low Symptom class

    that was relatively low in all measured symptoms; a

    PTSD class that was high in symptoms of PTSD but

    relatively low in self-organization symptoms and symp-

    toms of BPD; a Complex PTSD class that was high in

    symptoms of PTSD and self-organization symptoms but

    relatively low in symptoms of BPD; and a BPD that was

    high in symptoms of BPD with additional symptoms of

    PTSD and CPTSD. These distinct classes demonstrated

    acceptable discrimination. Additionally, these classes did

    not differ in demographic variables (e.g., age, ethnicity,

    employment status) or total number of traumas experi-

    enced. These findings provide empirical support that the

    symptom profiles endorsed by individuals with Complex

    PTSD and BPD result in distinguishable subgroups of

    trauma-exposed individuals.

    While the individuals in the BPD reported many of

    the symptoms of PTSD and CPTSD, the BPD class was

    clearly distinct in its endorsement of symptoms unique

    to BPD. The RR ratios presented in Table 5 revealed that

    the following symptoms were highly indicative of place-

    ment in the BPD rather than the CPTSD class: (1) frantic

    efforts to avoid real or imagined abandonment, (2) un-

    stable and intense interpersonal relationships characterized

    by alternating between extremes of idealization and

    devaluation, (3) markedly and persistently unstable self-

    image or sense of self, and (4) impulsiveness. Given the

    gravity of suicidal and self-injurious behaviors, it is

    important to note that there were also marked differences

    in the presence of suicidal and self-injurious behaviors

    with approximately 50% of individuals in the BPD class

    reporting this symptom but much fewer and an equivalent

    number doing so in the CPSD and PTSD classes (14.3 and

    16.7%, respectively). The only BPD symptom that in-

    dividuals in the BPD class did not differ from the CPTSD

    class was chronic feelings of emptiness, suggesting that

    in this sample, this symptom is not specific to either BPD

    or CPTSD and does not discriminate between them.

    It should be noted that the endorsement of the CPTSD

    symptoms related to self-organization disturbances was

    high among members of the BPD class. It may be that the

    presence emotion regulation problems does not distin-

    guish CPTSD and BPD, although the severity and type

    might, i.e., suicidality, self-injurious behavior are char-

    acteristic of BPD not CPTSD. Alternatively, it may be that

    the BSI is not optimal as a measure of self-organization

    disturbances to provide differential diagnosis of CPTSD

    versus BPD. The BSI tracks symptoms only for the past 2

    weeks, and thus chronicity of symptoms was not assessed.

    Members of the BPD class may have some but not all of

    the CPTSD symptoms and may vary in their endorsement

    of symptoms across time while the symptoms as endorsed

    by the CPTSD class would be expected to be chronic and

    stable. This interpretation is consistent with the data from

    the SCID-II questions where items highlighting lack of

    stability were strongly endorsed by the BPD but not the

    CPTSD and PTSD class members.

    Table 5. Relative risk of SCID-II BPD items*comparing

    BPD versus CPTSD classes

    BPD symptoms Relative risk 95% CI

    Frantic 2.95* 2.10, 4.15

    Unstable relationships 3.70* 2.18, 6.26

    Unstable sense of self 3.07* 2.14, 4.42

    Impulsiveness 3.04* 2.04, 4.55

    Self-harm 2.10* 1.56, 2.83

    Mood changes 2.04* 1.37, 3.04

    Empty 0.90 0.61, 1.32

    Temper outbursts 2.49* 1.80, 3.45

    Dissociation 2.46* 1.65, 3.68

    CI�Confidence Interval.

    *pB0.01.

    Interpretation example: Individuals positive on the Frantic
    symptom had a 2.95 times greater risk of being in the BPD

    class than those without the Frantic symptom.

    Marylène Cloitre et al.

    8
    (page number not for citation purpose)

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    Overall, the findings indicate that there are several

    ways in which Complex PTSD and BPD differ, consistent

    with the proposed diagnostic formulation of CPTSD.

    BPD is characterized by fears of abandonment, unstable

    sense of self, unstable relationships with others, and impul-

    sive and self-harming behaviors. In contrast, in CPTSD

    as in PTSD, there was little endorsement of items related

    to instability in self-representation or relationships. Self-

    concept is likely to be consistently negative and relational

    difficulties concern mostly avoidance of relationships

    and sense of alienation. Lastly, a comment on the Low

    Symptom class is deserved. The class seems comprised

    of individuals who have very low endorsement of PTSD

    symptoms but somewhat higher endorsements on distur-

    bances in self-organization. These symptoms may reflect

    the presence of subsyndromal BPD or symptoms result-

    ing from a mix other Axis I disorders (Bipolar Disorder,

    Major Depression). Future studies, which evaluate Axis I

    disorders and provide subsyndromal diagnoses, will help

    decipher the nature of this class.

    The distinct symptom profiles characterizing CPTSD

    and BPD lead to different treatment considerations. The

    focus of treatment for BPD concerns reduction of life-

    interfering behaviors such as suicidality and self-injurious

    behaviors, a reduction in dependency on others and an

    increase in an internalized and stable sense of self

    (e.g., Dialectical Behavior Therapy, Linehan, 1993). In

    contrast, treatment programs for CPTSD focus on reduc-

    tion of social and interpersonal avoidance, development of

    a more positive self-concept and relatively rapid engage-

    ment in the review and meaning of traumatic memories

    (e.g., Cloitre et al., 2006). Duration of treatment for each

    disorder and attention to the termination phase are

    different as well. Experts in the treatment of BPD have

    noted that the termination of treatment is a time of risk

    for relapse and symptom exacerbation (see Harned &

    Linehan, 2008). The end of therapy may provoke feelings

    of abandonment, destabilize identity and lead to impul-

    sive and self-injurious behaviors. The DSM guidelines

    for BPD recommend treatment duration of at least 1 year

    (American Psychiatric Association, 2013). A treatment

    course of a year or more may allow for demonstrated

    success in reduction of life-interfering behaviors, the rein-

    forcement and routinization of effective emotion manage-

    ment skills and a carefully planned end to treatment.

    While the recommended duration of treatment for Com-

    plex PTSD has not yet been established, it seems likely be

    shorter than for BPD given the presence of a stable sense

    of self and relative absence of substantial risk for self-

    injurious behaviors and suicidality, but longer than that

    for PTSD, given the greater number and diversity of

    symptoms (see Cloitre et al., 2012).

    Growing attention to patient-centered care, which em-

    phasizes the patient’s specific symptoms, needs and

    preferences will hopefully facilitate the development of

    treatment programming that neither under-treats nor

    over-treats the patient. The proposed spectrum of diag-

    noses moving from PTSD to CPTSD and BPD may

    provide a foundation for developing algorithms of type of

    interventions and duration of care that meets the needs of

    patients with symptom profiles that differ in clinically

    significant ways.

    A number of limitations of the current study are worth

    noting. First, the sample consisted of a treatment-seeking

    sample with a history of childhood interpersonal trauma.

    Replication of results is necessary with samples that are

    more representative of populations in clinical and com-

    munity settings. Future studies should include samples

    with greater diversity in types of trauma as well as

    diversity in the exposure to traumatic stressors. Secondly,

    the data used in the analyses are from a secondary source

    and do not represent the ideal basis for evaluating ICD-11

    PTSD and Complex PTSD symptoms. The Structured

    Interview for Disorders of Extreme Stress (SIDES, Pelcovitz

    et al., 1997), a structured clinician driven measure which

    assesses many of the symptoms of Complex PTSD was

    not available in this data set. Also, the time duration

    for which the symptoms were assessed differed across

    measures and thus did not allow consistency in the

    assessment of the chronicity or variability of the symp-

    toms endorsed. However, the study results, which provide

    evidence of qualitative differences between the CPTSD

    and BPD symptom profiles, suggest the importance of

    developing empirically validated measures of ICD-11

    PTSD and CPTSD and their comparison to BPD in a

    variety of clinical and epidemiological samples.

    Conclusion
    This study identified four distinct classes of individuals

    who have experienced trauma, supporting the proposed

    distinction between Complex PTSD and BPD. Key symp-

    toms that differentiate BPD from Complex PTSD were

    identified. These findings conform to ICD-11’s proposed

    distinction between the diagnoses. They also point to the

    merits of pursuing the construct of CPTSD as a separate

    clinical entity from PTSD and BPD. However, to achieve

    this agenda it is important that empirically validated

    measures of CPTSD be developed for standardized assess-

    ment of the construct in relation to PTSD and BPD.

    Given that that there are efficacious treatments for

    CPTSD (Cloitre et al., 2010) and BPD (e.g., Linehan,

    1993), and these approaches vary in important ways, it is

    useful for clinicians to be able to differentiate between

    these presentations.

    Disclaimer
    M Cloitre and R Bryant are members of the WHO of the

    Working Group on the Classification of Stress-Related

    Disorders. However, the views expressed reflect the opinions

    PTSD, Complex PTSD, and BPD

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097 9
    (page number not for citation purpose)

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    of the authors and not necessarily the Working Group

    and the content of this manuscript does not represent

    WHO policy.

    Conflict of interest and funding

    There is no conflict of interest in the present study for

    any of the authors. This manuscript was supported by

    a National Institute of Mental Health grant, RO1 MH-

    062347 to the first author (M. Cloitre).

    References

    American Psychiatric Association. (2013). Diagnostic and statistical

    manual of mental disorders (5th ed.). Arlington, VA: Author.

    Briere, J., & Rickards, S. (2007). Self-awareness, affect regulation,

    and relatedness: Differential sequels of childhood versus adult

    victimization experiences. Journal of Nervous and Mental

    Disease, 195, 497�503.

    Cloitre, M., Cohen, L., & Koenan, K. (2006). Treating survivors of

    childhood abuse: psychotherapy for the interrupted life. New

    York: Guilford Press.

    Cloitre, M., Courtois, C. A., Ford, J. D., Green, B. L., Alexander, P.,

    Briere, J., et al. (2012). The ISTSS expert consensus treatment

    guidelines for complex PTSD in adults. Retrieved May 13,

    2014, from http://www.istss.org/

    Cloitre, M., Courtois, C. C., Charuvastra, A., Carapezza, R.,

    Stolbach, B. C., & Breen, B. L. (2011). Treatment of complex

    PTSD: Results of the ISTSS expert clinician survey on best

    practices. Journal of Traumatic Stress, 24, 616�627.

    Cloitre, M., Garvert, D. W., Brewin, C. R., Bryant, R. A., &

    Maercker, A. (2013). Evidence for proposed ICD-11 PTSD

    and complex PTSD: A latent profile analysis. European Journal

    of Psychotraumatology, 4, 20706, doi: http://dx.doi.org/10.3402/

    ejpt.v4i0.20706

    Cloitre, M., Stovall-McClough, C. K., Nooner, K., Zorbas, P.,

    Cherry, S., Jackson, C. L., et al. (2010). Treatment of PTSD

    related to childhood abuse: A randomized controlled trial.

    American Journal of Psychiatry, 167, 915�24.

    Derogatis, L. R., & Melisaratos, N. (1983). The brief symptom

    inventory: An introductory report. Psychological Medicine, 13,

    595�605.

    Elklit, A., Hyland, P. & Shevlin, M. (2014). Evidence of symptom

    profiles consistent with posttraumatic stress disorder and

    complex posttraumatic stress disorder in different trauma

    samples. European Journal of Psychotraumatology, 5, 24221,

    doi: http://dx.doi.org/10.3402/ejpt.v5.24221

    First, M. D., Spitzer, M. D., Gibbon, M., & Williams, J. W. (1994).

    Structured Clinical Interview for DSM�IV, Patient Edition.

    New York: Biometrics Research Department, New York State

    Psychiatric Institute.

    Golier, J. A., Yehuda, R., Bierer, L. M., Mitropoulou, V., New, A. S.,

    Schmeidler, J., et al. (2003). The relationship of borderline per-

    sonality disorder to posttraumatic stress disorder and trau-

    matic events. American Journal of Psychiatry, 160, 2018�2024.

    Grant, B. F., Chou, S. P., Goldstein, R. B., Huang, B., Stinson, F. S.,

    Saha, T. D., et al. (2008). Prevalence, correlates, disability,

    and comorbidity of DSM-IV borderline personality disorder:

    Results from the wave 2 national epidemiologic survey on

    alcohol and related conditions. Journal of Clinical Psychiatry,

    69, 533�545.

    Harned, M. S., & Linehan, M. M. (2008). Integrating dialectical

    behavior therapy and prolonged exposure to treat co-occurring

    borderline personality disorder and PTSD: Two case studies.

    Cognitive and Behavioral Practice, 15, 263�276.

    Harned, M. S., Rizvi, S. L., & Linehan, M. M. (2010). The impact of

    co-occurring posttraumatic stress disorder on suicidal women

    with borderline personality disorder. American Journal of

    Psychiatry, 167, 1210�1217.

    Heffernan, K., & Cloitre, M. (2000). A comparison of posttraumatic

    stress disorder with and without borderline personality dis-

    order among women with a history of childhood sexual abuse:

    Etiological and clinical characteristics. Journal of Nervous and

    Mental Disease, 188, 589�595.

    Knefel, M., & Lueger-Schuster, B. (2013). An evaluation of ICD-11

    PTSD and complex PTSD criteria in a sample of adult sur-

    vivors of childhood institutional abuse. European Journal of

    Psychotraumatology, 4, 22608, doi: http://dx.doi.org/10.3402/

    ejpt.v4i0.22608

    Linehan, M. (1993). Cognitive-behavioral treatment of borderline

    personality disorder. New York: Guilford Press.

    Maercker, A., Brewin, C. R., Bryant, R. A., Cloitre, M., Reed, G. M.,

    Van Ommeren, M., et al. (2013). Proposals for mental dis-

    orders specifically associated with stress in the ICD-11. Lancet,

    381(9878), 1683�1685. doi: 10.1016/S0140-6736(12)62191-6.

    Nylund, K. L., Asparouhov, T., & Muthen, B. O. (2007). Deciding

    on the number of classes in latent class analysis and growth

    mixture modelling: A Monte Carlo simulation study. Struc-

    tural Equation Modeling, 14(4), 535�569.

    Pagura, J., Stein, M. B., Bolton, J. M., Cox, B. J., Grant, B., &

    Sareen, J. (2010). Comorbidity of borderline personality dis-

    order and posttraumatic stress disorder in the U.S. population.

    Journal of Psychiatric Research, 44, 1190�1198.

    Pelcovitz, D., Van der Kolk, B., Roth, S., Mandel, F., Kaplan, S., &

    Resick, P. (1997). Development of a criteria set and a struc-

    tured interview for disorders of extreme stress (SIDES).

    Journal of Traumatic Stress, 10, 3�17.

    Reed, G. M. (2010). Toward ICD-11: Improving the clinical utility

    of WHO’s international classification of mental disorders.

    Professional Psychology: Research and Practice, 41, 457�464.

    Weathers, F. W., Keane, T. M., & Davidson, J. R. T. (2001).

    Clinician-Administered PTSD Scale: A review of the first ten

    years of research. Depression and Anxiety, 13, 132�156.

    Weissman, E., & Bothell, S. (1976). Assessment of patient social

    adjustment by patient self-report. Archives of General Psychia-

    try, 33, 1111�1115.

    Zanarini, M. C., Frankenburg, F. R., Dubo, E. D., Sickel, A.,

    Trikha, A., Levin, A., et al. (1998). Axis I comorbidity of border-

    line personality disorder. American Journal of Psychiatry, 155,

    1733�1739.

    Zlotnick, C., Franklin, C. L., & Zimmerman, M. (2002). Is

    comorbidity of posttraumatic stress disorder and borderline

    personality disorder related to greater pathology and impair-

    ment? American Journal of Psychiatry, 159, 1940�1043.

    Marylène Cloitre et al.

    10
    (page number not for citation purpose)

    Citation: European Journal of Psychotraumatology 2014, 5: 25097 – http://dx.doi.org/10.3402/ejpt.v5.25097

    http://www.istss.org/

    http://dx.doi.org/10.3402/ejpt.v4i0.20706

    http://dx.doi.org/10.3402/ejpt.v4i0.20706

    http://dx.doi.org/10.3402/ejpt.v5.24221

    http://dx.doi.org/10.3402/ejpt.v4i0.22608

    http://dx.doi.org/10.3402/ejpt.v4i0.22608

    http://eurojnlofpsychotraumatol.net/index.php/ejpt/article/view/25097

    http://dx.doi.org/10.3402/ejpt.v5.25097

    << /ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /None /Binding /Left /CalGrayProfile (Dot Gain 30%) /CalRGBProfile (None) /CalCMYKProfile (U.S. Sheetfed Coated v2) /sRGBProfile (sRGB IEC61966-2.1) /CannotEmbedFontPolicy /Error /CompatibilityLevel 1.4 /CompressObjects /Off /CompressPages true /ConvertImagesToIndexed false /PassThroughJPEGImages true /CreateJobTicket false /DefaultRenderingIntent /Default /DetectBlends true /DetectCurves 0.1000 /ColorConversionStrategy /LeaveColorUnchanged /DoThumbnails false /EmbedAllFonts true /EmbedOpenType false /ParseICCProfilesInComments true /EmbedJobOptions true /DSCReportingLevel 0 /EmitDSCWarnings false /EndPage -1 /ImageMemory 1048576 /LockDistillerParams false /MaxSubsetPct 100 /Optimize false /OPM 1 /ParseDSCComments true /ParseDSCCommentsForDocInfo true /PreserveCopyPage true /PreserveDICMYKValues true /PreserveEPSInfo true /PreserveFlatness true /PreserveHalftoneInfo false /PreserveOPIComments false /PreserveOverprintSettings true /StartPage 1 /SubsetFonts true /TransferFunctionInfo /Apply /UCRandBGInfo /Remove /UsePrologue false /ColorSettingsFile (None) /AlwaysEmbed [ true ] /NeverEmbed [ true ] /AntiAliasColorImages false /CropColorImages true /ColorImageMinResolution 150 /ColorImageMinResolutionPolicy /OK /DownsampleColorImages false /ColorImageDownsampleType /Average /ColorImageResolution 300 /ColorImageDepth -1 /ColorImageMinDownsampleDepth 1 /ColorImageDownsampleThreshold 1.50000 /EncodeColorImages true /ColorImageFilter /DCTEncode /AutoFilterColorImages false /ColorImageAutoFilterStrategy /JPEG /ColorACSImageDict << /QFactor 0.15 /HSamples [1 1 1 1] /VSamples [1 1 1 1] >>
    /ColorImageDict << /QFactor 0.40 /HSamples [1 1 1 1] /VSamples [1 1 1 1] >>
    /JPEG2000ColorACSImageDict << /TileWidth 256 /TileHeight 256 /Quality 30 >>
    /JPEG2000ColorImageDict << /TileWidth 256 /TileHeight 256 /Quality 30 >>
    /AntiAliasGrayImages false
    /CropGrayImages true
    /GrayImageMinResolution 150
    /GrayImageMinResolutionPolicy /OK
    /DownsampleGrayImages false
    /GrayImageDownsampleType /Average
    /GrayImageResolution 300
    /GrayImageDepth -1
    /GrayImageMinDownsampleDepth 2
    /GrayImageDownsampleThreshold 1.50000
    /EncodeGrayImages true
    /GrayImageFilter /DCTEncode
    /AutoFilterGrayImages false
    /GrayImageAutoFilterStrategy /JPEG
    /GrayACSImageDict << /QFactor 0.15 /HSamples [1 1 1 1] /VSamples [1 1 1 1] >>
    /GrayImageDict << /QFactor 0.40 /HSamples [1 1 1 1] /VSamples [1 1 1 1] >>
    /JPEG2000GrayACSImageDict << /TileWidth 256 /TileHeight 256 /Quality 30 >>
    /JPEG2000GrayImageDict << /TileWidth 256 /TileHeight 256 /Quality 30 >>
    /AntiAliasMonoImages false
    /CropMonoImages true
    /MonoImageMinResolution 1200
    /MonoImageMinResolutionPolicy /OK
    /DownsampleMonoImages false
    /MonoImageDownsampleType /Average
    /MonoImageResolution 1200
    /MonoImageDepth -1
    /MonoImageDownsampleThreshold 1.50000
    /EncodeMonoImages true
    /MonoImageFilter /CCITTFaxEncode
    /MonoImageDict << /K -1 >>
    /AllowPSXObjects false
    /CheckCompliance [
    /None
    ]
    /PDFX1aCheck false
    /PDFX3Check false
    /PDFXCompliantPDFOnly true
    /PDFXNoTrimBoxError false
    /PDFXTrimBoxToMediaBoxOffset [
    0.00000
    0.00000
    0.00000
    0.00000
    ]
    /PDFXSetBleedBoxToMediaBox false
    /PDFXBleedBoxToTrimBoxOffset [
    0.00000
    0.00000
    0.00000
    0.00000
    ]
    /PDFXOutputIntentProfile (Euroscale Coated v2)
    /PDFXOutputConditionIdentifier ()
    /PDFXOutputCondition ()
    /PDFXRegistryName (http://www.color.org)
    /PDFXTrapped /False
    /CreateJDFFile false
    /SyntheticBoldness 1.000000
    /Description << /DEU
    /FRA
    /JPN
    /PTB
    /DAN
    /NLD
    /ESP
    /SUO
    /ITA
    /NOR
    /SVE
    /ENU
    >>
    >> setdistillerparams
    << /HWResolution [2400 2400] /PageSize [612.000 792.000] >> setpagedevice

    Research Article

    Relationship of Childhood Abuse and
    Household Dysfunction to Many of the
    Leading Causes of Death in Adult

    s

    The Adverse Childhood Experiences (ACE) Stud

    y

    Vincent J. Felitti, MD, FACP, Robert F. Anda, MD, MS, Dale Nordenberg, MD, David F. Williamson, MS, PhD,
    Alison M. Spitz, MS, MPH, Valerie Edwards, BA, Mary P. Koss, PhD, James S. Marks, MD, MPH

    Background: The relationship of health risk behavior and disease in adulthood to the breadth of
    exposure to childhood emotional, physical, or sexual abuse, and household dysfunction
    during childhood has not previously been described.

    Methods: A questionnaire about adverse childhood experiences was mailed to 13,494 adults who had
    completed a standardized medical evaluation at a large HMO; 9,508 (70.5%) responded.
    Seven categories of adverse childhood experiences were studied: psychological, physical, or
    sexual abuse; violence against mother; or living with household members who were
    substance abusers, mentally ill or suicidal, or ever imprisoned. The number of categories
    of these adverse childhood experiences was then compared to measures of adult risk
    behavior, health status, and disease. Logistic regression was used to adjust for effects of
    demographic factors on the association between the cumulative number of categories of
    childhood exposures (range: 0–7) and risk factors for the leading causes of death in adult
    life.

    Results: More than half of respondents reported at least one, and one-fourth reported $2
    categories of childhood exposures. We found a graded relationship between the number
    of categories of childhood exposure and each of the adult health risk behaviors and
    diseases that were studied (P , .001). Persons who had experienced four or more
    categories of childhood exposure, compared to those who had experienced none, had 4-
    to 12-fold increased health risks for alcoholism, drug abuse, depression, and suicide
    attempt; a 2- to 4-fold increase in smoking, poor self-rated health, $50 sexual intercourse
    partners, and sexually transmitted disease; and a 1.4- to 1.6-fold increase in physical
    inactivity and severe obesity. The number of categories of adverse childhood exposures
    showed a graded relationship to the presence of adult diseases including ischemic heart
    disease, cancer, chronic lung disease, skeletal fractures, and liver disease. The seven
    categories of adverse childhood experiences were strongly interrelated and persons with
    multiple categories of childhood exposure were likely to have multiple health risk factors
    later in life.

    Conclusions: We found a strong graded relationship between the breadth of exposure to abuse or
    household dysfunction during childhood and multiple risk factors for several of the
    leading causes of death in adults.

    Medical Subject Headings (MeSH): child abuse, sexual, domestic violence, spouse abuse,
    children of impaired parents, substance abuse, alcoholism, smoking, obesity, physical
    activity, depression, suicide, sexual behavior, sexually transmitted diseases, chronic obstruc-
    tive pulmonary disease, ischemic heart disease. (Am J Prev Med 1998;14:245–258) © 1998
    American Journal of Preventive Medicine

    Department of Preventive Medicine, Southern California Perma-
    nente Medical Group (Kaiser Permanente), (Felitti) San Diego,
    California 92111. National Center for Chronic Disease Prevention
    and Health Promotion, Centers for Disease Control and Prevention,
    (Anda, Williamson, Spitz, Edwards, Marks) Atlanta, Georgia 30333.
    Department of Pediatrics, Emory University School Medicine, (Nor–

    denberg) Atlanta, Georgia 30333. Department of Family and Com-
    munity Medicine, University of Arizona Health Sciences Center,
    (Koss) Tucson, Arizona 85727.

    Address correspondence to: Vincent J. Felitti, MD, Kaiser Perma-
    nente, Department of Preventive Medicine, 7060 Clairemont Mesa
    Boulevard, San Diego, California 92111.

    245Am J Prev Med 1998;14(4) 0749-3797/98/$19.00
    © 1998 American Journal of Preventive Medicine PII S0749-3797(98)00017-8

    Introduction

    Only recently have medical investigators in pri-
    mary care settings begun to examine associa-
    tions between childhood abuse and adult

    health risk behaviors and disease.1–5 These associations
    are important because it is now clear that the leading
    causes of morbidity and mortality in the United States6

    are related to health behaviors and lifestyle factors;
    these factors have been called the “actual” causes of
    death.7 Insofar as abuse and other potentially
    damaging childhood experiences contribute
    to the development of these risk factors, then
    these childhood exposures should be recog-
    nized as the basic causes of morbidity and
    mortality in adult life.

    Although sociologists and psychologists
    have published numerous articles about the
    frequency8–12 and long-term consequenc-
    es13–15 of childhood abuse, understanding their rele-
    vance to adult medical problems is rudimentary. Fur-
    thermore, medical research in this field has limited
    relevance to most primary care physicians because it is
    focused on adolescent health,16–20 mental health in
    adults,20 or on symptoms among patients in specialty
    clinics.22,23 Studies of the long-term effects of child-
    hood abuse have usually examined single types of abuse,
    particularly sexual abuse, and few have assessed the im-
    pact of more than one type of abuse.5,24–28 Conditions
    such as drug abuse, spousal violence, and criminal activity
    in the household may co-occur with specific forms of
    abuse that involve children. Without measuring these
    household factors as well, long-term influence might be
    wrongly attributed solely to single types of abuse and
    the cumulative influence of multiple categories of
    adverse childhood experiences would not be assessed.
    To our knowledge, the relationship of adult health risk
    behaviors, health status, and disease states to childhood
    abuse and household dysfunction29–35 has not been
    described.

    We undertook the Adverse Childhood Experiences
    (ACE) Study in a primary care setting to describe the
    long-term relationship of childhood experiences to
    important medical and public health problems. The
    ACE Study is assessing, retrospectively and prospec-
    tively, the long-term impact of abuse and household
    dysfunction during childhood on the following out-
    comes in adults: disease risk factors and incidence,
    quality of life, health care utilization, and mortality. In
    this initial paper we use baseline data from the study to
    provide an overview of the prevalence and interrelation
    of exposures to childhood abuse and household dys-
    function. We then describe the relationship between
    the number of categories of these deleterious child-
    hood exposures and risk factors and those diseases that

    underlie many of the leading causes of death in
    adults.6,7,36,37

    Methods
    Study Setting

    The ACE Study is based at Kaiser Permanente’s San
    Diego Health Appraisal Clinic. More than 45,000 adults
    undergo standardized examinations there each year,
    making this clinic one of the nation’s largest free-

    standing medical evaluation centers. All en-
    rollees in the Kaiser Health Plan in San
    Diego are advised through sales literature
    about the services (free for members) at the
    clinic; after enrollment, members are ad-
    vised again of its availability through new-
    member literature. Most members obtain
    appointments by self-referral; 20% are re-

    ferred by their health care provider. A recent review of
    membership and utilization records among Kaiser
    members in San Diego continuously enrolled between
    1992 and 1995 showed that 81% of those 25 years and
    older had been evaluated in the Health Appraisal
    Clinic.

    Health appraisals include completion of a standard-
    ized medical questionnaire that requests demographic
    and biopsychosocial information, review of organ sys-
    tems, previous medical diagnoses, and family medical
    history. A health care provider completes the medical
    history, performs a physical examination, and reviews
    the results of laboratory tests with the patient.

    Survey Methods

    The ACE Study protocol was approved by the Institu-
    tional Review Boards of the Southern California Per-
    manente Medical Group (Kaiser Permanente), the
    Emory University School of Medicine, and the Office of
    Protection from Research Risks, National Institutes of
    Health. All 13,494 Kaiser Health Plan members who
    completed standardized medical evaluations at the
    Health Appraisal Clinic between August–November of
    1995 and January–March of 1996 were eligible to
    participate in the ACE Study. Those seen at the clinic
    during December were not included because survey
    response rates are known to be lower during the
    holiday period.38

    In the week after visiting the clinic, and hence
    having their standardized medical history already
    completed, members were mailed the ACE Study
    questionnaire that included questions about child-
    hood abuse and exposure to forms of household
    dysfunction while growing up. After second mailings
    of the questionnaire to persons who did not respond
    to the first mailing, the response rate for the survey
    was 70.5% (9,508/13,494).

    See
    related

    Commentary
    on pages 354,

    356, 361.

    246 American Journal of Preventive Medicine, Volume 14, Number 4

    A second survey wave of approximately the same
    number of patients as the first wave was conducted
    between June and October of 1997. The data for the
    second survey wave is currently being compiled for
    analysis. The methods for the second mail survey wave
    were identical to the first survey wave as described
    above. The second wave was done to enhance the
    precision of future detailed analyses on special topics
    and to reduce the time necessary to obtain precise
    statistics on follow-up health events. An overview of the
    total ACE Study design is provided in Figure 1.

    Comparison of
    Respondents and Nonrespondents

    We abstracted the completed medical evaluation for
    every person eligible for the study; this included their
    medical history, laboratory results, and physical find-
    ings. Respondent (n 5 9,508) and nonrespondent
    (n 5 3,986) groups were similar in their percentages
    of women (53.7% and 51.0%, respectively) and in their
    mean years of education (14.0 years and 13.6 years,
    respectively). Respondents were older than nonrespon-
    dents (means 56.1 years and 49.3 years) and more likely
    to be white (83.9% vs. 75.3%) although the actual
    magnitude of the differences was small.

    Respondents and nonrespondents did not differ with
    regard to their self-rated health, smoking, other sub-
    stance abuse, or the presence of common medical
    conditions such as a history of heart attack or stroke,
    chronic obstructive lung disease, hypertension, or dia-
    betes, or with regard to marital status or current family,
    marital, or job-related problems (data not shown). The
    health appraisal questionnaire used in the clinic con-

    tains a single question about childhood sexual abuse
    that reads “As a child were you ever raped or sexually
    molested?” Respondents were slightly more likely to
    answer affirmatively than nonrespondents (6.1% vs.
    5.4%, respectively).

    Questionnaire Design

    We used questions from published surveys to construct
    the ACE Study questionnaire. Questions from the Con-
    flicts Tactics Scale39 were used to define psychological
    and physical abuse during childhood and to define
    violence against the respondent’s mother. We adapted
    four questions from Wyatt40 to define contact sexual
    abuse during childhood. Questions about exposure to
    alcohol or drug abuse during childhood were adapted
    from the 1988 National Health Interview Survey.41 All
    of the questions we used in this study to determine
    childhood experiences were introduced with the
    phrase “While you were growing up during your first 18
    years of life . . .”

    Questions about health-related behaviors and health
    problems were taken from health surveys such as the
    Behavioral Risk Factor Surveys42 and the Third Na-
    tional Health and Nutrition Examination Survey,43

    both of which are directed by the Centers for Disease
    Control and Prevention. Questions about depression
    came from the Diagnostic Interview Schedule of the
    National Institute of Mental Health (NIMH).44 Other
    information for this analysis such as disease history was
    obtained from the standardized questionnaire used in
    the Health Appraisal Clinic. (A copy of the question-
    naires used in this study may be found at www.elsevier.
    com/locate/amepre.)

    Figure 1. ACE Study design. *After exclusions, 59.7% of the original wave I sample (8,056/13,494) were included in this analysis.

    Am J Prev Med 1998;14(4) 247

    Defining Childhood Exposures

    We used three categories of childhood abuse: psycho-
    logical abuse (2 questions), physical abuse (2 ques-
    tions), or contact sexual abuse (4 questions). There
    were four categories of exposure to household dysfunc-
    tion during childhood: exposure to substance abuse
    (defined by 2 questions), mental illness (2 questions),
    violent treatment of mother or stepmother (4 ques-
    tions), and criminal behavior (1 question) in the house-
    hold. Respondents were defined as exposed to a cate-
    gory if they responded “yes” to 1 or more of the
    questions in that category. The prevalence of positive
    responses to the individual questions and the category
    prevalences are shown in Table 1.

    We used these 7 categories of childhood exposures to
    abuse and household dysfunction for our analysis. The
    measure of childhood exposure that we used was simply
    the sum of the categories with an exposure; thus the
    possible number of exposures ranged from 0 (unex-
    posed) to 7 (exposed to all categories).

    Risk Factors and Disease Conditions Assessed

    Using information from both the study questionnaire
    and the Health Appraisal Clinic’s questionnaire, we
    chose 10 risk factors that contribute to the leading
    causes of morbidity and mortality in the United
    States.6,7,36,37 The risk factors included smoking, severe
    obesity, physical inactivity, depressed mood, suicide
    attempts, alcoholism, any drug abuse, parenteral drug
    abuse, a high lifetime number of sexual partners
    ($50), and a history of having a sexually transmitted
    disease.

    We also assessed the relationship between childhood
    exposures and disease conditions that are among the
    leading causes of mortality in the United States.6 The
    presence of these disease conditions was based upon
    medical histories that patients provided in response to
    the clinic questionnaire. We included a history of
    ischemic heart disease (including heart attack or use of
    nitroglycerin for exertional chest pain), any cancer,
    stroke, chronic bronchitis, or emphysema (COPD),

    Table 1. Prevalence of childhood exposure to abuse and household dysfunction

    Category of childhood exposurea Prevalence (%) Prevalence (%)

    Abuse by category
    Psychological 11.1

    (Did a parent or other adult in the household . . .)
    Often or very often swear at, insult, or put you down? 10.0
    Often or very often act in a way that made you afraid that

    you would be physically hurt?
    4.8

    Physical 10.8
    (Did a parent or other adult in the household . . .)

    Often or very often push, grab, shove, or slap you? 4.9
    Often or very often hit you so hard that you had marks or

    were injured?
    9.6

    Sexual 22.0
    (Did an adult or person at least 5 years older ever . . .)

    Touch or fondle you in a sexual way? 19.3
    Have you touch their body in a sexual way? 8.7
    Attempt oral, anal, or vaginal intercourse with you? 8.9
    Actually have oral, anal, or vaginal intercourse with you? 6.9

    Household dysfunction by category
    Substance abuse 25.6

    Live with anyone who was a problem drinker or alcoholic? 23.5
    Live with anyone who used street drugs? 4.9

    Mental illness 18.8
    Was a household member depressed or mentally ill? 17.5
    Did a household member attempt suicide? 4.0

    Mother treated violently 12.5
    Was your mother (or stepmother)

    Sometimes, often, or very often pushed, grabbed, slapped,
    or had something thrown at her?

    11.9

    Sometimes, often, or very often kicked, bitten, hit with a
    fist, or hit with something hard?

    6.3

    Ever repeatedly hit over at least a few minutes? 6.6
    Ever threatened with, or hurt by, a knife or gun? 3.0

    Criminal behavior in household
    Did a household member go to prison? 3.4 3.4

    Any category reported 52.1%
    aAn exposure to one or more items listed under the set of questions for each category.

    248 American Journal of Preventive Medicine, Volume 14, Number 4

    diabetes, hepatitis or jaundice, and any skeletal frac-
    tures (as a proxy for risk of unintentional injuries). We
    also included responses to the following question about
    self-rated health: “Do you consider your physical health
    to be excellent, very good, good, fair, or poor?” because
    it is strongly predictive of mortality.45

    Definition of Risk Factors

    We defined severe obesity as a body mass index (kg/
    meter2) $35 based on measured height and weight;
    physical inactivity as no participation in recreational
    physical activity in the past month; and alcoholism as a
    “Yes” response to the question “Have you ever consid-
    ered yourself to be an alcoholic?” The other risk factors
    that we assessed are self-explanatory.

    Exclusions from Analysis

    Of the 9,508 survey respondents, we excluded 51
    (0.5%) whose race was unstated and 34 (0.4%) whose
    educational attainment was not reported. We also ex-
    cluded persons who did not respond to certain ques-
    tions about adverse childhood experiences. This in-
    volved the following exclusions: 125 (1.3%) for
    household substance abuse, 181 (1.9%) for mental
    illness in the home, 148 (1.6%) for violence against
    mother, 7 (0.1%) for imprisonment of a household
    member, 109 (1.1%) for childhood psychological
    abuse, 44 (0.5%) for childhood physical abuse, and 753
    (7.9%) for childhood sexual abuse. After these exclu-
    sions, 8,056 of the original 9,508 survey respondents
    (59.7% of the original sample of 13,494) remained and
    were included in the analysis. Procedures for insuring
    that the findings based on complete data were gener-
    alizable to the entire sample are described below.

    The mean age of the 8,506 persons included in this
    analysis was 56.1 years (range: 19–92 years); 52.1% were
    women; 79.4% were white. Forty-three percent had
    graduated from college; only 6.0% had not graduated
    from high school.

    Statistical Analysis

    We used the Statistical Analysis System (SAS)46 for our
    analyses. We used the direct method to age-adjust the
    prevalence estimates. Logistic regression analysis was
    employed to adjust for the potential confounding ef-
    fects of age, sex, race, and educational attainment on
    the relationship between the number of childhood
    exposures and health problems.

    To test for a dose-response relationship to health
    problems, we entered the number of childhood expo-
    sures as a single ordinal variable (0, 1, 2, 3, 4, 5, 6, 7)
    into a separate logistic regression model for each risk
    factor or disease condition.

    Assessing the Possible Influence of Exclusions

    To determine whether our results were influenced by
    excluding persons with incomplete information on any
    of the categories of childhood exposure, we performed
    a separate sensitivity analysis in which we included all
    persons with complete demographic information but
    assumed that persons with missing information for a
    category of childhood exposure did not have an expo-
    sure in that category.

    Results
    Adverse Childhood Exposures

    The level of positive responses for the 17 questions
    included in the seven categories of childhood exposure
    ranged from 3.0% for a respondent’s mother (or
    stepmother) having been threatened with or hurt by a
    gun or knife to 23.5% for having lived with a problem
    drinker or alcoholic (Table 1). The most prevalent of
    the 7 categories of childhood exposure was substance
    abuse in the household (25.6%); the least prevalent
    exposure category was evidence of criminal behavior in
    the household (3.4%). More than half of respondents
    (52%) experienced $1 category of adverse childhood
    exposure; 6.2% reported $4 exposures.

    Relationships between
    Categories of Childhood Exposure

    The probability that persons who were exposed to any
    single category of exposure were also exposed to an-
    other category is shown in Table 2. The relationship
    between single categories of exposure was significant
    for all comparisons (P , .001; chi-square). For persons
    reporting any single category of exposure, the proba-
    bility of exposure to any additional category ranged
    from 65%–93% (median: 80%); similarly, the probabil-
    ity of $2 additional exposures ranged from 40%–74%
    (median: 54.5%).

    The number of categories of childhood exposures by
    demographic characteristics is shown in Table 3. Statis-
    tically, significantly fewer categories of exposure were
    found among older persons, white or Asian persons,
    and college graduates (P , .001). Because age is
    associated with both the childhood exposures as well as
    many of the health risk factors and disease outcomes,
    all prevalence estimates in the tables are adjusted for
    age.

    Relationship between
    Childhood Exposures and Health Risk Factors

    Both the prevalence and risk (adjusted odds ratio)
    increased for smoking, severe obesity, physical inactiv-
    ity, depressed mood, and suicide attempts as the num-
    ber of childhood exposures increased (Table 4). When

    Am J Prev Med 1998;14(4) 249

    persons with 4 categories of exposure were compared
    to those with none, the odds ratios ranged from 1.3 for
    physical inactivity to 12.2 for suicide attempts (Table 4).

    Similarly, the prevalence and risk (adjusted odds
    ratio) of alcoholism, use of illicit drugs, injection of
    illicit drugs, $50 intercourse partners, and history of a
    sexually transmitted disease increased as the number of
    childhood exposures increased (Table 5). In compar-
    ing persons with $4 childhood exposures to those with
    none, odds ratios ranged from 2.5 for sexually trans-
    mitted diseases to 7.4 for alcoholism and 10.3 for
    injected drug use.

    Childhood Exposures and
    Clustering of Health Risk Factors

    We found a strong relationship between the number of
    childhood exposures and the number of health risk
    factors for leading causes of death in adults (Table 6).
    For example, among persons with no childhood expo-
    sures, 56% had none of the 10 risk factors whereas only
    14% of persons with $4 categories of childhood expo-
    sure had no risk factors. By contrast, only 1% of persons
    with no childhood exposures had four or more risk
    factors, whereas 7% of persons with $4 childhood
    exposures had four or more risk factors (Table 6).

    Relationship between
    Childhood Exposures and Disease Conditions

    When persons with 4 or more categories of childhood
    exposure were compared to those with none, the
    odds ratios for the presence of studied disease con-
    ditions ranged from 1.6 for diabetes to 3.9 for
    chronic bronchitis or emphysema (Table 7). Simi-
    larly, the odds ratios for skeletal fractures, hepatitis
    or jaundice, and poor self-rated health were 1.6, 2.3,
    and 2.2, respectively (Table 8).

    Significance of Dose-Response Relationships

    In logistic regression models (which included age,
    gender, race, and educational attainment as covariates)
    we found a strong, dose-response relationship between
    the number of childhood exposures and each of the 10
    risk factors for the leading causes of death that we
    studied (P , .001). We also found a significant (P ,
    .05) dose-response relationship between the number
    of childhood exposures and the following disease con-
    ditions: ischemic heart disease, cancer, chronic bron-
    chitis or emphysema, history of hepatitis or jaundice,
    skeletal fractures, and poor self-rated health. There was
    no statistically significant dose-response relationship
    for a history of stroke or diabetes.T

    ab
    le

    2.
    R

    el
    at

    io
    n

    sh
    ip

    s
    be

    tw
    ee

    n
    ca

    te
    go

    ri
    es

    of
    ad

    ve
    rs

    e
    ch

    ild
    h

    oo
    d

    ex
    po

    su
    re

    P
    er

    ce
    nt

    (%
    )

    E
    xp

    os
    ed

    t

    o
    A

    no
    th

    er
    C

    at
    eg

    or
    y

    Fi
    rs

    t
    C

    at
    eg

    or
    y

    of
    C

    hi
    ld

    ho
    od

    E
    xp

    os
    ur

    e
    Sa

    m
    pl

    e
    Si

    ze
    *

    P
    sy

    c

    h
    ol

    og
    ic

    a

    l
    A

    bu
    se

    P
    hy

    si
    ca

    l
    A

    bu
    se

    Se
    xu

    al
    A

    bu
    se

    Su
    bs

    ta
    nc

    e
    A

    bu
    se

    M
    en

    ta
    l

    Il
    ln

    es
    s

    T
    re

    at
    ed

    V
    io

    le
    nt

    ly
    Im

    pr
    is

    on
    ed

    M
    em

    be
    r

    A
    ny

    O
    ne

    A
    dd

    it
    io

    na
    l

    C
    at

    eg
    or

    y

    A
    ny

    T
    w

    o
    A

    dd
    it

    io
    na

    l
    C

    at
    eg

    or
    ie

    s

    C
    h

    ild
    h

    oo
    d

    A
    bu

    se
    :

    Ps
    yc

    h
    ol

    og
    ic

    al
    89

    8

    52
    *

    47
    51

    50
    39

    9
    93

    74
    Ph

    ys
    ic

    al
    ab

    us
    e

    87
    4

    54

    44
    45

    38
    35

    9
    86

    64
    Se

    xu
    al

    ab
    us

    e
    17

    70
    24

    22

    39
    31

    23
    6

    65
    41

    H
    ou

    se
    h

    ol
    d

    dy
    sf

    un
    ct

    io
    n

    :
    Su

    bs
    ta

    n
    ce

    ab
    us

    e
    20

    64
    22

    19
    34


    34

    29
    8

    69
    40

    M
    en

    ta
    l

    ill
    n

    es
    s

    15
    12

    30
    22

    37
    46


    26

    7
    74

    47
    M

    ot
    h

    er
    tr

    ea
    te

    d
    vi

    ol
    en

    tl
    y

    10
    10

    34
    31

    41
    59

    38

    10
    86

    62
    M

    em
    be

    r
    im

    pr
    is

    on
    ed

    27
    1

    29
    29

    40
    62

    42
    37


    86

    64
    m

    ed
    ia

    n
    29

    .5
    25

    .4
    40

    .5
    48

    .5
    38

    32
    8.

    5
    80

    54
    .5

    ra
    n

    ge
    (2

    2–
    54

    )
    (1

    9–
    52

    )
    (3

    4–
    47

    )
    (3

    9–
    62

    )
    (3

    1–
    50

    )
    (2

    3–
    39

    )
    (6

    –1
    0)

    (6
    5–

    93
    )

    (4
    0–

    74
    )

    *N
    um

    be
    r

    ex
    po

    se
    d

    to
    fi

    rs
    t

    ca
    te

    go
    ry

    .
    Fo

    r
    ex

    am
    pl

    e,
    am

    on
    g

    pe
    rs

    on
    s

    w
    h

    o
    w

    er
    e

    ps
    yc

    h
    ol

    og
    ic

    al
    ly

    ab
    us

    ed
    ,

    52
    %

    w
    er

    e
    al

    so
    ph

    ys
    ic

    al
    ly

    ab
    us

    ed
    .

    M
    or

    e
    pe

    rs
    on

    s
    w

    er
    e

    a
    se

    co
    n

    d
    ca

    te
    go

    ry
    th

    an
    w

    ou
    ld

    be
    ex

    pe
    ct

    ed
    by

    ch
    an

    ce
    (P

    ,
    .0

    01
    ;

    ch
    i-s

    qu
    ar

    e)
    .

    250 American Journal of Preventive Medicine, Volume 14, Number 4

    Assessment of the Influence of Exclusions

    In the sensitivity analysis where missing information for
    a category of childhood exposure was considered as no
    exposure, the direction and strength of the associations
    between the number of childhood exposures and the
    risk factors and disease conditions were nearly identical
    (data not shown). Thus, the results we present appear
    to be unaffected by our decision to exclude persons for
    whom information on any category of childhood expo-
    sure was incomplete.

    Discussion

    We found a strong dose response relationship between
    the breadth of exposure to abuse or household dysfunc-
    tion during childhood and multiple risk factors for
    several of the leading causes of death in adults. Disease
    conditions including ischemic heart disease, cancer,
    chronic lung disease, skeletal fractures, and liver dis-
    ease, as well as poor self-rated health also showed a
    graded relationship to the breadth of childhood expo-
    sures. The findings suggest that the impact of these
    adverse childhood experiences on adult health status is
    strong and cumulative.

    The clear majority of patients in our study who were
    exposed to one category of childhood abuse or house-
    hold dysfunction were also exposed to at least one
    other. Therefore, researchers trying to understand the
    long-term health implications of childhood abuse may
    benefit from considering a wide range of related ad-
    verse childhood exposures. Certain adult health out-

    comes may be more strongly related to unique combi-
    nations or the intensity of adverse childhood exposures
    than to the total breadth of exposure that we used for
    our analysis. However, the analysis we present illustrates
    the need for an overview of the net effects of a group of
    complex interactions on a wide range of health risk
    behaviors and diseases.

    Several potential limitations need to be considered
    when interpreting the results of this study. The data
    about adverse childhood experiences are based on
    self-report, retrospective, and can only demonstrate
    associations between childhood exposures and health
    risk behaviors, health status, and diseases in adulthood.
    Second, some persons with health risk behaviors or
    diseases may have been either more, or less, likely to
    report adverse childhood experiences. Each of these
    issues potentially limits inferences about causality. Fur-
    thermore, disease conditions could be either over- or
    under-reported by patients when they complete the
    medical questionnaire. In addition, there may be me-
    diators of the relationship between childhood experi-
    ences and adult health status other than the risk factors
    we examined. For example, adverse childhood experi-
    ences may affect attitudes and behaviors toward health
    and health care, sensitivity to internal sensations, or
    physiologic functioning in brain centers and neuro-
    transmitter systems. A more complete understanding
    of these issues is likely to lead to more effective ways
    to address the long-term health problems associated
    with childhood abuse and household dysfunction.

    However, our estimates of the prevalence of child-

    Table 3. Prevalence of categories of adverse childhood exposures by demographic characteristics

    Characteristic
    Sample size
    (N)

    Number of categories (%)a

    0 1 2 3 4

    Age group (years)
    19–34 807 35.4 25.4 17.2 11.0 10.9
    35–49 2,063 39.3 25.1 15.6 9.1 10.9
    50–64 2,577 46.5 25.2 13.9 7.9 6.6
    $65 2,610 60.0 24.5 8.9 4.2 2.4

    Genderb

    Women 4,197 45.4 24.0 13.4 8.7 8.5
    Men 3,859 53.7 25.8 11.6 5.0 3.9

    Raceb

    White 6,432 49.7 25.3 12.4 6.7 6.0
    Black 385 38.8 25.7 16.3 12.3 7.0
    Hispanic 431 42.9 24.9 13.7 7.4 11.2
    Asian 508 66.0 19.0 9.9 3.4 1.7
    Other 300 41.0 23.5 13.9 9.5 12.1

    Educationb

    No HS diploma 480 56.5 21.5 8.4 6.5 7.2
    HS graduate 1,536 51.6 24.5 11.3 7.4 5.2
    Any college 2,541 44.1 25.5 14.8 7.8 7.8
    College graduate 3,499 51.4 25.1 12.1 6.1 5.3
    All participants 8,056 49.5 24.9 12.5 6.9 6.2

    aThe number of categories of exposure was simply the sum of each of the seven individual categories that were assessed (see Table 1).
    bPrevalence estimates adjusted for age.

    Am J Prev Med 1998;14(4) 251

    hood exposures are similar to estimates from nationally
    representative surveys, indicating that the experiences
    of our study participants are comparable to the larger
    population of U.S. adults. In our study, 23.5% of
    participants reported having grown up with an alcohol
    abuser; the 1988 National Health Interview Survey
    estimated that 18.1% of adults had lived with an alcohol
    abuser during childhood.41 Contact sexual abuse was
    reported by 22% of respondents (28% of women and
    16% of men) in our study. A national telephone survey
    of adults in 1990 using similar criteria for sexual abuse
    estimated that 27% of women and 16% of men had
    been sexually abused.12

    There are several reasons to believe that our esti-
    mates of the long-term relationship between adverse
    childhood experiences and adult health are conserva-
    tive. Longitudinal follow-up of adults whose childhood
    abuse was well documented has shown that their retro-
    spective reports of childhood abuse are likely to under-

    estimate actual occurrence.47,48 Underestimates of
    childhood exposures would result in downwardly bi-
    ased estimates of the relationships between childhood
    exposures and adult health risk behaviors and dis-
    eases. Another potential source of underestimation
    of the strength of these relationships is the lower
    number of childhood exposures reported by older
    persons in our study. This may be an artifact caused
    by premature mortality in persons with multiple
    adverse childhood exposures; the clustering of mul-
    tiple risk factors among persons with multiple child-
    hood exposures is consistent with this hypothesis.
    Thus, the true relationships between adverse child-
    hood exposures and adult health risk behaviors,
    health status, and diseases may be even stronger than
    those we report.

    An essential question posed by our observations is,
    “Exactly how are adverse childhood experiences linked
    to health risk behaviors and adult diseases?” The link-

    Table 4. Number of categories of adverse childhood exposure and the adjusted odds of risk factors including current
    smoking, severe obesity, physical inactivity, depressed mood, and suicide attempt

    Health problem

    Number
    of
    categories

    Sample
    size
    (N)a

    Prevalence
    (%)b

    Adjusted
    odds
    ratioc

    95%
    confidence
    interval

    Current smokerd 0 3,836 6.8 1.0 Referent
    1 2,005 7.9 1.1 ( 0.9–1.4)
    2 1,046 10.3 1.5 ( 1.1–1.8)
    3 587 13.9 2.0 ( 1.5–2.6)

    4 or more 544 16.5 2.2 ( 1.7–2.9)
    Total 8,018 8.6 — —

    Severe obesityd

    (BMI $ 35)
    0 3,850 5.4 1.0 Referent
    1 2,004 7.0 1.1 ( 0.9–1.4)
    2 1,041 9.5 1.4 ( 1.1–1.9)
    3 590 10.3 1.4 ( 1.0–1.9)

    4 or more 543 12.0 1.6 ( 1.2–2.1)
    Total 8,028 7.1 — —

    No leisure-time
    physical activity

    0 3,634 18.4 1.0 Referent
    1 1,917 22.8 1.2 ( 1.1–1.4)
    2 1,006 22.0 1.2 ( 1.0–1.4)
    3 559 26.6 1.4 ( 1.1–1.7)

    4 or more 523 26.6 1.3 ( 1.1–1.6)
    Total 7,639 21.0 — —

    Two or more weeks of
    depressed mood in
    the past year

    0 3,799 14.2 1.0 Referent

    1 1,984 21.4 1.5 ( 1.3–1.7)
    2 1,036 31.5 2.4 ( 2.0–2.8)
    3 584 36.2 2.6 ( 2.1–3.2)

    4 or more 542 50.7 4.6 ( 3.8–5.6)
    Total 7,945 22.0 — —

    Ever attempted suicide 0 3,852 1.2 1.0 Referent
    1 1,997 2.4 1.8 ( 1.2–2.6)
    2 1,048 4.3 3.0 ( 2.0–4.6)
    3 587 9.5 6.6 ( 4.5–9.8)

    4 or more 544 18.3 12.2 (8.5–17.5)
    Total 8,028 3.5 — —

    aSample sizes will vary due to incomplete or missing information about health problems.
    bPrevalence estimates are adjusted for age.
    cOdds ratios adjusted for age, gender, race, and educational attainment.
    dIndicates information recorded in the patient’s chart before the study questionnaire was mailed.

    252 American Journal of Preventive Medicine, Volume 14, Number 4

    ing mechanisms appear to center on behaviors such as
    smoking, alcohol or drug abuse, overeating, or sexual
    behaviors that may be consciously or unconsciously
    used because they have immediate pharmacological or
    psychological benefit as coping devices in the face of
    the stress of abuse, domestic violence, or other forms of

    family and household dysfunction. High levels of expo-
    sure to adverse childhood experiences would expect-
    edly produce anxiety, anger, and depression in chil-
    dren. To the degree that behaviors such as smoking,
    alcohol, or drug use are found to be effective as coping
    devices, they would tend to be used chronically. For

    Table 5. Number of categories of adverse childhood exposure and the prevalence and risk (adjusted odds ratio) of health
    risk factors including alcohol or drug abuse, high lifetime number of sexual partners, or history of sexually
    transmitted disease

    Health problem

    Number
    of
    categories

    Sample
    size
    (N)a

    Prevalence
    (%)b

    Adjusted
    odds
    ratioc

    95%
    confidence
    interval

    Considers self an
    alcoholic

    0 3,841 2.9 1.0 Referent
    1 1,993 5.7 2.0 (1.6–2.7)
    2 1,042 10.3 4.0 (3.0–5.3)
    3 586 11.3 4.9 (3.5–6.8)
    4 or more 540 16.1 7.4 (5.4–10.2)

    Total 8,002 5.9 — —
    Ever used illicit drugs 0 3,856 6.4 1.0 Referent

    1 1,998 11.4 1.7 (1.4–2.0)
    2 1,045 19.2 2.9 (2.4–3.6)
    3 589 21.5 3.6 (2.8–4.6)
    4 or more 541 28.4 4.7 (3.7–6.0)

    Total 8,029 11.6 — —
    Ever injected drugs 0 3,855 0.3 1.0 Referent

    1 1,996 0.5 1.3 (0.6–3.1)
    2 1,044 1.4 3.8 (1.8–8.2)
    3 587 2.3 7.1 (3.3–15.5)
    4 or more 540 3.4 10.3 (4.9–21.4)

    Total 8,022 0.8 — —
    Had 50 or more
    intercourse partners

    0 3,400 3.0 1.0 Referent
    1 1,812 5.1 1.7 (1.3–2.3)
    2 926 6.1 2.3 (1.6–3.2)
    3 526 6.3 3.1 (2.0–4.7)
    4 or more 474 6.8 3.2 (2.1–5.1)

    Total 7,138 4.4 — —
    Ever had a sexually
    transmitted diseased

    0 3,848 5.6 1.0 Referent
    1 2,001 8.6 1.4 (1.1–1.7)
    2 1,044 10.4 1.5 (1.2–1.9)
    3 588 13.1 1.9 (1.4–2.5)
    4 or more 542 16.7 2.5 (1.9–3.2)

    Total 8023 8.2 — —
    aSample sizes will vary due to incomplete or missing information about health problems.
    bPrevalence estimates are adjusted for age.
    cOdds ratios adjusted for age, gender, race, and educational attainment.
    dIndicates information recorded in the patient’s chart before the study questionnaire was mailed.

    Table 6. Relationship between number of categories of childhood exposure and number of risk factors for the leading
    causes of deatha

    Number of categories
    Sample
    size

    % with number of risk factors

    0 1 2 3 4

    0 3,861 56 29 10 4 1
    1 2,009 42 33 16 6 2
    2 1,051 31 33 20 10 4
    3 590 24 33 20 13 7

    $4 545 14 26 28 17 7
    Total 8,056 44 31 15 7 3

    aRisk factors include: smoking, severe obesity, physical inactivity, depressed mood, suicide attempt, alcoholism, any drug use, injected drug use,
    $50 lifetime sexual partners, and history of a sexually transmitted disease.

    Am J Prev Med 1998;14(4) 253

    example, nicotine is recognized as having beneficial
    psychoactive effects in terms of regulating affect49 and
    persons who are depressed are more likely to
    smoke.50,51 Thus, persons exposed to adverse child-
    hood experiences may benefit from using drugs such as
    nicotine to regulate their mood.49,52

    Consideration of the positive neuroregulatory effects
    of health-risk behaviors such as smoking may provide
    biobehavioral explanations53 for the link between ad-
    verse childhood experiences and health risk behaviors
    and diseases in adults. In fact, we found that exposure
    to higher numbers of categories of adverse childhood
    experiences increased the likelihood of smoking by the
    age of 14, chronic smoking as adults, and the presence
    of smoking-related diseases. Thus, smoking, which is
    medically and socially viewed as a “problem” may, from
    the perspective of the user, represent an effective
    immediate solution that leads to chronic use. Decades
    later, when this “solution” manifests as emphysema,
    cardiovascular disease, or malignancy, time and the

    tendency to ignore psychological issues in the manage-
    ment of organic disease make improbable any full
    understanding of the original causes of adult disease
    (Figure 2). Thus, incomplete understanding of the
    possible benefits of health risk behaviors leads them to
    be viewed as irrational and having solely negative
    consequences.

    Because adverse childhood experiences are common
    and they have strong long-term associations with adult
    health risk behaviors, health status, and diseases, in-
    creased attention to primary, secondary, and tertiary
    prevention strategies is needed. These strategies in-
    clude prevention of the occurrence of adverse child-
    hood experiences, preventing the adoption of health
    risk behaviors as responses to adverse experiences
    during childhood and adolescence, and, finally, help-
    ing change the health risk behaviors and ameliorating
    the disease burden among adults whose health prob-
    lems may represent a long-term consequence of ad-
    verse childhood experiences.

    Table 7. Number of categories of adverse childhood exposure and the prevalence and risk (adjusted odds ratio) of heart
    attack, cancer, stroke, COPD, and diabetes

    Disease conditiond

    Number
    of
    categories

    Sample
    size
    (N)a

    Prevalence
    (%)b

    Adjusted
    odds
    ratioc

    95%
    confidence
    interval

    Ischemic heart disease 0 3,859 3.7 1.0 Referent
    1 2,009 3.5 0.9 (0.7–1.3)
    2 1,050 3.4 0.9 (0.6–1.4)
    3 590 4.6 1.4 (0.8–2.4)
    4 or more 545 5.6 2.2 (1.3–3.7)

    Total 8,022 3.8 — —
    Any cancer 0 3,842 1.9 1.0 Referent

    1 1,995 1.9 1.2 (1.0–1.5)
    2 1,043 1.9 1.2 (1.0–1.5)
    3 588 1.9 1.0 (0.7–1.5)
    4 or more 543 1.9 1.9 (1.3–2.7)

    Total 8,011 1.9 — —
    Stroke 0 3,832 2.6 1.0 Referent

    1 1,993 2.4 0.9 (0.7–1.3)
    2 1,042 2.0 0.7 (0.4–1.3)
    3 588 2.9 1.3 (0.7–2.4)
    4 or more 543 4.1 2.4 (1.3–4.3)

    Total 7,998 2.6 — —
    Chronic bronchitis or
    emphysema

    0 3,758 2.8 1.0 Referent
    1 1,939 4.4 1.6 (1.2–2.1)
    2 1,009 4.4 1.6 (1.1–2.3)
    3 565 5.7 2.2 (1.4–3.3)
    4 or more 512 8.7 3.9 (2.6–5.8)

    Total 7,783 4.0 — —
    Diabetes 0 3,850 4.3 1.0 Referent

    1 2,002 4.1 1.0 (0.7–1.3)
    2 1,046 3.9 0.9 (0.6–1.3)
    3 587 5.0 1.2 (0.8–1.9)
    4 or more 542 5.8 1.6 (1.0–2.5)

    Total 8,027 4.3 — —
    aSample sizes will vary due to incomplete or missing information about health problems.
    bPrevalence estimates are adjusted for age.
    cOdds ratios adjusted for age, gender, race, and educational attainment.
    dIndicates information recorded in the patient’s chart before the study questionnaire was mailed.

    254 American Journal of Preventive Medicine, Volume 14, Number 4

    Primary prevention of adverse childhood experi-
    ences has proven difficult54,55 and will ultimately re-
    quire societal changes that improve the quality of family
    and household environments during childhood. Recent
    research on the long-term benefit of early home visitation
    on reducing the prevalence of adverse childhood experi-
    ences is promising.56 In fact, preliminary data from the
    ACE Study provided the impetus for the Kaiser Health
    Plan to provide funding to participate at 4 locations
    (including San Diego County, California) in the Com-
    monwealth Fund’s “Healthy Steps” program. This pro-
    gram extends the traditional practice of pediatrics by
    adding one or more specialists in the developmental and
    psychosocial dimensions of both childhood and parent-
    hood. Through a series of office visits, home visits, and a
    telephone advice line for parents, these specialists develop
    close relationships between children and their families
    from birth to 3 years of age. This approach is consistent
    with the recommendation of the U.S. Advisory Board on
    Child Abuse and Neglect that a universal home visitation
    program for new parents be developed57,58 and provides
    an example of a family-based primary prevention effort
    that is being explored in a managed care setting. If these
    types of approaches can be replicated and implemented
    on a large scale, the long-term benefits may include,
    somewhat unexpectedly, substantial improvements in
    overall adult health.

    Secondary prevention of the effects of adverse child-
    hood experiences will first require increased recogni-
    tion of their occurrence and second, an effective un-

    derstanding of the behavioral coping devices that
    commonly are adopted to reduce the emotional impact
    of these experiences. The improbability of giving up an
    immediate “solution” in return for a nebulous long-
    term health benefit has thwarted many well-intended
    preventive efforts. Although articles in the general
    medical literature are alerting the medical community
    to the fact that childhood abuse is common,59 adoles-
    cent health care is often inadequate in terms of psycho-
    social assessment and anticipatory guidance.60 Clearly,
    comprehensive strategies are needed to identify and
    intervene with children and families who are at risk for
    these adverse experiences and their related out-
    comes.61 Such strategies should include increased com-
    munication between and among those involved in
    family practice, internal medicine, nursing, social work,
    pediatrics, emergency medicine, and preventive medi-
    cine and public health. Improved understanding is also
    needed of the effects of childhood exposure to domes-
    tic violence.19,62 Additionally, increased physician train-
    ing63 is needed to recognize and coordinate the man-
    agement of all persons affected by child abuse,
    domestic violence, and other forms of family adversity
    such as alcohol abuse or mental illness.

    In the meantime, tertiary care of adults whose health
    problems are related to experiences such as childhood
    abuse5 will continue to be a difficult challenge. The
    relationship between childhood experiences and adult
    health status is likely to be overlooked in medical
    practice because the time delay between exposure

    Table 8. Number of categories of adverse childhood exposure and the prevalence and risk (adjusted odds ratio) of skeletal
    fracture, hepatitis or jaundice, and poor self-rated health

    Disease condition

    Number
    of
    categories

    Sample
    size
    (N)a

    Prevalence
    (%)b

    Adjusted
    odds
    ratioc

    95%
    confidence
    interval

    Ever had a skeletal
    fracture

    0 3,843 3.6 1.0 Referent
    1 1,998 4.0 1.1 (1.0–1.2)
    2 1,048 4.5 1.4 (1.2–1.6)
    3 587 4.0 1.2 (1.0–1.4)
    4 or more 544 4.8 1.6 (1.3–2.0)

    Total 8,020 3.9 — —
    Ever had hepatitis or
    jaundice

    0 3,846 5.3 1.0 Referent
    1 2,006 5.5 1.1 (0.9–1.4)
    2 1,045 7.7 1.8 (1.4–2.3)
    3 590 10.2 1.6 (1.2–2.3)
    4 or more 543 10.7 2.4 (1.8–3.3)

    Total 8,030 6.5 — —
    Fair or poor self-rated
    health

    0 3,762 16.3 1.0 Referent
    1 1,957 17.8 1.2 (1.0–1.4)
    2 1,029 19.9 1.4 (1.2–1.7)
    3 584 20.3 1.4 (1.1–1.7)
    4 or more 527 28.7 2.2 (1.8–2.7)

    Total 7,859 18.2 — —
    aSample sizes will vary due to incomplete or missing information about health problems.
    bPrevalence estimates are adjusted for age and gender.
    cOdds ratios adjusted for age, gender, race, and educational attainment.
    dIndicates information recorded in the patient’s chart before the study questionnaire was mailed.

    Am J Prev Med 1998;14(4) 255

    during childhood and recognition of health problems
    in adult medical practice is lengthy. Moreover, these
    childhood exposures include emotionally sensitive top-
    ics such as family alcoholism29,30 and sexual abuse.64

    Many physicians may fear that discussions of sexual
    violence and other sensitive issues are too personal
    even for the doctor-patient relationship.65 For example,
    the American Medical Association recommends screen-
    ing of women for exposure to violence at every en-
    trance to the health system;66 however, such screening
    appears to be rare.67 By contrast, women who are asked
    about exposure to sexual violence say they consider
    such questions to be welcome and germane to routine
    medical care,68 which suggests that physicians’ fears
    about patient reactions are largely unfounded.

    Clearly, further research and training are needed to
    help medical and public health practitioners under-
    stand how social, emotional, and medical problems are
    linked throughout the lifespan (Figure 2). Such re-
    search and training would provide physicians with the
    confidence and skills to inquire and respond to patients
    who acknowledge these types of childhood exposures.
    Increased awareness of the frequency and long-term
    consequences of adverse childhood experiences may
    also lead to improvements in health promotion and
    disease prevention programs. The magnitude of the
    difficulty of introducing the requisite changes into

    medical and public health research, education, and
    practice can be offset only by the magnitude of the
    implications that these changes have for improving the
    health of the nation.

    We thank Naomi Howard for her dedication to the ACE Study.
    This research is supported by the Centers for Disease Control
    and Prevention via cooperative agreement TS-44-10/12 with the
    Association of Teachers of Preventive Medicine.

    References
    1. Springs F, Friedrich WN. Health risk behaviors and

    medical sequelae of childhood sexual abuse. Mayo Clin
    Proc 1992;67:527–32.

    2. Felitti VJ. Long-term medical consequences of incest,
    rape, and molestation. South Med J 1991;84:328–31.

    3. Felitti VJ. Childhood sexual abuse, depression and family
    dysfunction in adult obese patients: a case control study.
    South Med J 1993;86:732–6.

    4. Gould DA, Stevens NG, Ward NG, Carlin AS, Sowell HE,
    Gustafson B. Self-reported childhood abuse in an adult
    population in a primary care setting. Arch Fam Med
    1994;3:252–6.

    5. McCauley J, Kern DE, Kolodner K, Schroeder AF, et al.
    Clinical characteristics of women with a history of child-
    hood abuse. JAMA 1997;277:1362–8.

    Figure 2. Potential influences throughout the lifespan of adverse childhood experiences.

    256 American Journal of Preventive Medicine, Volume 14, Number 4

    6. Mortality patterns: United States, 1993. Morb Mortal
    Wkly Rep 1996;45:161–4.

    7. McGinnis JM, Foege WH. Actual causes of death in the
    United States. JAMA 1993;270:2207–12.

    8. Landis J. Experiences of 500 children with adult sexual
    deviation. Psychiatr Q 1956;30(Suppl):91–109.

    9. Straus MA, Gelles RJ. Societal change and change in
    family violence from 1975 to 1985 as revealed by two
    national surveys. J Marriage Family 1986;48:465–79.

    10. Wyatt GE, Peters SD. Methodological considerations in
    research on the prevalence of child sexual abuse. Child
    Abuse Negl 1986;10:241–51.

    11. Berger AM, Knutson JF, Mehm JG, Perkins KA. The
    self-report of punitive childhood experiences of young
    adults and adolescents. Child Abuse Negl 1988;12:251–
    62.

    12. Finkelhor D, Hotaling G, Lewis IA, Smith C. Sexual abuse
    in a national survey of adult men and women: prevalence,
    characteristics, and risk factors. Child Abuse Negl 1990;
    14:19–28.

    13. Egelend B, Sroufe LA, Erickson M. The developmental
    consequence of different patterns of maltreatment. Child
    Abuse Negl 1983;7:459–69.

    14. Finkelhor D, Browne A. The traumatic impact of child
    sexual abuse Am J Orthopsychiatry. 1985;55:530–41.

    15. Beitchman JH, Zucker KJ, Hood JE, DaCosta GA, Akman
    D, Cassavia E. A review of the long-term effects of sexual
    abuse. Child Abuse Negl 1992;16:101–18.

    16. Hibbard RA, Ingersoll GM, Orr DP. Behavioral risk,
    emotional risk, and child abuse among adolescents in a
    nonclinical setting. Pediatrics 1990;86:896–901.

    17. Nagy S, Adcock AG, Nagy MC. A comparison of risky
    health behaviors of sexually active, sexually abused, and
    abstaining adolescents. Pediatrics 1994;93:570–5.

    18. Cunningham RM, Stiffman AR, Dore P. The association
    of physical and sexual abuse with HIV risk behaviors in
    adolescence and young adulthood: implications for pub-
    lic health. Child Abuse Negl 1994;18:233–45.

    19. Council on Scientific Affairs. Adolescents as victims of
    family violence. JAMA 1993;270:1850–6.

    20. Nelson DE, Higginson GK, Grant-Worley JA. Physical
    abuse among high school students. Prevalence and cor-
    relation with other health behaviors. Arch Pediatr Ado-
    lesc Med 1995;149:1254–8.

    21. Mullen PE, Roman-Clarkson SE, Walton VA, Herbison
    GP. Impact of sexual and physical abuse on women’s
    mental health. Lancet 1988;1:841–5.

    22. Drossman DA, Leserman J, Nachman G, Li Z, et al. Sexual
    and physical abuse in women with functional or organic
    gastrointestinal disorders. Ann Intern Med 1990;113:
    828–33.

    23. Harrop-Griffiths J, Katon W, Walker E, Holm L, Russo J,
    Hickok L. The association between chronic pelvic pain,
    psychiatric diagnoses, and childhood sexual abuse. Ob-
    stet Gynecol 1988;71:589–94.

    24. Briere J, Runtz M. Multivariate correlates of childhood
    psychological and physical maltreatment among univer-
    sity women. Child Abuse Negl 1988;12:331–41.

    25. Briere J, Runtz M. Differential adult symptomatology
    associated with three types of child abuse histories. Child
    Abuse Negl 1990;14:357–64.

    26. Claussen AH, Crittenden PM. Physical and psychological
    maltreatment: relations among types of maltreatment.
    Child Abuse Negl 1991;15:5–18.

    27. Moeller TP, Bachman GA, Moeller JR. The combined
    effects of physical, sexual, and emotional abuse during
    childhood: long-term health consequences for women.
    Child Abuse Negl 1993;17:623–40.

    28. Bryant SL, Range LM. Suicidality in college women who
    were sexually and physically punished by parents. Vio-
    lence Vict 1995;10:195–201.

    29. Zeitlen H. Children with alcohol misusing parents. Br
    Med Bull 1994;50:139–51.

    30. Dore MM, Doris JM, Wright P. Identifying substance
    abuse in maltreating families: a child welfare challenge.
    Child Abuse Negl 1995;19:531–43.

    31. Ethier LS, Lacharite C, Couture G. Childhood adversity,
    parental stress, and depression of negligent mothers.
    Child Abuse Negl 1995;19:619–32.

    32. Spaccarelli S, Coatsworth JD, Bowden BS. Exposure to
    family violence among incarcerated boys; its association
    with violent offending and potential mediating variables.
    Violence Vict 1995;10:163–82.

    33. McCloskey LA, Figueredo AJ, Koss MP. The effects of
    systemic family violence on children’s mental health.
    Child Dev 1995;66:1239–61.

    34. Brent DA, Perper JA, Moritz G, Schweers J, Balach L,
    Roth C. Familial risk factors for adolescent suicide: a
    case-control study. Acta Psychiatr Scand 1994;89:52–8.

    35. Shaw DS, Vondra JI, Hommerding KD, Keenan K, Dunn
    M. Chronic family adversity and early child behavior
    problems: a longitudinal study of low income families.
    J Child Psychol Psychiatry 1994;35:1109–22.

    36. U.S. Department of Health and Human Services. Physical
    activity and health: A report of the Surgeon General.
    Atlanta, Georgia. U.S. Department of Health and Human
    Services, Centers for Disease Control and Prevention,
    National Center for Chronic Disease Prevention and
    Health Promotion; 1996.

    37. Rivara FP, Mueller BA, Somes G, Mendoza CT, Rushforth
    NB, Kellerman AL. Alcohol and illicit drug abuse and the
    risk of violent death in the home. JAMA 1997;278:569–
    75.

    38. Dillman DA. Mail and telephone surveys: the total design
    method. New York: John Wiley & Sons; 1978.

    39. Straus M, Gelles RJ. Physical violence in American fami-
    lies: risk factors and adaptations to violence in 8,145
    families. New Brunswick: Transaction Press; 1990.

    40. Wyatt GE. The sexual abuse of Afro-American and White-
    American women in childhood. Child Abuse Negl 1985;
    9:507–19.

    41. National Center for Health Statistics. Exposure to alco-
    holism in the family: United States, 1988. Advance Data,
    No. 205. U.S. Department of Health and Human Services,
    Washington, DC; September 30, 1991.

    42. Siegel PZ, Frazier EL, Mariolis P, et al. Behaviorial risk
    factor surveillance, 1991; Monitoring progress toward the
    Nation’s Year 2000 Health Objectives. Morb Mortal Wkly
    Rep 1992;42(SS-4).1–15.

    43. Crespo CJ, Keteyian SJ, Heath GW, Sempos CT. Leisure-
    time physical activity among US adults: Results from the

    Am J Prev Med 1998;14(4) 257

    Third National Health and Nutrition Examination Sur-
    vey. Arch Intern Med 1996;156:93–8.

    44. Robins LN, Helzer JE, Groughan J, Ratliff K. National
    Institute of Mental Health Diagnostic Interview Schedule:
    its history, characteristics, and validity. Arch Gen Psychi-
    atry 1981;38:381–9.

    45. Idler E, Angel RJ. Self-rated health and mortality in the
    NHANES I Epidemiologic Follow-up Study. Am J Pub
    Health 1990;80:446–52.

    46. SAS Procedures Guide. SAS Institute Inc. Version 6, 3rd
    edition, Cary, NC: SAS Institute; 1990.

    47. Femina DD, Yeager CA, Lewis DO. Child abuse: adoles-
    cent records vs. adult recall. Child Abuse Negl 1990;14:
    227–31.

    48. Williams LM. Recovered memories of abuse in women
    with documented child sexual victimization histories.
    J Traumatic Stress 1995;8:649–73.

    49. Carmody TP. Affect regulation, nicotine addiction, and
    smoking cessation. J Psychoactive Drugs 1989;21:331–42.

    50. Anda RF, Williamson DF, Escobedo LG, Mast EE, Giovino
    GA, Remington PL. Depression and the dynamics of
    smoking. A national perspective. JAMA 1990;264:1541–5.

    51. Glassman AH, Helzer JE, Covey LS, Cottler LB, Stetner F,
    Tipp JE, Johnson J. Smoking, smoking cessation, and
    major depression. JAMA 1990;264:1546–9.

    52. Hughes JR. Clonidine, depression, and smoking cessa-
    tion. JAMA 1988;259:2901–2.

    53. Pomerlau OF, Pomerlau CS. Neuroregulators and the
    reinforcement of smoking: towards a biobehavioral expla-
    nation. Neurosci Biobehav Rev 1984;8:503–13.

    54. Hardy JB, Street R. Family support and parenting educa-
    tion in the home: an effective extension of clinic-based
    preventive health care services for poor children. J Pedi-
    atr 1989;115:927–31.

    55. Olds DL, Henderson CR, Chamberlin R, Tatelbaum R.
    Preventing child abuse and neglect: a randomized trial of
    nurse home visitation. Pediatrics 1986;78:65–78.

    56. Olds DL, Eckenrode J, Henderson CR, Kitzman H, et al.
    Long-term effects of home visitation on maternal life
    course and child abuse and neglect: Fifteen-year fol-
    low-up of a randomized trial. JAMA 1997;278:637–43.

    57. U.S. Advisory Board on Child Abuse and Neglect. Child
    abuse and neglect: critical first steps in response to a
    national emergency. Washington, DC: U.S. Government
    Printing Office; August 1990; publication no. 017-092-
    00104-5.

    58. U.S. Advisory Board on Child Abuse and Neglect. Creat-
    ing caring communities: blueprint for an effective federal
    policy on child abuse and neglect. Washington, DC: U.S.
    Government Printing Office; September 1991.

    59. MacMillan HL, Fleming JE, Trocme N, Boyle MH, et al.
    Prevalence of child physical and sexual abuse in the
    community. Results from the Ontario Health Supple-
    ment. JAMA 1997;278:131–5.

    60. Rixey S. Family violence and the adolescent. Maryland
    Med J 1994;43:351–3.

    61. Chamberlin RW. Preventing low birth weight, child
    abuse, and school failure: the need for comprehensive,
    community-wide approaches. Pediatr Rev 1992;13:64–71.

    62. Kashani JH, Daniel AE, Dandoy AC, Holcomb WR. Family
    violence: impact on children. J Am Acad Child Adolesc
    Psychiatry 1992;31:181–9.

    63. Dubowitz H. Child abuse programs and pediatric resi-
    dency training. Pediatrics 1988;82:477–80.

    64. Tabachnick J, Henry F, Denny L. Perceptions of child
    sexual abuse as a public health problem. Vermont, Sep-
    tember 1995. Morb Mortal Wkly Rep 1997;46:801–3.

    65. Sugg NK, Inui T. Primary care physicians’ response to
    domestic violence. Opening Pandora’s box. JAMA 1992;
    267:3157–60.

    66. Council on Scientific Affairs. American Medical Associa-
    tion Diagnostic and Treatment Guidelines on Domestic
    Violence. Arch Fam Med 1992;1:38–47.

    67. Hamberger LK, Saunders DG, Hovey M. Prevalence of
    domestic violence in community practice and rate of
    physician inquiry. Fam Med 1992;24:283–7.

    68. Friedman LS, Samet JH, Roberts MS, Hans P. Inquiry
    about victimization experiences. A survey of patient pref-
    erences and physician practices. Arch Int Med 1992;152:
    1186–90.

    258 American Journal of Preventive Medicine, Volume 14, Number 4

    Posttraumatic Stress in Youth
    Experiencing Illnesses and Injuries:
    An Exploratory Meta-Analysi

    s

    Shoshana Y

    .

    Kahana, Norah C. Feeny,
    Eric A. Youngstrom, and Dennis Drotar

    tumors, and non-Hodgkin’s lymphoma (Woodruff
    et al., 2004). More than 11,000 children are diagnosed
    with new cancers each year in the United States,
    and there are currently an estimated 250,000 pedi-
    atric cancer survivors. Furthermore, from 1988 to
    present, between 7% and 9.5% of all organ trans-
    plants conducted annually in the United States wer

    e

    performed with pediatric populations (Transpla

    n

    t

    DataSource, 2001).

    Despite the high prevalence rates of both injuri

    es

    and illnesses among youth, little is understood about
    the psychological sequelae that are related to both
    conditions. Recently, researchers have posited that
    posttraumatic stress disorder (PTSD) may be a use-
    ful construct to conceptualize some of the psycho-
    logical distress and loss of functioning that is
    present in a significant subset of individuals following
    illnesses or injuries. Consistent with the Diagnostic
    and Statistical Manual, Fourth Edition (DSM-IV;
    American Psychiatric Association, 2001), a serious
    or life-threatening illness or injury usually contai

    ns

    several traumatic elements, including a “real or

    The incidence of injuries and illnesses (e.g.,
    cancer and organ transplantation) among
    youth is high. Injuries have been the largest

    cause of morbidity and mortality among children in
    the United States for many years (Guyer et al.,
    1999). Close to 20 million children suffer uninten-
    tional injuries annually, and motor vehicle acciden

    ts

    (MVAs) and fires are among the leading causes of
    unintentional injuries in youth through the ages of 19
    (Public Health Policy Advisory Board, 1999). With
    respect to medical illnesses, recent epidemiological
    data suggest that the incidence of certain childhood
    cancers has steadily increased since 1990, including
    acute lymphoblastic leukemia, central nervous system

    To date there is no quantitative review of predictors of
    posttraumatic stress disorder (PTSD) symptoms in youth
    experiencing illnesses or injuries. This article presents

    a

    meta-analysis of variables associated with the develop-
    ment of PTSD among those youth. Twenty-six studies
    were included: 18 involving children experiencing
    injuries and 8 with pediatric illnesses. Among injured
    youth, socioeconomic status and social impairment were
    small to moderate correlates of PTSD, whereas depres-
    sive and anxious symptoms, dissociation, acute stress dis-
    order, and the appraisal of trauma severity and life threat
    displayed large effect sizes with PTSD severity. Among ill

    youth, social support and the appraisal of illness severity
    and life threat emerged as small to moderate predictors
    of posttraumatic symptoms. The current findings are
    exploratory in nature, as a primary limitation of the cur-
    rent study includes the limited number of independe

    nt

    studies that have evaluated these predictors. Current
    findings further our understanding of PTSD through
    exploration of possible indicators of at-risk youth who
    have experienced illness and injury.

    Keywords: PTSD; youth; injuries; medical illness;
    predictors

    From Case Western Reserve University, Cleveland, Ohio (SYK,
    NCF, EAY, DD); Rainbow Babies & Children’s Hospital,
    Division of Behavioral Pediatrics & Psychology, Cleveland,
    Ohio (DD).

    Address correspondence to: Shoshana Y. Kahana, Case Western
    Reserve University, Department of Psychology, 11220 Bellflower
    Rd., Cleveland, OH 44106; e-mail: shoshana.kahana@case.edu.

    Traumatology
    Volume 12 Number 2

    June 2006 148-161
    © 2006 Sage Publications

    10.1177/1534765606294562
    http://tmt.sagepub.com

    hosted at
    http://online.sagepub.com

    148

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed

    b

    y
    th

    e

    A

    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    t

    io
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r

    t
    he

    p
    er

    so
    na

    l

    u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r

    a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    threatened death or a threat to the physical integrity
    of self and others.” In addition to the injury or illness
    itself, life-threatening conditions and their treat-
    ments typically involve a series of potential traumas,
    such as the life threat inherent in some of the “high-
    tech” procedures involved in treatment, the repeated
    intrusions of the procedures, and the experienced
    pain (Stuber, Nader, Houskamp, & Pynoos, 1996).
    Moreover, both injuries and illnesses frequently
    evoke intense feelings of fear, uncertainty, and help-
    lessness about future outcomes and functioning.

    Although many individuals exposed to trauma do
    not develop PTSD, a substantial minority do. Indeed,
    researchers have reported significant rates of PTS

    D

    as well as posttraumatic stress symptoms (PTSSs) in
    children experiencing various illness-related trau-
    mas, such as cancer (Kazak et al., 2004) and liver
    transplants (Shemesh et al., 2000), or injuries, such
    as MVAs (Bryant, Mayou, Wiggs, Ehlers, & Stores,
    2004), traumatic brain injury (TBI; Levi, Drotar,
    Yeates, & Taylor, 1999), and burns (Saxe, Stoddard,
    & Sheridan, 1998). Pelcovitz et al. (1998) reported
    that 35% of adolescents with cancer were diagnosed
    with PTSD, whereas 34.5% of youth involved in
    MVAs met criteria for PTSD (Stallard, Velleman, &
    Baldwin, 1998).

    It is important to note that the clinical signifi-
    cance of subthreshold or partial PTSD (i.e., exhibit-
    ing some but not all PTSD symptoms) also has
    begun to be addressed in the literature. For example,
    research has noted that among youth who had expe-
    rienced acute physical injury, many suffered with
    subthreshold levels of PTSD, which was associated
    with internalizing symptoms, such as depression and
    anxiety (Aaron, Zaglul, & Emery, 1999). In addition,
    Marshall et al. (2001) reported that the presence of
    subthreshold PTSSs significantly increased the ris

    k

    for suicidal ideation, comorbidity, and overall greater
    impairment. Thus, the clinical relevance of sub-
    threshold PTSD is an important construct to con-
    sider among individuals exposed to various traumas.

    Because of the relatively high incidence of PTSD
    among youth experiencing injuries and illnesses, it is
    crucial to identify children at high risk for the devel-
    opment of PTSD after these traumas. If left
    untreated, PTSD may affect many domains, including
    social functioning (Berman, Kurtines, Silverman, &
    Serafini, 1996; Stallard, Velleman, Langsford, &
    Baldwin, 2001), physical recovery from illnesses or
    injuries (Stallard et al., 2001), and academic function-
    ing (Reinherz, Giaconia, Lefkowitz, Pakiz, & Frost,

    1993). The identification of potential predictors of
    PTSD among youth experiencing illness- or injury-
    related traumas may also facilitate efforts to effec-
    tively address emergent psychiatric symptoms.

    Currently, we know a good deal about predictors
    for the development of PTSD in adults, but we know
    substantially less about predictors for youth exposed to
    illness- or injury-related traumas. Some early research
    (La Greca, Silverman, Vernberg, & Prinstein, 1996;
    Vernberg, Silverman, La Greca, & Prinstein, 1996

    )

    has been influential in introducing a classification
    model for the predictors of PTSD in traumatized
    youth, which includes the child’s characteristics
    (i.e., gender, age), characteristics of the stressor
    (i.e., amount of time exposed to stressor, life threat
    involved), characteristics of the environment (i.e.,
    access to social support, family psychiatric history),
    and cognitive processing (i.e., coping) of the trau-
    matic event. Although some qualitative work has
    described various predictors of PTSD among youth
    with illnesses and injuries (e.g., Stuber, Shemesh, &
    Saxe, 2003), no research to date has derived quanti-
    tative estimates for these predictors. Similarly,
    meaningful descriptive data across various studies,
    such as the aggregation of the number and types of
    PTSSs, has not been reported for youth experienc-
    ing illnesses and injuries.

    Finally, although illnesses and injuries clearly share
    some significant commonalities (e.g., can involve life
    threat, affect family functioning) and have often been
    treated as the same construct, the literature has not
    addressed differences that may exist between these
    two traumas. In fact, it is likely that individuals who
    experience illness as compared to injury are exposed to
    different traumatic events. Furthermore, it is the expo-
    sure to these events that defines the traumatic experi-
    ence of illnesses and injuries. For example, among
    youth exposed to more acute traumas, typically involv-
    ing injuries, the aftermath may include additional
    traumatic stressors, such as aspects of the emergency
    medical setting and/or the deaths of other individua

    ls

    involved in the traumatic event. Conversely, for youth
    with illnesses, there are the uncertainties of second
    malignancies (cancer) or organ rejection (transplantees)
    as well as the painfulness of certain treatments (e.g.,
    chemotherapy). In short, combining injuries and ill-
    nesses into the same construct may obscure some of
    the subtle yet important nuances that are inherent
    to each.

    The aim of this study is twofold. The authors
    synthesize descriptive information along with PTSD

    Meta-Analysis of PTSD in Youth / Kahana et al. 149

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    prevalence rates and symptoms among youth who
    were exposed to illnesses or injuries. In addition, the
    authors conduct a meta-analysis to identify consis-
    tent variables associated with the development of
    PTSD among youth experiencing illnesses or injuries.
    To address what we perceive to be the gap in the lit-
    erature, the phenomenology of PTSD among youth
    experiencing illnesses and injuries will be investi-
    gated separately and, when possible, will be directly
    compared to each other.

    Method

    Literature Search

    The authors conducted comprehensive literature
    searches using various medical and psychological
    bibliographic databases, such as PsycINFO and
    MEDLINE, to find articles that reported the preva-
    lence rates as well as data on the predictors for PTSD
    (as defined by DSM-III, DSM-III-R, and DSM-IV) in
    youth between the ages of 6 and 19. Search terms
    such as trauma, posttraumatic stress disorder, post-
    traumatic stress symptoms, PTSD, PTSS, stress, child,
    adolescent, youth, medical, traffic accidents, motor
    vehicle accidents, injury, cancer, burns, transplant,
    predictor(s), prevalence, and epidemiology were uti-
    lized in the search for articles. In addition, the refer-
    ence sections of all of these identified articles were
    examined to glean additional articles. Finally, several
    researchers noted for their work in pediatric trau-
    matic stress were contacted to access data that were
    included in manuscripts in press.

    Criteria for Inclusion and Exclusion

    The studies included for the current review used
    quantitative methods to examine the prevalence
    rates of PTSD, as well as the potential predictors of
    PTSD, in youth experiencing illnesses or injuries. In
    accordance with the current classifications of
    numerous government, medical, and public policy
    organizations (e.g., National Center for

    Injury

    Prevention and Control), ill youth were defined as
    those experiencing relatively acute medical illnesses,
    such as cancer or liver transplants, whereas injury-
    related traumas were defined as those involving
    MVAs, burns, or brain injury. Injuries that were the
    result of violent physical interpersonal traumas,
    such as assaults and muggings, were not included in
    this study. A predictor was defined as any variable

    that contributed to variability in PTSD diagnostic
    status or symptom severity. The authors opted for
    the use of terms such as predictors and correlates, as
    compared to risk factors, owing to the developing
    and emergent state of the current medical trauma
    literature.

    Several additional inclusionary and exclusionary
    criteria were applied to the current study. Only
    English-language articles published in peer-reviewed
    journals (from 1980 onward) were included, as the
    diagnosis of PTSD as applied to children only
    emerged in the 1980s. The use of only published
    studies has received some recent support from
    research suggesting that a publication bias does not
    necessarily exist among certain medical and social
    science journals (Olson et al., 2002). Finally, this
    review included studies that assessed for a DSM
    diagnosis of PTSD or PTSD symptom severity.

    Articles were excluded on the following grounds:
    (a) they did not utilize a diagnostic instrument
    or measure that could assess all relevant PTSSs,
    (b) they were based on case studies, (c) they were
    based on a sample that was primarily composed of
    individuals older than 19 years of age, and (d) they
    did not include sufficient statistical data to compute
    meaningful analyses. As such, 26 empirical studies
    were included in this review—18 of which described
    injured youth, whereas 8 involved youth with various
    illnesses (see Table 1 for a description of studies).

    Methodological Considerations

    The studies included in this meta-analysis utilized a
    wide range of research designs and effect size esti-
    mates. As such, the current authors employed various
    strategies to ensure methodological accuracy. For
    example, if the same sample was used in several stud-
    ies, it was only included once in the meta-analysis,
    whereas multiple dependent variables based on the
    same sample were aggregated across studies. This
    was done to avoid a significant distortion of the
    standard error estimates that typically results when
    treating nonindependent studies as independent
    (Gleser & Olkin, 1994). In addition, when a study
    reported multiple effect size estimates for the same
    general construct, they were averaged to calculate
    an overall effect size.

    To compute prevalence rates of PTSD, the authors
    decided that scores falling in the moderate and
    severe ranges of continuous assessment measures
    would qualify as roughly meeting PTSD diagnostic

    150 Traumatology / Vol. 12, No. 2, June 2006

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Ta
    bl

    e
    1.

    D
    es

    cr
    ip

    ti
    on

    o
    f

    S

    tu

    di
    es

    I

    nc

    lu
    de

    d
    in

    t
    he

    M
    et

    a-
    A

    na
    ly

    si
    s

    S
    am

    pl
    e

    S
    iz

    e
    of

    Ty
    pe

    o
    f

    Tr
    au

    m
    at

    iz
    ed

    C

    om

    pa
    ri

    so
    n

    T
    im

    e
    C

    ur
    re

    nt
    o

    r
    D

    ia
    gn

    os
    ti

    c
    A

    ut
    ho

    r
    S

    am
    pl

    e
    Yo

    ut
    h

    G
    ro

    up
    s

    F
    ra

    m
    e

    L

    if

    et
    im

    e
    In

    fo
    rm

    an
    ts

    In
    st

    ru
    m

    en
    ts

    B
    ro

    w
    n,

    M
    ad

    an

    S
    w

    ai
    n,

    &
    L

    am
    be

    rt
    (

    20
    03

    )

    B
    ut

    le
    r,

    R

    iz

    zi
    ,

    &
    H

    an
    dw

    er
    ge

    r
    (1

    99
    6)

    K
    az

    ak
    e

    t

    al

    .
    (1

    99
    7)

    a

    K
    az

    ak
    e

    t
    al

    .
    (2

    00
    4)

    b

    Pe
    lc

    ov
    it

    z
    et

    a
    l.

    (1
    99

    8)

    S
    he

    m
    es

    h
    et

    a
    l.

    (2
    00

    0)

    S
    tu

    be
    r,

    N
    ad

    er
    ,

    H
    ou

    sk
    am

    p,
    &

    P
    yn

    oo
    s

    (1
    99

    6)
    c

    W
    al

    ke
    r,

    H
    ar

    ri
    s,

    B
    ak

    er
    ,

    K
    el

    ly
    ,

    &
    H

    ou
    gh

    to
    n

    (1
    99

    9)

    A
    ar

    on
    ,

    Za
    gl

    ul
    ,

    &
    E

    m
    er

    y
    (1

    99
    9)

    B
    ry

    an
    t,

    M
    ay

    ou
    ,

    W
    ig

    gs
    , E

    hl
    er

    s,
    &

    S
    to

    re
    s

    (2
    00

    4)
    d

    A
    do

    le
    sc

    en
    t

    su
    rv

    iv
    or

    s
    of

    ch
    i

    ld

    ho
    od

    c
    an

    ce
    r

    Pe
    di

    at
    ri

    c
    ca

    nc
    er

    su
    rv

    iv
    or

    s

    C
    hi

    ld
    ho

    od
    l

    eu
    ke

    m
    ia

    su
    rv

    iv
    or

    s

    A
    do

    le
    sc

    en
    t

    su
    rv

    iv
    or

    s
    of

    ch
    ild

    ho
    od

    c
    an

    ce
    r

    C
    an

    ce
    r

    L
    iv

    er
    t

    ra
    ns

    pl
    an

    ts

    Pe
    di

    at
    ri

    c
    ca

    nc
    er

    (
    bo

    ne
    m

    ar
    ro

    w
    t

    ra
    ns

    pl
    an

    ts
    ;

    B
    M

    T
    )

    L
    iv

    er
    t

    ra
    ns

    pl
    an

    ts

    P
    hy

    si
    ca

    l
    in

    ju
    ri

    es

    M
    VA

    52 72 13
    0

    15
    0

    23 19 30 18 40 81

    C
    an

    ce
    r

    su
    rv

    iv
    or

    s
    vs

    .
    he

    al
    th

    y
    co

    nt
    ro

    ls

    P
    T

    S
    D

    v
    s.

    n
    on

    -P

    T

    S
    D

    C
    an

    ce
    r

    su
    rv

    iv
    or

    s
    vs

    .
    he

    al
    th

    y
    co

    nt
    ro

    ls

    S
    ur

    vi
    vo

    rs
    r

    ec
    ei

    vi
    ng

    ps
    yc

    ho
    so

    ci
    al

    t
    re

    at
    m

    en
    t

    vs
    .

    su
    rv

    iv
    or

    w
    ai

    t-
    lis

    t
    co

    nt
    ro

    ls

    P
    hy

    si
    ca

    l
    in

    ju
    ry

    ;
    hi

    gh
    -r

    is
    k

    vs
    .

    lo
    w

    -r
    is

    k

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    L
    iv

    er
    t

    ra
    ns

    pl
    an

    te
    es

    v
    s.

    y
    ou

    th
    w

    it
    h

    ei
    th

    er
    c

    hr
    on

    ic
    a

    st
    hm

    a
    or

    t
    ho

    se
    u

    nd
    er

    go
    in

    g
    E

    N
    T

    op
    er

    at
    io

    ns

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    R R R R
    a

    nd
    P

    R R P R P P

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    U
    nc

    le
    ar

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    C
    ur

    re
    nt

    ?

    U
    nc

    le
    ar

    L
    if

    et
    im

    e
    an

    d
    C

    ur
    re

    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    Pa
    re

    nt

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t
    (o

    nl
    y

    ch
    ild

    f
    or

    P
    T

    S
    D

    )

    C
    hi

    ld

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t
    (o

    nl
    y

    ch
    ild

    f
    or

    P
    T

    S
    D

    )

    P
    T

    S
    D

    -R
    I

    P
    S

    S

    IE
    S

    /P
    T

    S
    D

    -R
    I/

    T
    S

    C

    C
    P

    T
    S

    D
    -R

    I/
    IE

    S
    -R

    /

    S
    C

    ID

    S
    C

    ID
    /D

    IS

    U
    C

    L
    A

    -P
    T

    S
    D

    -R
    I

    C
    P

    T
    S

    D
    -R

    I

    C
    P

    T
    S

    -R
    I

    P
    T

    S
    D

    -R
    I

    P
    T

    S
    D

    -R
    I/

    (I
    E

    S
    )

    151

    (c
    on

    ti
    nu

    ed
    )

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Ta
    bl

    e
    1

    (c
    on

    ti
    nu

    ed
    )

    S
    am

    pl
    e

    S
    iz

    e
    of

    Ty
    pe

    o
    f

    Tr
    au

    m
    at

    iz
    ed

    C
    om

    pa
    ri

    so
    n

    T
    im

    e
    C

    ur
    re

    nt
    o

    r
    D

    ia
    gn

    os
    ti

    c
    A

    ut
    ho

    r
    S

    am
    pl

    e
    Yo

    ut
    h

    G
    ro

    up
    s

    F
    ra

    m
    e

    L
    if

    et
    im

    e
    In

    fo
    rm

    an
    ts

    In
    st

    ru
    m

    en
    ts

    D
    av

    is
    s

    et
    a

    l.
    (2

    00
    0)

    de
    V

    ri
    es

    e
    t

    al
    .

    (1
    99

    9)

    D
    i

    G
    al

    lo
    ,

    B
    ar

    to
    n,

    &
    P

    ar
    ry

    -J
    on

    es
    (1

    99
    7)

    e

    D
    yb

    ,
    H

    ol
    en

    ,
    B

    ra
    en

    ne
    ,

    In
    dr

    ed
    av

    ik
    ,

    &
    A

    ar
    se

    th
    (

    20
    03

    )

    G
    er

    ri
    ng

    e
    t

    al
    .

    (2
    00

    2)

    K
    as

    sa
    m

    -A
    da

    m
    s

    &
    W

    in
    st

    on
    (

    20
    04

    )f

    K
    ep

    pe
    l-

    B
    en

    so
    n,

    O
    lle

    nd
    ic

    k,
    &

    B
    en

    so
    n

    (2
    00

    2)

    L
    ev

    i,
    D

    ro
    ta

    r,
    Ye

    at
    es

    ,
    &

    T
    ay

    lo
    r

    (1
    99

    9)

    M
    ax

    e
    t

    al
    .

    (1
    99

    8)

    M
    at

    he
    r,

    Ta
    te

    ,
    &

    H
    an

    na
    n

    (2
    00

    3)

    M
    cD

    er
    m

    ot
    t

    &
    C

    vi
    ta

    no
    vi

    ch
    (2

    00
    0)

    A
    cc

    id
    en

    ta
    l

    in
    ju

    ri
    es

    M
    VA

    M
    VA

    M
    VA

    C
    lo

    se
    d

    he
    ad

    i
    nj

    ur
    y

    M
    VA

    M
    VA

    S
    ev

    er
    e

    an
    d

    m
    od

    er
    at

    e
    T

    B
    I

    T
    B

    I

    M
    VA

    /(
    T

    B
    I)

    M
    VA

    48 10
    2

    57 16 95 17
    7

    50 ~
    71

    44 43 26

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    T
    B

    I
    vs

    .
    or

    th
    op

    ed
    ic

    i
    nj

    ur
    y

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    ;
    T

    B
    I

    vs
    .

    no
    n-

    T
    B

    I

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P P P P P P R P P P P

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    ?

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    ?

    C
    ur

    re
    nt

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    Pa
    re

    nt

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    U
    nc

    le
    ar

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    A

    P
    S

    -C
    A

    /
    P

    C
    L

    -C
    /P

    R

    P
    C

    L

    P
    T

    S
    D

    -R
    I/

    R
    IE

    S

    C
    P

    T
    S

    -R
    I

    D
    IC

    A

    C
    A

    P
    S

    -C
    A

    D
    IC

    A
    -R

    -C
    ;

    D
    IC

    A
    -R

    -P

    C
    P

    T
    S

    D
    -R

    I/
    P

    T
    S

    S

    K
    -S

    A
    D

    S

    C
    P

    T
    S

    -R
    I

    P
    T

    S
    D

    -R
    I

    152

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    M
    ir

    za
    ,

    B
    ha

    dr
    in

    at
    h,

    G
    oo

    dy
    er

    ,
    &

    G
    ilm

    ou
    r

    (1
    99

    8)

    S
    ax

    e,
    S

    to
    dd

    ar
    d,

    &
    S

    he
    ri

    da
    n

    (1
    99

    8)
    g

    S
    ta

    lla
    rd

    ,
    Ve

    lle
    m

    an
    ,

    &
    B

    al
    dw

    in
    (

    19
    98

    )h

    S
    to

    dd
    ar

    d,
    N

    or
    m

    an
    ,

    M
    ur

    ph
    y,

    &
    B

    ea
    rd

    sl
    ee

    (
    19

    89
    )i

    Zi
    nk

    &
    M

    cC
    ai

    n
    (2

    00
    3)

    M
    VA

    B
    ur

    ns

    M
    VA

    B
    ur

    ns

    M
    VA

    11
    9

    72 11
    9

    30 14
    3

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    S
    po

    rt
    s

    in
    ju

    ri
    es

    ;
    M

    V

    A
    P

    T
    S

    D
    v

    s.
    M

    VA
    n

    on
    -P

    T
    S

    D

    P
    T

    S
    D

    v
    s.

    n
    or

    m
    al

    c
    on

    tr
    ol

    s

    P
    T

    S
    D

    v
    s.

    n
    on

    -P
    T

    S
    D

    P P P R P

    U
    nc

    le
    ar

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    C
    ur

    re
    nt

    a
    nd

    L
    if

    et
    im

    e

    C
    ur

    re
    nt

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld

    C
    hi

    ld

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    C
    hi

    ld
    a

    nd
    P

    ar
    en

    t

    P
    T

    S
    D

    -R
    I/

    K
    -S

    A
    D

    S

    C
    P

    T
    S

    D
    -R

    I

    C
    A

    P
    S

    -C
    A

    D
    IC

    A
    /S

    C
    ID

    D
    IC

    A
    -R

    N
    O

    T
    E

    : P
    T

    S
    D

    =
    p

    os
    tt

    ra
    um

    at
    ic

    s
    tr

    es
    s

    di
    so

    rd
    er

    ; P
    T

    S
    D

    -R
    I

    =
    P

    os
    tt

    ra
    um

    at
    ic

    S
    tr

    es
    s

    D
    is

    or
    de

    r-
    R

    ea
    ct

    io
    n

    In
    de

    x;
    P

    S
    S

    =
    P

    T
    S

    O
    S

    ym
    pt

    om
    S

    ca
    le

    ;

    I
    E

    S
    =

    I
    m

    pa
    ct

    o
    f

    E
    ve

    nt
    S

    ca
    le

    ; T
    S

    C
    =

    T
    ra

    um
    a

    S
    ym

    pt
    om

    c
    he

    ck
    lis

    t;
    C

    P
    T

    S
    D

    -R
    I

    =
    C

    hi
    ld

    P
    os

    tt
    ra

    um
    at

    ic
    S

    tr
    es

    s
    D

    is
    or

    de
    r-

    R
    ea

    ct
    io

    n
    In

    de
    x;

    I
    E

    S
    -R

    =
    I

    m
    pa

    ct
    o

    f
    E

    ve
    nt

    s
    S

    ca
    le

    -R
    ev

    is
    ed

    ;
    S

    C
    ID

    =
    S

    tr
    uc

    tu
    re

    d
    C

    lin
    ic

    al
    In

    te
    rv

    ie
    w

    f
    or

    D
    S

    M
    -I

    V;
    D

    IS
    =

    D
    ia

    gn
    os

    ti
    c

    In
    te

    rv
    ie

    w
    S

    ch
    ed

    ul
    e;

    U
    C

    L
    A

    -P
    T

    S
    D

    -R
    I

    =
    U

    C
    L

    A
    P

    os
    tt

    ra
    um

    at
    ic

    S
    tr

    es
    s

    D
    is

    or
    de

    r-
    R

    ea
    ct

    io
    n

    In
    de

    x;
    C

    P
    T

    S
    -R

    I
    =

    C
    hi

    ld
    P

    os
    tt

    ra
    um

    at
    ic

    S
    tr

    es
    s

    R
    ea

    ct
    io

    n
    In

    de
    x;

    C
    A

    P
    S

    -C
    A

    =
    C

    lin
    ic

    ia
    n-

    A
    dm

    in
    is

    te
    re

    d
    P

    T
    S

    D
    S

    ca
    le

    (
    C

    hi
    ld

    a
    nd

    A
    do

    le
    sc

    en
    t

    ve
    rs

    io
    n)

    ;
    P

    C
    L

    =
    P

    os
    tt

    ra
    um

    at
    ic

    S
    tr

    es
    s

    D
    is

    or
    de

    r
    C

    he
    ck

    lis
    t;

    P
    C

    L
    -C

    =
    Po

    st
    tr

    au
    m

    at
    ic

    S
    tr

    es
    s

    D
    is

    or
    de

    r
    C

    he
    ck

    lis
    t-

    C
    iv

    ili
    an

    V
    er

    si
    on

    ;
    R

    IE
    S

    =
    R

    ev
    is

    ed
    I

    m
    pa

    ct
    o

    f
    E

    ve
    nt

    S
    ca

    le
    ;

    D
    IC

    A
    =

    D
    ia

    gn
    os

    ti
    c

    In
    te

    rv
    ie

    w
    f

    or
    C

    hi
    ld

    re
    n

    an
    d

    A
    do

    le
    sc

    en
    ts

    ;
    D

    IC
    A

    -R
    -C

    =
    D

    ia
    gn

    os
    ti

    c
    In

    te
    rv

    ie
    w

    f
    or

    C
    hi

    ld
    re

    n
    an

    d
    A

    do
    le

    sc
    en

    ts
    -R

    ev
    is

    ed
    -C

    hi
    ld

    V
    er

    si
    on

    ; D
    IC

    A
    -R

    -P
    =

    D
    ia

    gn
    os

    ti
    c

    In
    te

    rv
    ie

    w
    f

    or
    C

    hi
    ld

    re
    n

    an
    d

    A
    do

    le
    sc

    en
    ts

    -R
    ev

    is
    ed

    -P
    ar

    en
    t

    Ve
    rs

    io
    n;

    K
    -S

    A
    D

    S
    =

    S
    ch

    ed
    ul

    e
    fo

    r
    A

    ff
    ec

    ti
    ve

    D
    is

    or
    de

    rs
    a

    nd
    S

    ch
    iz

    op
    hr

    en
    ia

    f
    or

    S
    ch

    oo
    l-

    A
    ge

    C
    hi

    ld
    re

    n.
    a.

    B
    as

    ed
    o

    n
    da

    ta
    f

    ro
    m

    G
    er

    ri
    ng

    e
    t

    al
    .

    (2
    00

    2)
    ;

    K
    ep

    pe
    l-

    B
    en

    so
    n,

    O
    lle

    nd
    ic

    k,
    a

    nd
    B

    en
    so

    n
    (2

    00
    2)

    .
    b.

    B
    as

    ed
    o

    n
    da

    ta
    f

    ro
    m

    G
    er

    ri
    ng

    e
    t

    al
    .

    (2
    00

    2)
    ;

    K
    ep

    pe
    l-

    B
    en

    so
    n,

    O
    lle

    nd
    ic

    k,
    a

    nd
    B

    en
    so

    n
    (2

    00
    2)

    ;
    M

    at
    he

    r,
    Ta

    te
    ,

    an
    d

    H
    an

    na
    n

    (2
    00

    3)
    ;

    S
    ta

    lla
    rd

    ,
    Ve

    lle
    m

    an
    ,

    an
    d

    B
    al

    dw
    in

    (
    19

    98
    ).

    c.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    G

    er
    ri

    ng
    e

    t
    al

    .
    (2

    00
    2)

    ;
    M

    at
    he

    r,
    Ta

    te
    ,

    an
    d

    H
    an

    na
    n

    (2
    00

    3)
    ;

    S
    ta

    lla
    rd

    ,
    Ve

    lle
    m

    an
    ,

    an
    d

    B
    al

    dw
    in

    (
    19

    98
    ).

    d.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    D

    av
    is

    s
    et

    a
    l.

    (2
    00

    0)
    ;

    K
    as

    sa
    m

    -A
    da

    m
    s

    an
    d

    W
    in

    st
    on

    (
    20

    04
    ).

    e.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    B

    ry
    an

    t,
    M

    ay
    ou

    ,
    W

    ig
    gs

    ,
    E

    hl
    er

    s,
    a

    nd
    S

    to
    re

    s
    (2

    00
    4)

    ;
    S

    ax
    e,

    S
    to

    dd
    ar

    d,
    a

    nd
    S

    he
    ri

    da
    n

    (1
    99

    8)
    .

    f.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    A

    ar
    on

    ,
    Za

    gl
    ul

    ,
    an

    d
    E

    m
    er

    y
    (1

    99
    9)

    ;
    M

    at
    he

    r,
    Ta

    te
    ,

    an
    d

    H
    an

    na
    n

    (2
    00

    3)
    ;

    M
    cD

    er
    m

    ot
    t

    an
    d

    C
    vi

    ta
    no

    vi
    ch

    (
    20

    00
    ).

    g.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    G

    er
    ri

    ng
    e

    t
    al

    .
    (2

    00
    2)

    ;
    S

    ta
    lla

    rd
    ,

    Ve
    lle

    m
    an

    ,
    an

    d
    B

    al
    dw

    in
    (

    19
    98

    ).
    h.

    B
    as

    ed
    o

    n
    da

    ta
    f

    ro
    m

    B
    ry

    an
    t,

    M
    ay

    ou
    ,

    W
    ig

    gs
    ,

    E
    hl

    er
    s,

    a
    nd

    S
    to

    re
    s

    (2
    00

    4)
    ;

    M
    cD

    er
    m

    ot
    t

    an
    d

    C
    vi

    ta
    no

    vi
    ch

    (
    20

    00
    );

    S
    ta

    lla
    rd

    ,
    Ve

    lle
    m

    an
    ,

    an
    d

    B
    al

    dw
    in

    (
    19

    98
    ).

    i.
    B

    as
    ed

    o
    n

    da
    ta

    f
    ro

    m
    D

    av
    is

    s
    et

    a
    l.

    (2
    00

    0)
    ;

    G
    er

    ri
    ng

    e
    t

    al
    .

    (2
    00

    2)
    ;

    K
    ep

    pe
    l-

    B
    en

    so
    n,

    O
    lle

    nd
    ic

    k,
    a

    nd
    B

    en
    so

    n
    (2

    00
    2)

    ;
    S

    ax
    e,

    S
    to

    dd
    ar

    d,
    a

    nd
    S

    he
    ri

    da
    n

    (1
    99

    8)
    ;

    S
    ta

    lla
    rd

    ,
    Ve

    lle
    m

    an
    ,

    an
    d

    B
    al

    dw
    in

    (
    19

    98
    ).

    153

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    154 Traumatology / Vol. 12, No. 2, June 2006

    criteria. Many research and clinical settings tend to
    interpret scores in these ranges as indicative of clin-
    ically meaningful levels of PTSS or PTSD (e.g., Levi
    et al., 1999). If multiple time point assessments
    were conducted, the estimates closest to the trauma
    (within at least 1 month to allow for the emergence
    of PTSD symptoms) were included. Any follow-up
    data related to the trauma and rates of PTSD were
    included when it was available.

    Subthreshold PTSD has been defined differently
    within the literature, such as the endorsement of at
    least one, two, or three PTSSs (Marshall et al.,
    2001) or of at least two of the three symptom clus-
    ters (Aaron et al., 1999). The authors employed the
    criteria of endorsing at least two PTSSs to meet cri-
    teria for subthreshold PTSD, as this was basically
    consistent with the methodologies noted above. Also
    important to note is that there was often a great deal
    of variability in the comparison groups used in the
    studies reviewed in this article (e.g., normal con-
    trols, comparing those developing PTSD vs. those
    who do not after the experience of a trauma; see
    Table 1). As such, the various PTSD predictors and
    correlates described within this article are to be
    interpreted cautiously. Because very few studies
    examined similar variables that related to either of
    the traumas, a predictor needed to be examined in at
    least two independent studies for inclusion in the
    current analysis (per Rosenthal, 1991).

    Data Analytic Plan

    For each study that met inclusion criteria, the
    authors coded descriptive information including the
    mean age of youth at the time of PTSD assessment,
    percentage of the sample that was female, mean
    PTSD, and partial PTSD prevalence rates along with
    other study characteristics, such as type of trauma
    and informant of PTSS. Next, predictors for the
    development of PTSD among youth exposed to ill-
    nesses and injuries were evaluated. Studies that uti-
    lized between-group designs typically reported t, F,
    and chi-square statistics, whereas studies using cor-
    relational designs reported either Pearson r or phi
    statistics. Per Rosenthal (1991, 1994) and Hedges
    and Olkin (1985), all of the statistics were converted
    to Cohen’s d to yield a single common measure of
    effect size. The use of effect size statistics for both
    group differences and correlational data is consistent
    with current standard statistical practices (Lipsey &
    Wilson, 2001). Conventional social sciences research

    generally interprets Cohen’s d effect size values as .2
    for small, .5 for medium, and .8 for large effects,
    with higher d values indicating a stronger relation-
    ship with PTSD.

    Q statistics were examined to test for homogene-
    ity among the effect sizes associated with any given
    predictor. In general, significant Q statistics indicate
    that the variability among effect sizes is greater than
    what would result from subject-level sampling error
    alone. As such, for those predictors with significant
    Q statistics, the authors chose to report effect sizes
    individually (rather than as part of an aggregate). This
    approach is consistent with statistical standards (e.g.,
    Durlak, 1995). Moreover, because of the exploratory
    nature of the current manuscript, this approach
    seemed preferable than simply utilizing a random
    effects model, which would have obscured the poten-
    tial significant differences between certain effect
    sizes. For those predictors in which the Q statistics
    were not significant, the effect sizes were averaged
    (aggregated) and weighted by the sample sizes asso-
    ciated with the predictors.

    In accordance with the rules described above,
    several predictors and correlates were culled from the
    literature. For both groups, the associations between
    mean age of youth, gender rates, and PTSD preva-
    lence rates were computed as was the lag between
    the trauma and the appraisal of symptoms (see Table 2).
    For youth experiencing injuries, 8 unique predictors
    were identified: (a) socioeconomic status (SES),
    (b) Internalizing scale on the Child Behavior Checklist
    (CBCL; Achenbach, 1991), (c) comorbid depressive
    symptoms, (d) comorbid anxious symptoms, (e) comorbid
    dissociation, (f) acute stress disorder (ASD), (g) social
    impairment, and (h) appraisal of trauma severity
    (and/or life appraisal). For youth with illnesses, the
    years since active treatment, appraisal of trauma
    severity (and/or life appraisal), and social support
    emerged as important PTSD predictors (see Table 3).

    Results

    Injury

    As Table 2 demonstrates, 11 of the 18 studies (61%)
    involved MVAs, whereas the remainder included
    participants experiencing burns (n = 2), TBIs or
    closed-head injuries (n = 3), and a variety of physical
    injuries (n = 2). The mean age of participants was
    11.3 years (SD = 1.41 years), and 41% of the studies’
    participants were female (weighted by sample size).

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Meta-Analysis of PTSD in Youth / Kahana et al. 155

    The average sample size was approximately 70.39
    (SD = 46.38) participants. Caucasians composed the
    large majority of the study samples at 70%, whereas
    African American and Hispanic populations constituted

    24% and 5%, respectively, of the samples. Finally,
    the average lag time between the occurrence of the
    trauma and the assessment of PTSS/PTSD was 4.97
    (SD = 3.77) months.

    Table 2. Demographics and Prevalence Rates of PTSD Among Pediatric Injury and Illness Populations

    Injury Population (n = 18 studies) Weighted Mean Illness Population (n = 8 studies) Weighted Mean

    Age 11.30 (1.41) Mean age 13.39 (2.67)
    Percentage female 41.01 Percentage female 50.29
    PTSD prevalence rates 19.82 (9.94) PTSD prevalence rates 12.04 (7.87)
    Partial PTSD rates 25.03 (8.06) Partial PTSD rates 37.58 (11.98)
    Sample size 70.39 (46.38) Sample size 61.75 (51.96)

    PTSD correlates Pearson’s r PTSD correlates Pearson’s r
    Mean age .04 Mean age –.48
    Gender (female) .09 Gender .04
    Gender (and partial PTSD) .50 Gender (and partial PTSD) .88
    Trauma and assessment lag –.26 Years since active treatment –.63*

    NOTE: PTSD = posttraumatic stress disorder.
    *p < .05.

    Table 3. Predictors of PTSD Among Pediatric Injury and Illness Populations

    Cohen’s d Cohen’s d

    SESa –0.45 Appraisalj .39
    CBCL Internalizing Scaleb 1.03 Social Supportk .14
    Depressive Symptomsc 1.06, 1.09, 1.57
    Anxious Symptomsd 0.90, 0.90, 1.64, 1.96
    Dissociatione 3.53, 1.19
    Acute Stress Disorderf 0.94, 1.35
    Appraisal of Life Threatg 0.82
    Trauma Severityh –0.39, 0.19, 0.44, 0.85, 1.32
    Social Impairmenti 0.41

    NOTE: Cohen’s d effect size values are d = .2 for small, .5 for medium, and .8 for large effects. The effect sizes for Depressive
    Symptoms, Anxious Symptoms, Dissociation, Acute Stress Disorder, and Trauma Severity are presented separately (instead of as part
    of a weighted mean), as Q statistics indicated a significant amount of heterogeneity between them. PTSD = posttraumatic stress dis-
    order; SES = socioeconomic status; CBCL = Child Behavior Checklist.
    a. Based on data from Gerring et al. (2002); Keppel-Benson, Ollendick, and Benson (2002).
    b. Based on data from Aaron, Zaglul, and Emery (1999); Mather, Tate, and Hannan (2003); McDermott and Cvitanovich (2000).
    c. Based on data from Gerring et al. (2002); Mather, Tate, and Hannan (2003); Stallard, Velleman, and Baldwin (1998).
    d. Based on data from Gerring et al. (2002); Keppel-Benson, Ollendick, and Benson (2002); Mather, Tate, and Hannan (2003);
    Stallard, Velleman, and Baldwin (1998).
    e. Based on data from Bryant, Mayou, Wiggs, Ehlers, and Stores (2004); Saxe, Stoddard, and Sheridan (1998).
    f. Based on data from Daviss et al. (2000); Kassam-Adams and Winston (2004).
    g. Based on data from Bryant, Mayou, Wiggs, Ehlers, and Stores (2004); McDermott and Cvitanovich (2000); Stallard, Velleman,
    and Baldwin (1998).
    h. Based on data from Daviss et al. (2000); Gerring et al. (2002); Keppel-Benson, Ollendick, and Benson (2002); Saxe, Stoddard,
    and Sheridan (1998); Stallard, Velleman, and Baldwin (1998).
    i. Based on data from Gerring et al. (2002); Stallard, Velleman, and Baldwin (1998).
    j. Based on data from Brown, Madan-Swain, and Lambert (2003); Shemesh et al. (2000); Stuber et al. (1996).
    k. Based on data from Brown, Madan-Swain, and Lambert (2003); Pelcovitz et al. (1998).

    1. One study (Brown, Madan-Swain, & Lambert, 2003) contained a few childhood cancer survivors who were 23 years old. Despite
    this, the study was included because it was mostly composed of adolescents.
    2. Several studies were excluded because the injury versus illness categories were not differentiated (Balluffi et al., 2004; Landolt,
    Vollrath, Ribi, Gnehm, & Stennhauser, 2003).

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    156 Traumatology / Vol. 12, No. 2, June 2006

    Average PTSD prevalence rates ranged from 0%
    to 37.5%, with a mean of 19.82% (SD = 9.94)
    weighted by sample size. Of the 18 studies involving
    injuries, more than half (n = 11) provided enough
    information to calculate that 25% of the samples
    met criteria for partial PTSD. With respect to spe-
    cific clusters, 50.47%, 32.53%, and 17.90% of the
    participants endorsed sufficient symptoms to meet
    criteria for the re-experiencing, arousal, and avoid-
    ance clusters, respectively. A paired sample t test
    revealed that all these rates were significantly different
    from each other (i.e., arousal vs. avoidance, t(4) = 3.66,
    p < .05; reexperiencing vs. avoidance, t(4) = 10.13, p < .005; reexperiencing vs. arousal, t(4) = 3.13, p < .05). It is conceivable that the greater number of youth meeting criteria for the re-experiencing clus- ter is due to the fact that only 1 re-experiencing symptom needs to be endorsed to meet diagnostic criteria. Indeed, of the three studies that reported percentages of specific symptoms, the re-experiencing symptoms of intrusive recollections and psychological exposure to trauma-related cues were among the most highly endorsed symptoms, with weighted percents of 61.46% and 51.32%, respectively.

    Among youth experiencing injuries, PTSD
    prevalence rates were not strongly associated with
    mean age of youth, r(15) = .04, n.s., or female gen-
    der, r(15) = .09, n.s. (see Table 2). However, females
    exhibited relatively high rates of subthreshold PTSD
    at r(10) = .50, n.s. There was a statistical trend for
    PTSD prevalence rates to be inversely related with
    the lag between the occurrence of the trauma and
    the assessment of symptoms at r(16) = –.26, p < .10. This suggests that injured youth tended to exhibit lower rates of PTSD as more time lapsed between the experience of the trauma and the assessment of PTSD.

    Among youth experiencing injuries, SES was a
    significant, though small to moderate, predictor of
    PTSS (Q = 3.35 [1], n.s.; mean weighted d = –.45
    [95% confidence interval (CI) = –0.30 to –0.60]).
    This suggests that youth from a lower SES were
    somewhat more likely to develop PTSSs after expe-
    riencing an injury. PTSS and PTSD were also often
    related to the experience of other internalizing dis-
    orders. Specifically, injured children with PTSSs
    tended to endorse significantly higher clinical eleva-
    tions on the CBCL Internalizing scale (Q = 3.55 [2],
    n.s.; mean weighted d = 1.03 [95% CI = 0.85 to
    1.21]) as well as a greater number of anxiety symp-
    toms (Q =58.85 [3], p < .0001; ds = 0.90, 0.90, 1.64, and 1.96, respectively) as compared to youth

    who sustained injuries but who did not endorse
    PTSSs. Similarly, the endorsement of significant
    depressive symptoms was also strongly related to
    increased PTSS rates (Q =18.26 [2], p < .0005; ds = 1.06, 1.09, and 1.57), with large to very large effect sizes indicating considerable comorbidity between PTSD and depressive symptoms.

    With two effect size estimates in the large to very
    large range, dissociation served as a very robust pre-
    dictor of PTSD diagnosis (Q = 65.34 [1], p < .0001; ds = 3.53 and 1.19, respectively) among youth sus- taining injuries. This result is logical, as dissociation can often be a core symptom of PTSD. ASD was also a strong predictor of PTSSs among injured youth, with two reported effect sizes in the large range (Q = 6.34 [1], p < .05; ds = 0.94 and 1.35, respectively). Finally, injured youth with PTSD demonstrated small to moderate impairments in social functioning (Q = .08 [1], n.s.; mean weighted d = .41 [95% CI = 0.39 to 0.43]).

    A particularly interesting finding is that the
    individual’s subjective appraisal of the life threat
    involved in the injury was a robust predictor of
    PTSD (Q = 4.78 [2], n.s.; mean weighted d = .82
    [95% CI = 0.67 to 0.97]). This indicates that among
    injured youth, the perception of threat to one’s life
    or physical integrity was usually strongly related to
    the development of PTSSs. Somewhat concordant
    with this finding was the relationship between
    PTSSs and the vaguely defined “trauma severity”
    construct, which was rated either by the clinician,
    parent, or youth. Various effect sizes were reported
    for this association (Q = 85.58 [4], p < .0001; ds = –0.39, 0.19, 0.44, 0.85, and 1.32), with some in the small to moderate range and others in the large to very large range. It is likely that the varied perspec- tives of different raters contributed, at least in part, to the variability in the effect sizes. For example, a clinician might focus on the more objective severity (e.g., acuity, intensity, length of exposure to trauma) associated with an injury, whereas the parents and child will likely appraise the trauma severity more subjectively (e.g., involvement of any pain). In addi- tion, because severity was defined somewhat differ- ently between studies, it was difficult to identify meaningful trends regarding specific informants attached to the varied effect sizes.

    Illness

    Six of the eight studies involved pediatric or adolescent
    cancer survivors, whereas the other two focused on

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    pediatric liver transplantees. The mean age of partic-
    ipants was 13.39 years (SD = 2.67 years), and about
    half (50.29%) of the participants were female. The
    average sample size for youth experiencing illnesses
    was 61.75 (SD = 51.96) participants. More than
    three quarters (75%) of the study participants were
    Caucasian, and the remaining 25% were divided
    among African Americans, Hispanics, Asians, and
    Other. Finally, the mean number of years since active
    treatment had ended and the assessment of PTSD/
    PTSS was 3.65 (SD = 2.04) years.

    Among ill youth, the average PTSD prevalence
    rates ranged from 0% to 32%, with a mean of 12.04%
    (SD = 7.87) weighted by sample size. Insufficient
    data precluded providing information about the
    symptom clusters among youth experiencing ill-
    nesses. Among these youth, female gender was not
    associated with PTSD prevalence rates, r(6) = .04,
    n.s. However, the mean age of the youth appeared to
    be inversely related with PTSD prevalence, r(7) =
    –.48, n.s., suggesting that the younger the youth, the
    more likely they were to display PTSSs. Subthreshold
    PTSD rates for ill youth were fairly high at 37.58%
    (SD = 11.98), with females tending to exhibit rela-
    tively high rates at r(3) = .88, n.s.

    Of the six studies that described youth who had
    been survivors of (as compared to youth still under-
    going) illnesses or procedures, there was a robust
    and statistically significant association between
    PTSD prevalence rates and the time since youth had
    finished their active treatments and/or medical pro-
    cedures, r(6) = –.63, p < .05. That is, youth exhib- ited lower rates of PTSD when more time had lapsed between the illness/procedure and the assessment of their trauma symptoms. This suggests that among youth experiencing illnesses, PTSD is likely to occur shortly following traumatic exposure (with delayed onset less common), or alternatively, that trauma symptoms diminish over time. Because most studies that involve ill youth do not pinpoint the time when PTSSs have emerged, and evaluate current as com- pared to past symptoms, the data more likely indi- cate that symptoms decrease with time, and, thus measure chronicity.

    The association between perceived severity of
    trauma (and/or life threat) and PTSSs was small to
    moderate (Q = 5.47 [2], n.s.; mean weighted d = .39;
    95% CI = 0.16 to 0.62). Three studies contributed
    to this finding; one study involved a clinician’s
    assessment and the other two were made by the
    youth. Finally, the social support construct (broadly

    defined as support from one’s family and friends)
    was investigated within two pediatric illness studies.
    Among ill youth, social support exhibited a relatively
    small relationship with the development of PTSS
    (Q = .08 [1], n.s.; mean weighted d = .14; 95% CI =
    0.11 to 0.17). This is surprising, as past literature
    has noted the importance of social support as a
    buffer against the development of PTSD. It is possi-
    ble that this finding is an artifact of the protracted
    assessment lag that occurred for these youth.

    Differences Between the
    Groups on Prevalence Rates
    and Time of Assessment

    Several important differences between the injury
    and illness groups emerged in this review. First,
    a chi-square test of proportions indicated that the
    prevalence rates of PTSD differed significantly
    between youth experiencing injury versus illness
    (χ2 = 14.45 [1], p < .0005). Injured youth exhibited a mean of 19.82% (SD = 9.94), whereas ill youth displayed a mean of 12.04% (SD = 7.87). Another important finding is that the mean effect size differ- ences between the groups on the perceived life threat and/or assessment of trauma severity variable were approaching statistical significance (difference between independent ds; z = 1.90, p = .06), with children in the injury group endorsing higher rates of perceived trauma severity and/or life threat. This disparity might be attributed to significant differ- ences in the mean lag of PTSD assessment for youth in both groups (t = 8.55 [1,215], p < .0001). Specifically, youth experiencing illnesses were often followed up years after the diagnosis and treatments for their illness, whereas youth exposed to injuries were often assessed months after their experienced trauma.

    The availability of Time 2 PTSD assessment data
    in a subset of studies involving injured youth made it
    possible to formally examine this hypothesis. Nine of
    the 18 studies (50%) measured PTSS and PTSD
    prevalence rates at multiple time points, allowing for
    a comparison of PTSD prevalence rates at different
    time points among the same trauma group. Within
    these 9 studies, Time 1 assessments occurred an
    average of 3.40 (SD = 3.38) months after the trauma
    occurrence, whereas Time 2 assessments occurred
    an average of 6.48 (SD = 2.64) months after the
    trauma. The mean PTSD prevalence rates of 24.61%
    (SD = 9.15) at Time 1 were significantly higher than

    Meta-Analysis of PTSD in Youth / Kahana et al. 157

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    the PTSD prevalence rates of 16.25% (SD = 9.77) at
    Time 2 (χ2 = 19.61 [1], p < .0005). This finding sup- ports the notion that delayed onset of PTSSs is not common and likely occurs only in a minority of injured youth. In addition, it appears that among injured youth, PTSSs subside with time, a pattern consonant with that manifested by ill youth.

    Discussion

    The goal of the current meta-analysis was to review
    and synthesize studies addressing PTSD in youth
    exposed to medical illness or injury. Several interest-
    ing findings emerged from this investigation. Among
    the most important is that the injury- and illness-
    related trauma literatures have not examined many
    similar or overlapping predictors of PTSD. The
    sparseness of replication data with respect to poten-
    tial predictors makes it difficult to investigate mean-
    ingful comparisons across studies, either between
    the phenomenology of PTSD or PTSD predictors.

    This review highlights that a minority of youth
    experiencing illnesses or injuries typically developed
    PTSD or PTSSs. The prevalence rates of PTSD
    appeared higher among youth experiencing injuries
    as compared to illness. However, it is crucial to high-
    light that this result might be the product of
    methodological artifact. Specifically, although some
    of the cancer and transplant studies followed chil-
    dren for a considerable number of years, follow-up
    periods for injured youth were shorter and more
    proximal to the traumatic event, thus resulting in a
    possible inflation of differences in PTSD rates
    between the groups. In fact, support for this notion
    comes from Time 2 assessment data among injured
    youth, where PTSD rates were significantly lower at
    follow-up as compared to the initial assessment.

    Age at the time of PTSD assessment appears to
    be more closely related to the development of
    PTSD/PTSSs among youth with illnesses as com-
    pared to those with injuries. Specifically, the older
    the ill youth, the less likely they were to exhibit sig-
    nificant PTSSs. This might be consistent with
    research suggesting that older youth possess more
    cognitive capacities to implement coping strategies
    after a trauma than do younger youth (Joseph,
    Brewin, Yule, & Williams, 1993). Alternatively, the
    relationship between mean age and PTSD rates
    within the illness group might also be the result of
    the time gap between the termination of active med-
    ical treatment and the evaluation of symptoms. That

    is, the lag between PTSD assessment and trauma
    occurrence may be masking the actual association
    between age and PTSD.

    Although females were more likely than males to
    display PTSSs (and subthreshold PTSD), the current
    meta-analysis indicated there did not appear to be
    significant gender differences in rates of diagnostic
    PTSD among youth experiencing injuries and ill-
    nesses. Although the adult literature consistently
    indicates that women are at higher risk for develop-
    ing PTSD following various traumas (Breslau et al.,
    1998; Kessler, Sonnega, Bromet, Hughes, & Nelson,
    1995), these data do not typically include medically
    ill samples and thus might not be the most appropriate
    for comparison with the current findings. Another
    explanation might be that the degree of trauma severity,
    whether subjective or objective, might have differed
    between males and females, thus leading to differ-
    ences in symptom rates. Although there are reported
    differences in exposure to various traumas among
    males and females, with males typically exposed to
    greater rates of community and physical violence
    (e.g., Jaycox et al., 2002) and females to greater rates
    of sexual abuse (e.g., Putnam, 2003), there is no lit-
    erature comparing the severity of experienced traumas
    between the genders.

    Internalizing symptoms, particularly those asso-
    ciated with depression and anxiety, appear to be
    highly comorbid with PTSD in injured youth. This is
    helpful clinical information, as it suggests that
    PTSSs will typically not occur in isolation from
    other psychiatric symptoms. This finding is consis-
    tent with research that reports high levels of comor-
    bidity among adults with PTSD (e.g., Kessler et al.,
    1995). Finally, although temporal order of onset is
    difficult to establish, it is possible that preexisting
    internalizing disorders, such as anxiety, serve as a
    diathesis that places people at higher risk for devel-
    oping PTSD after a trauma (Bradley, 2000).

    The appraisal of life threat and trauma severity
    is a construct that requires further study. There are
    many components to consider, including but not
    limited to, medical late effects, future necessary
    treatments, and other individuals directly affected
    by the trauma. The relationship between the appraisal
    of trauma severity and PTSD was moderate to large
    in both the illness and injury groups. However, given
    the ambiguity of the construct (i.e., is severity sim-
    ply a proxy for life threat?), it is difficult to make
    conclusive interpretations about these data. Future
    studies would be well served to integrate both objective

    158 Traumatology / Vol. 12, No. 2, June 2006

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Meta-Analysis of PTSD in Youth / Kahana et al. 159

    and subjective (e.g., including collateral family reports)
    trauma severity ratings to make comparisons between
    trauma groups.

    Research conducted to date has important
    implications for identifying those youth who are at
    risk for developing PTSD in response to illnesses or
    injuries. First, it is important for clinicians and
    researchers to assess uniform predictors and corre-
    lates, including appraisal of life threat, academic
    functioning, family functioning, social support, cog-
    nitive strategies, dissociation, and perceived levels of
    stress. It is only through replication by independent
    research groups that predictors will gain credibility.
    Further potential support for the role of these iden-
    tified factors can be useful to practitioners in assess-
    ing and preventing psychiatric symptoms and/or
    improving functioning. Perhaps with more conclu-
    sive research of predictors and moderators of PTSD
    with youth experiencing illnesses and injuries, clini-
    cians and programs serving these youth (such as
    schools and medical clinics) will have a better
    understanding of services and types of treatment
    that need to be provided.

    Second, studies should include more ethnically
    diverse youth, as the composition of many of the
    studied samples was predominantly Caucasian. Third,
    to facilitate comparisons across studies, researchers
    should clearly describe the rationale and methods of
    selecting populations, as understanding potential
    sample limitations is vital for drawing generalizations
    from study results. Fourth, to understand the famil-
    ial context of PTSD, family members affected by
    the trauma should be assessed for PTSD and other
    psychopathology. Finally, the recent literature has
    addressed the importance of posttraumatic growth in
    the context of an experienced trauma (Milam, Ritt-
    Olson, & Unger, 2004; Salter & Stallard, 2004). As
    such, assessing the resilience and strengths of youth
    undergoing a trauma would allow for a more com-
    prehensive understanding of the youth’s psychologi-
    cal functioning after the trauma.

    This study has several limitations. Various poten-
    tial predictors could not be analyzed because they
    were not included in a sufficient number of inde-
    pendent studies. Unfortunately, much of the pedi-
    atric trauma research has been hindered by a lack of
    measurement of uniform variables. Specifically, it
    appears that of the 11 variables for which effect
    sizes were calculated, 5 of them only included infor-
    mation from only two independent studies. In addi-
    tion, the literature regarding illness (with only eight
    studies meeting criteria and two predictors) may be

    less advanced than the literature pertaining to
    injuries (with 18 studies and eight identified predic-
    tors). Moreover, several studies reported statistics
    (e.g., multiple regressions) about PTSD predictors
    that were not included in this review because the
    values depended on the constellation of covariates
    used in the original study. Another significant limi-
    tation is that the comparison groups (e.g., normal
    controls, all traumatized) often differed between the
    injury and illness samples, rendering comparisons
    across the trauma groups quite difficult. Finally, null
    findings not reported in the literature might limit
    the generalizability of the findings from this review.

    In summary, there is much more research
    that needs to be conducted before conclusive state-
    ments can be made about the predictors for the
    development of PTSD in trauma-exposed youth.
    Despite the limitations discussed above, data sug-
    gest that among injured youth, SES and social
    impairment were small to moderate correlates of
    PTSD, whereas depressive and anxious symptoms,
    dissociation, ASD, and the appraisal of trauma
    severity and life threat served as robust predictors
    of PTSD. Among ill youth, social support and the
    appraisal of illness severity and life threat emerged
    as small to moderate predictors of PTSSs. Rather
    than constituting a rigid set of predictors, these fac-
    tors should be conceptualized as a rough heuristic
    that clinicians can employ when appraising trauma-
    exposed youth. Accordingly, clinicians should closely
    monitor at-risk individuals who exhibit many of the
    predictors identified in this article in an attempt to
    prophylactically address psychiatric symptoms before
    they become chronic.

    Acknowledgments

    The authors would like to thank Sheridan Stull, BA,
    Judith Geraci, BA, Jennifer Goodpaster, BA, and
    Kristen Walter, BA, for their aid in the collection of
    articles included in the current manuscript. In addi-
    tion, the authors wish to express appreciation to the
    Lance Armstrong Center for Survivors of Pediatric
    Illness for their continued support and funding.

    References

    *Studies preceded by an asterisk were included in the meta-
    analysis.

    *Aaron, J., Zaglul, H., & Emery, R. E. (1999). Posttraumatic
    stress in children following acute physical injury. Journal
    of Pediatric Psychology, 24, 335-343.

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Achenbach, T. M. (1991). Manual for the Child Behavior
    Checklist/4-18 and 1991 profile. Burlington: University of
    Vermont, Department of Psychiatry.

    American Psychiatric Association. (2001). Diagnostic and
    statistical manual of mental disorders (4th ed.). Washington,
    DC: Author.

    Balluffi, A., Kassam-Adams, N., Kazak, A., Tucker, M.,
    Dominguez, T., & Helfaer, M. (2004). Traumatic stress in
    parents of children admitted to the pediatric intensive
    care unit. Pediatric Care Medicine, 5, 547-553.

    Berman, S. L., Kurtines, W. M., Silverman, W. K., & Serafini,
    L. T. (1996). The impact of exposure to crime and violence
    on urban youth. American Journal of Orthopsychiatry, 66,
    329-336.

    Bradley, S. J. (2000). Affect regulation and the development
    of psychopathology. New York: Guilford.

    Breslau, N., Kessler, R. C., Chilcoat, H. D., Schultz, L. R., Davis,
    G. C., & Andreski, P. (1998). Trauma and posttraumatic stress
    disorder in the community: The 1996 Detroit Area Survey of
    Trauma. Archives of General Psychiatry, 55, 626-632.

    *Brown, R. T., Madan-Swain, A., & Lambert, R. (2003).
    Posttraumatic stress symptoms in adolescent survivors of
    childhood cancer and their mothers. Journal of Traumatic
    Stress, 16, 309-318.

    *Bryant, B., Mayou, R., Wiggs, L., Ehlers, A., & Stores, G.
    (2004). Psychological consequences of road traffic acci-
    dents for children and their mothers. Psychological
    Medicine, 34, 335-346.

    *Butler, R. W., Rizzi, L. P., & Handwerger, B. A. (1996).
    Brief report: the assessment of posttraumatic stress disor-
    der in pediatric cancer patients and survivors. Journal of
    Pediatric Psychology, 21, 499-504.

    *Daviss, W. B., Mooney, D., Racusin, R., Ford, J. D.,
    Fleischer, A., & McHugo, G. J. (2000). Predicting post-
    traumatic stress after hospitalization for pediatric injury.
    Journal of the American Academy of Child and Adolescent
    Psychiatry, 39, 576-583.

    *de Vries, A. P., Kassam-Adams, N., Cnaan, A., Sherman-
    Slate, E., Gallagher, P. R., & Winston, F. K. (1999).
    Looking beyond the physical injury: posttraumatic stress
    disorder in children and parents after pediatric traffic
    injury. Pediatrics, 104, 1293-1299.

    *Di Gallo, A., Barton, J., & Parry-Jones, W. L. (1997). Road
    traffic accidents: Early psychological consequences in
    children and adolescents. British Journal of Psychiatry,
    170, 358-362.

    Durlak, J. A. (1995). Understanding meta-analysis. In L. A.
    Grimm & P. R. Yarnold (Eds.), Reading and understanding
    multivariate statistics (pp. 319-352). Washington, DC:
    American Psychological Association.

    *Dyb, G., Holen, A., Braenne, K., Indredavik, M. S., &
    Aarseth, J. (2003). Parent-child discrepancy in reporting
    children’s post-traumatic stress reactions after a traffic
    accident. Nordic Journal of Psychiatry, 57, 339-344.

    *Gerring, J. P., Slomine, B., Vasa, R. A., Grados, M., Chen,
    A., Rising, W., et al. (2002). Clinical predictors of

    posttraumatic stress disorder after closed head injury in
    children. Journal of the American Academy of Child and
    Adolescent Psychiatry, 41, 157-165.

    Gleser, L. J., & Olkin, I. (1994). Stochastically dependent
    effect sizes. In H. Cooper & L. V. Hedges (Eds.), The
    handbook of research synthesis (pp. 339-355). New York:
    Russell Sage Foundation.

    Guyer, B., Hoyert, D. L., Martin, J. A., Ventura, S. J.,
    MacDorman, M. F., & Strobino, D. M. (1999). Annual sum-
    mary of vital statistics-1998. Pediatrics, 102, 1333-1349.

    Hedges, L. V., & Olkin, I. (1985). Statistical methods for
    meta-analysis. San Diego, CA: Academic Press.

    Jaycox, L. H., Stein, B. D., Kataoka, S. H., Wong, M., Fink,
    A., Escudero, P., et al. (2002). Violence exposure, post-
    traumatic stress disorder, and depressive symptoms
    among recent immigrant schoolchildren. Journal of the
    American Academy of Child and Adolescent Psychiatry, 41,
    1104-1110.

    Joseph, S. A., Brewin, C. R., Yule, W., & Williams, R. (1993).
    Causal attributions and post-traumatic stress in adolescents.
    Journal of Child Psychology and Psychiatry, 34, 247-253.

    *Kassam-Adams, N., & Winston, F. K. (2004). Predicting
    child PTSD: The relationship between acute stress disorder
    and PTSD in injured children. Journal of the American
    Academy of Child and Adolescent Psychiatry, 43, 403-411.

    *Kazak, A. E., Alderfer, M. A., Streisand, R., Simms, S.,
    Rourke, M. T., Barakat, L. P., et al. (2004). Treatment of
    posttraumatic stress symptoms in adolescent survivors of
    childhood cancer and their families: A randomized clinical
    trial. Journal of Family Psychology, 18, 493-504.

    *Kazak, A. E., Barakat, L. P., Meeske, K., Christakis, D.,
    Meadows, A. T., Casey, R., et al. (1997). Posttraumatic
    stress, family functioning, and social support in survivors of
    childhood leukemia and their mothers and fathers. Journal
    of Consulting and Clinical Psychology, 65, 120-129.

    *Keppel-Benson, J. M., Ollendick, T. H., & Benson, M. J.
    (2002). Post-traumatic stress in children following motor
    vehicle accidents. Journal of Child Psychology and
    Psychiatry, 43, 203-212.

    Kessler, R. C., Sonnega, A., Bromet, E., Hughes, M., &
    Nelson, C. B. (1995). Posttraumatic stress disorder in the
    National Comorbidity Survey. Archives of General
    Psychiatry, 52, 1048-1060.

    La Greca, A., Silverman, W. K., Vernberg, E. M., & Prinstein,
    M. J. (1996). Symptoms of posttraumatic stress in children
    after Hurricane Andrew: A prospective study. Journal of
    Consulting and Clinical Psychology, 64, 712-723.

    Landolt, M. A., Vollrath, M., Ribi, K., Gnehm, H. E., &
    Sennhauser, F. H. (2003). Incidence and associations of
    parental and child posttraumatic stress symptoms in pedi-
    atric patients. Journal of Child Psychology and Psychiatry,
    44, 1199-1207.

    *Levi, R. B., Drotar, D., Yeates, K. O., & Taylor, H. G.
    (1999). Posttraumatic stress symptoms in children fol-
    lowing orthopedic or traumatic brain injury. Journal of
    Clinical Child Psychology, 28, 232-243.

    160 Traumatology / Vol. 12, No. 2, June 2006

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Meta-Analysis of PTSD in Youth / Kahana et al. 161

    Lipsey, M. W., & Wilson, D. B. (2001). Practical meta-analysis.
    Thousand Oaks, CA: Sage.

    Marshall, R. D., Olfson, M., Hellman, F., Blanco, C.,
    Guardino, M., & Struening, E. L. (2001). Comorbidity,
    impairment, and suicidality in subthreshold PTSD.
    American Journal of Psychiatry, 158, 1467-1473.

    *Mather, F. J., Tate, R. L., & Hannan, T. J. (2003). Post-
    traumatic stress disorder in children following road traffic
    accidents: A comparison of those with and without mild
    traumatic brain injury. Brain Injury, 17, 1077-1087.

    *Max, J. E., Castillo, C. S., Robin, D. A., Lindgren, S. D.,
    Smith, W. L. Jr., Sato, Y., et al. (1998). Posttraumatic stress
    symptomatology after childhood traumatic brain injury.
    Journal of Nervous and Mental Disease, 186, 589-596.

    *McDermott, B. M., & Cvitanovich, A. (2000). Posttraumatic
    stress disorder and emotional problems in children fol-
    lowing motor vehicle accidents: An extended case series.
    Australian and New Zealand Journal of Psychiatry, 34,
    446-452.

    Milam, J. E., Ritt-Olson, A., & Unger, J. B. (2004).
    Posttraumatic growth among adolescents. Journal of
    Adolescent Research, 19, 192-204.

    *Mirza, K. A. H., Bhadrinath, B. R., Goodyer, I. M., &
    Gilmour, C. (1998). Post-traumatic stress disorder in chil-
    dren and adolescents following road traffic accidents.
    British Journal of Psychiatry, 170, 358-362.

    Olson, C. M., Rennie, D., Cook, D., Dickersin, K., Flanagin,
    A., Hogan, J. W., et al. (2002). Publication bias in edito-
    rial decision making. Journal of the American Medical
    Association, 287, 2825-2828.

    *Pelcovitz, D., Libov, B. G., Mandel, F., Kaplan, S.,
    Weinblatt, M., & Septimus, A. (1998). Posttraumatic
    stress disorder and family functioning in adolescent can-
    cer. Journal of Traumatic Stress, 11, 205-221.

    Public Health Policy Advisory Board. (1999). Health and the
    American child: Risks, trends, and priorities for the 21st
    century. Washington, DC: Author.

    Putnam, F. W. (2003). Ten-year research update review:
    child sexual abuse. Journal of the American Academy of
    Child and Adolescent Psychiatry, 42, 269-278.

    Reinherz, H. Z., Giaconia, R. M., Lefkowitz, E. S., Pakiz, B.,
    & Frost, A. K. (1993). Prevalence of psychiatric disorders
    in a community population of older adolescents. Journal
    of the American Academy of Child and Adolescent
    Psychiatry, 32, 369-377.

    Rosenthal, R. (1991). Meta-analytic procedures for social
    research (Rev. ed.). Newbury Park, CA: Sage.

    Rosenthal, R. (1994). Parametric measures of effect size. In
    H. Cooper & L. V. Hedges (Eds.), The handbook of research
    synthesis (pp. 231-244). New York: Russell Sage Foundation.

    Salter, E., & Stallard, P. (2004). Posttraumatic growth in
    child survivors of a road traffic accident. Journal of
    Traumatic Stress, 17, 335-340.

    *Saxe, G. N., Stoddard, F. J., & Sheridan, R. (1998). PTSD
    in children with burns: A longitudinal study. Burn Care &
    Rehabilitation, Part 2, S206.

    *Shemesh, E., Lurie, S., Stuber, M. L., Emre, S., Patel, Y.,
    Vohra, P., et al. (2000). A pilot study of posttraumatic
    stress and nonadherence in pediatric liver transplant
    recipients. Pediatrics, 105, E29.

    *Stallard, P., Velleman, R., & Baldwin, S. (1998). Prospec-
    tive study of post-traumatic stress disorder in children
    involved in road traffic accidents. British Medical Journal,
    317, 1619-1623.

    Stallard, P., Velleman, R., Langsford, J., & Baldwin, S.
    (2001). Coping and psychological distress in children
    involved in road traffic accidents. British Journal of
    Clinical Psychology, 40, 197-208.

    *Stoddard, F. J., Norman, D. K., Murphy, J. M., & Beardslee,
    W. R. (1989). Psychiatric outcome of burned children
    and adolescents. Journal of the American Academy of
    Child and Adolescent Psychiatry, 28, 589-595.

    Stuber, M. L., Christakis, D. A., Houskamp, B., & Kazak, A.
    E. (1996). Posttrauma symptoms in childhood leukemia
    survivors and their parents. Psychosomatics, 37, 254-261.

    *Stuber, M. L., Nader, K. O., Houskamp, B. M., & Pynoos,
    R. S. (1996). Appraisal of life threat and acute trauma
    responses in pediatric bone marrow transplant patients.
    Journal of Traumatic Stress, 9, 673-686.

    Stuber, M. L., Shemesh, E., & Saxe, G. N. (2003).
    Posttraumatic stress responses in children with life-
    threatening illnesses. Child and Adolescent Psychiatric
    Clinics of North America, 12, 195-209.

    Transplant DataSource. Richmond, VA: United Network for
    Organ Sharing; 2001. Retrieved from www.unos.org.

    Vernberg, E. M., Silverman, W. K., La Greca, A. M., &
    Prinstein, M. J. (1996). Prediction of posttraumatic stress
    symptoms in children after hurricane Andrew. Journal of
    Abnormal Psychology, 105, 237-248.

    *Walker, A. M., Harris, G., Baker, A., Kelly, D., & Houghton,
    J. (1999). Post-traumatic stress responses following liver
    transplantation in older children. Journal of Child
    Psychology and Psychiatry, 40, 363-374.

    Woodruff, T. J., Axelrad, D. A., Kyle, A. D., Nweke, O., Miller,
    G. G., & Hurley, B. J. (2004). Trends in environmentally
    related childhood illnesses. Pediatrics, 113, 1133-1140.

    *Zink, K. A., & McCain, G. C. (2003). Post-traumatic stress
    disorder in children and adolescents with motor vehicle-
    related injuries. Journal for Specialists in Pediatric Nursing,
    8, 99-106.

    Th
    is

    d
    oc

    um
    en

    t i
    s c

    op
    yr

    ig
    ht

    ed
    b

    y
    th

    e A
    m

    er
    ic

    an
    P

    sy
    ch

    ol
    og

    ic
    al

    A
    ss

    oc
    ia

    tio
    n

    or
    o

    ne
    o

    f i
    ts

    a
    lli

    ed
    p

    ub
    lis

    he
    rs

    .
    Th

    is
    a

    rti
    cl

    e
    is

    in
    te

    nd
    ed

    so
    le

    ly
    fo

    r t
    he

    p
    er

    so
    na

    l u
    se

    o
    f t

    he
    in

    di
    vi

    du
    al

    u
    se

    r a
    nd

    is
    n

    ot
    to

    b
    e

    di
    ss

    em
    in

    at
    ed

    b
    ro

    ad
    ly

    .

    Early predictors of chronic post-traumatic stress
    disorder in assault survivors

    BIRGIT KLEIM 1, ANKE EHLERS 1* AND EDWARD GLUCKSMAN 2

    1 Institute of Psychiatry, Department of Psychology, King’s College London, London, UK ; 2 Accident and
    Emergency Department, King’s College Hospital London, London, UK

    ABSTRACT

    Background. Some studies suggest that early psychological treatment is effective in preventing
    chronic post-traumatic stress disorder (PTSD), but it is as yet unclear how best to identify trauma
    survivors who need such intervention. This prospective longitudinal study investigated the prog-
    nostic validity of acute stress disorder (ASD), of variables derived from a meta-analysis of risk
    factors for PTSD, and of candidate cognitive and biological variables in predicting chronic PTSD
    following assault.

    Method. Assault survivors who had been treated for their injuries at a metropolitan Accident and
    Emergency (A&E) Department were assessed with structured clinical interviews to establish diag-
    noses of ASD at 2 weeks (n=222) and PTSD at 6 months (n=205) after the assault. Candidate
    predictors were assessed at 2 weeks.

    Results. Most predictors significantly predicted PTSD status at follow-up. Multivariate logistic
    regressions showed that a set of four theory-derived cognitive variables predicted PTSD best
    (Nagelkerke R2=0.50), followed by the variables from the meta-analysis (Nagelkerke R2=0.37)
    and ASD (Nagelkerke R2=0.25). When all predictors were considered simultaneously, mental
    defeat, rumination and prior problems with anxiety or depression were chosen as the best combi-
    nation of predictors (Nagelkerke R2=0.47).

    Conclusion. Questionnaires measuring mental defeat, rumination and pre-trauma psychological
    problems may help to identify assault survivors at risk of chronic PTSD.

    INTRODUCTION

    After experiencing a violent traumatic event,
    such as an assault or terrorist attack, most
    people show some symptoms of distress, but
    only a minority develop persistent symptoms of
    sufficient severity to warrant a diagnosis of post-
    traumatic stress disorder (PTSD) (Galea et al.
    2002; Rubin et al. 2005; Shalev & Freedman,
    2005). Most people recover from trauma with-
    out formal intervention (Kessler et al. 1995),
    and there is evidence that brief early inter-
    ventions given to all trauma survivors do not

    prevent chronic PTSD (for reviews, see
    NCCMH, 2005; Rose et al. 2002). However,
    early trauma-focused cognitive-behavioural
    treatment of trauma survivors with high levels
    of PTSD symptoms is effective (for recent re-
    views, see NCCMH, 2005; Bisson & Cohen,
    2006), and this treatment may be even more
    effective when delivered within the first weeks
    post-trauma than at later time points (Shalev
    et al. 2006). This has led to recommendations
    that early trauma-focused cognitive-behavioural
    treatment should be offered to trauma survivors
    with severe symptoms, and that screening should
    be considered for populations at risk of chronic
    PTSD (e.g. NCCMH, 2005).

    This raises the question of how best to
    identify trauma survivors who are at risk of

    * Address for correspondence: Professor Anke Ehlers,
    Department of Psychology (PO77), Institute of Psychiatry, De
    Crespigny Park, London SE5 8AF, UK.
    (Email : anke.ehlers@iop.kcl.ac.uk)

    Psychological Medicine, 2007, 37,

    1457

    –1467. f 2007 Cambridge University Press
    doi:10.1017/S0033291707001006 First published online 22 June 2007 Printed in the United Kingdom

    1457

    developing chronic PTSD and need inter-
    vention. One of the motivations for introducing
    the diagnosis of acute stress disorder (ASD) into
    DSM-IV (APA, 1994) was to help to identify,
    within the first weeks post-trauma, those in-
    dividuals who are likely to go on to develop
    PTSD (Marshall et al. 1999). A range of em-
    pirical studies found indeed that the majority of
    trauma survivors with ASD go on to develop
    PTSD (e.g. Harvey & Bryant, 1998; Brewin et al.
    1999). However, several authors have recently
    questioned the predictive validity of the ASD
    diagnosis for subsequent PTSD, and have par-
    ticularly challenged its sensitivity (McNally et al.
    2003; Creamer et al. 2004). Studies with differ-
    ent trauma populations found that on average
    only 46% of those who developed PTSD had
    suffered from ASD in the initial month after
    trauma exposure (McNally et al. 2003) ; pro-
    portions ranged from 10% (severely injured ac-
    cident survivors; Schnyder et al. 2001) to 72%
    (survivors of mild traumatic brain injury;
    Harvey & Bryant, 2000). The low sensitivity
    poses a problem for clinical purposes as it may
    lead to a failure to identify individuals in need of
    treatment.

    A review by Bryant (2003) concluded that
    psychological or biological reactions in the
    acute trauma phase may provide more accurate
    means of predicting PTSD than symptom-based
    measures such as the ASD diagnosis, but this
    hypothesis remains to be tested. The present
    paper explored the question of whether risk
    factors identified in previous research may help
    to improve the early identification of trauma
    survivors who are likely to develop PTSD.

    The first set of predictors under consideration
    was based on a meta-analysis of risk factors for
    PTSD (Ozer et al. 2003). The meta-analysis
    identified seven variables as the best empirically
    established predictors of PTSD; most of these
    had also emerged as risk factors in an earlier
    meta-analysis (Brewin et al. 2000): peritrau-
    matic dissociation (weighted effect size : r=
    0.35), perceived life threat during trauma, peri-
    traumatic emotional responses (both r’s=0.26),
    history of trauma, history of psychological prob-
    lems prior to the trauma, family history of psy-
    chopathology (all r’s=0.17), and post-trauma
    social support (r=x0.28). However, Ozer et al.
    (2003) used the term ‘predictor’ in a statisti-
    cal sense and included many cross-sectional

    studies, some of which were conducted many
    years the trauma. It remains to be tested
    whether the risk factors identified in their meta-
    analysis consistently predict chronic PTSD
    when evaluated in a prospective longitudinal
    design.

    The second set of candidate predictors was
    derived from a cognitive model of PTSD (Ehlers
    & Clark, 2000). This model emphasizes factors
    that maintain PTSD in order to explain why
    some people develop persistent symptoms
    whereas others recover on their own. A series of
    prospective longitudinal studies showed that the
    cognitive factors specified in the model predict
    chronic PTSD at 6 months, 1 year and 3 years
    (Ehlers et al. 1998b, 2003; Dunmore et al. 2001;
    Mayou et al. 2002; Murray et al. 2002; Halligan
    et al. 2003; Michael et al. 2005). The presumed
    maintaining factors also predicted PTSD
    severity at follow-up over and above what
    could be predicted from initial PTSD symptom
    severities. These include the candidate pre-
    dictors chosen for the present study (with cor-
    relations with PTSD severity at 6 months to 1
    year) : mental defeat (Dunmore et al. 2001;
    r=0.64), nowness of trauma memories (Michael
    et al. 2005; r=0.58), negative appraisals of the
    self, including negative interpretation of PTSD
    symptoms (Ehlers et al. 1998b ; Dunmore et al.
    2001; Halligan et al. 2003; r’s=0.43–0.62), and
    rumination about the trauma (Ehlers et al.
    1998b ; r=0.48).

    Finally, the study included resting heart
    rate as an easy-to-measure potential biological
    predictor that could be used in screening pro-
    grammes. A recent review found that eight out
    of 10 studies showed that elevated heart rate in
    the acute trauma phase (measured at varying
    time points between admission to the emergency
    department and 1 month afterwards) is associ-
    ated with later PTSD. Elevated heart rates may
    be a marker of a generalized fear response in
    those who are at risk of PTSD, and may reflect
    the strength of the noradrenergic response to the
    trauma (Bryant, 2006). However, there was
    great variability in heart rate levels and the
    strength of association with subsequent PTSD
    between studies (Bryant, 2006). Bryant et al.
    (2000) found that a diagnosis of ASD or a rest-
    ing heart rate of >90 beats/min (measured
    within 20 days post-trauma) predicted PTSD
    with high sensitivity (88%) and specificity

    1458 B. Kleim et al.

    (85%). Heart rate accounted for 4% of the
    variance of PTSD severity at follow-up.

    The purpose of the present prospective
    longitudinal study was to compare the predic-
    tive power of the ASD diagnosis, the set of seven
    best predictors from Ozer et al.’s (2003) meta-
    analysis, the set of four theory-derived cognitive
    variables, and basal heart rate in the prediction
    of PTSD at 6 months. The 13 predictors were
    assessed at 2 weeks after injury in an assault. We
    investigated which constellation of variables
    would be most predictive of PTSD at 6 months.

    METHOD

    Sample characteristics

    Participants had been injured in an assault
    and were recruited from consecutive attendees
    of King’s College Hospital’s Accident and
    Emergency (A&E) Department, London, UK.
    A total of 1063 assault survivors who attended
    the A&E department during the recruitment
    period (July 2003 to December 2004) were con-
    tacted, of whom 389 did not fulfil inclusion cri-
    teria and 197 declined to take part. Another 255
    were initially interested, but failed to schedule or
    attend the research session within the designated
    time period. The remaining 222 assault sur-
    vivors participated in a diagnostic session at 2
    weeks, and 205 of these (92%) were interviewed
    again at 6 months. Exclusion criteria were cur-
    rent psychosis, assault occurring in the context
    of ongoing domestic violence, and inability to
    remember the assault (e.g. due to head injury).
    Assaults were mainly physical (99%), and 1%
    were sexual assaults. Demographic character-
    istics are listed in Table 1. The study sample was
    compared on demographic and assault charac-
    teristics with a random sample of identical size,
    drawn from 2785 assault survivors admitted to
    the same A&E department during a period of 1
    year. There were no significant differences in
    age, sex or seriousness of injuries as measured
    by triage category. However, the study sample
    comprised more Caucasian participants com-
    pared to the random A&E sample, x2(df=1)=
    17.88, p<0.001, 58.1% v. 36.9%#.

    Education correlated negatively with PTSD
    at 6 months (r=x0.25, p<0.001) and age cor- related positively (r=0.15, p<0.05). None of the other demographic variables distinguished between participants with and without PTSD at 6 months (all p’s>0.07). With respect to assault
    characteristics, the PTSD group reported less
    alcohol or drug consumption during the assault
    [x2(1, n=204)=11.71, p=0.001], but the groups
    did not differ significantly in other variables
    (e.g. injury severity, assault duration, and
    proximity to victims’ home).

    Table 1. Demographic, clinical and assault
    characteristics for participants who completed
    assessments at both 2 weeks and 6 months
    (n=205)

    Variable

    Sex, n (%)
    Male 140 (68.0)
    Female 65 (32.0)

    Age, mean (S.D.) 35.0 (11.5)
    Ethnicity, n (%)
    Caucasian 119 (58.0)
    Black 67 (32.7)
    Other 19 (9.3)

    Socio-economic statusa, n (%)
    Very low income (£5000 or less) 59 (28.8)
    Low income (£5000–£15000) 56 (27.3)
    Moderate income (£15000–£30000) 43 (21.0)
    High income (over £30000) 35 (17.1)
    Refused information 12 (5.9)

    Marital status, n (%)
    Single 136 (66.3)
    Married 39 (19.0)
    Divorced/separated 23 (11.2)
    Widowed 2 (1.0)
    No information 5 (2.5)

    Education, mean (S.D.) 14.1 (4.7)

    Acute stress disorder at 2 weeks, n (%)
    Fulfilled diagnostic criteria 34 (16.6)

    Post-traumatic stress disorder at
    6 months, n (%)
    Fulfilled diagnostic criteria 49 (23.9)

    Days since assault, mean (S.D.) 17.5 (7.65)
    Number of assailants, n (%)
    1 107 (52)
    2 or more 97 (48)

    Weapon involved, n (%)
    Yes 106 (52)
    No 98 (48)

    Survivor under influence of
    alcohol/drugs, n (%)
    Yes 87 (43)
    No 117 (57)

    S.D., Standard deviation.
    Assault characteristics were missing for one participant.
    a Combined household income.

    # The figure of 36.9% presents an underestimate of the number of
    Caucasian participants. It is based on A&E department codes that
    require the staff to code ethnicity in 80 categories based on country of
    origin. In practice, the category of ‘other’ was often used for non-
    British participants regardless of their ethnic background.

    Early predictors of chronic PTSD 1459

    Measures

    Diagnostic interviews

    ASD and PTSD diagnoses were established with
    standard structured clinical interviews: the
    Acute Stress Disorder Scale (Bryant & Harvey,
    2000) and the Structured Clinical Interview
    for DSM-IV (SCID; First et al. 1996). All in-
    terviews were conducted by B.K. under the
    supervision of A.E. Inter-rater reliability was
    excellent (k=0.97 for ASD, k=0.82 for PTSD;
    based on n=56 interviews, two trained raters).

    Predictors from Ozer et al.’s (2003)
    meta-analysis

    Psychological problems prior to trauma. Par-
    ticipants reported whether or not they had had
    problems with anxiety or depression in the past.
    This self-report measure correlated significantly
    with the SCID assessment of a history of major
    depression (r=0.49, p<0.001).

    Family history of psychological problems. Par-
    ticipants reported whether any of their close
    family members had ever had a problem with
    anxiety or depression.

    Perceived social support following trauma. A
    modified subscale from the Crisis Support Scale
    (CSS; Joseph, 1999) assessed social support
    soon after the assault (a=0.75). Participants
    rated seven different aspects of social support
    (e.g. ‘Whenever you wanted to talk, how often
    was there someone willing to listen?’) on a scale
    from 1 (never) to 7 (always). An original item
    enquiring about personal contact with other
    trauma survivors was omitted because it did not
    seem to be relevant for the majority of assault
    survivors.

    Perceived life threat. Participants rated how
    much they feared for their life during the as-
    sault, on a scale from 0 (not at all) to 4 (very
    strongly). This rating has been shown to predict
    PTSD (Dunmore et al. 2001; Halligan et al.
    2003).

    Peritraumatic emotional response. This was
    measured on a seven-item scale (Halligan et al.
    2003; a=0.89). Participants rated the extent to
    which they had experienced the following
    emotions during the assault : terrified, ashamed,

    helpless, fearful, guilty, horrified, frightened,
    each on a scale from 0 (not at all) to 4 (very
    strongly).

    Peritraumatic dissociation. Dissociation during
    the assault was assessed with the State
    Dissociation Questionnaire (Murray et al. 2002;
    a=0.88), a nine-item scale assessing different
    aspects of dissociation such as derealization,
    depersonalization, detachment, altered time
    sense, emotional numbing, and reduction of
    awareness in surroundings. The scale has been
    shown to have good reliability and predictive
    validity in previous studies (e.g. Halligan et al.
    2002).

    Theory-derived cognitive predictors

    Four more variables were chosen on the basis
    of earlier studies to each represent one of the
    four hypothesized cognitive domains specified
    in Ehlers & Clark’s (2000) model of PTSD.
    The model states that people with PTSD
    experience a sense of current threat that arises
    from two sources : (1) excessively negative ap-
    praisals of the trauma and/or its consequences,
    and (2) certain characteristics of the trauma
    memory that lead to easy cue-driven retrieval
    (i.e. unintentional triggering by matching stim-
    uli) of aspects of the trauma memory. These
    memory characteristics are thought to result
    from (3) the quality of cognitive processing
    during the event. Finally, a range of (4) cogni-
    tive and behavioural strategies (e.g. rumination,
    thought suppression, excessive precautions,
    and ongoing dissociation) that individuals use
    to control the threat and symptoms are thought
    to maintain PTSD.

    Negative appraisals of the self. Negative ap-
    praisals of the self were measured with the
    Negative Thoughts about the Self subscale
    of the Posttraumatic Cognitions Inventory
    (PTCI; 21 items; a=0.93; Foa et al. 1999).
    The scale measures generalized negative
    appraisals of the trauma and its aftermath
    (e.g. ‘My reactions since the event mean that I
    am going crazy’, ‘I have permanently changed
    for the worse ’, ‘I am a weak person’) and has
    been shown to have good reliability, convergent
    validity and to discriminate well between trau-
    matized people with and without PTSD (Foa
    et al. 1999).

    1460 B. Kleim et al.

    ‘Nowness ’ of trauma memories. Trauma mem-
    ories are thought to be disjointed from other
    autobiographical memories, leading to a deficit
    in the awareness that they reflect an experience
    of the self in the past (Ehlers et al. 2004).
    Perceived ‘nowness’ of traumatic memories
    was measured with the corresponding item from
    the Assault Memory Questionnaire (Halligan
    et al. 2003) : ‘When I remember the assault,
    it is like happening again, here and now’.
    Self-reported ‘nowness’ correlates well with in-
    terview assessment (r=0.84; Hackmann et al.
    2004), and the retest reliability is r=0.68
    (Speckens et al. 2006).

    Mental defeat. Mental defeat refers to pro-
    cessing the trauma as a complete loss of per-
    sonal autonomy and has been shown to predict
    PTSD in survivors of assault and torture
    (Dunmore et al. 1999, 2001; Ehlers et al. 2000).
    The Mental Defeat Scale (Dunmore et al. 1999,
    2001; a=0.90) is an 11-item self-report ques-
    tionnaire. Participants rated the extent to
    which statements such as ‘I no longer felt like
    a human being’ or ‘In my mind, I gave up’ ap-
    plied to them at some time during the assault,
    each on a scale from 0 (not at all) to 4 (very
    strongly).

    Rumination. Rumination about the trauma
    and its consequences was measured with the
    eight-item subscale of the Response to
    Intrusions Questionnaire (Clohessy & Ehlers,
    1999; Murray et al. 2002; a=0.84). Participants
    rated how often they did things such as ‘I think
    about why the event happened to me’ or ‘I go
    over and over the assault ’, when memories
    of the assault popped into their mind, each on
    a scale from 0 (never) to 3 (always). The scale
    has been shown to have good reliability and
    predictive validity (Murray et al. 2002; Ehring
    et al. 2006).

    Resting heart rate

    Heart inter-beat intervals were registered with
    an S810 Polar watch (Polar Electro, Vantaa,
    Finland) for a period of 3 min, while partici-
    pants remained seated. Participants were in-
    structed to sit quietly, not to speak and to keep
    their eyes open. Heart rate data were available
    for 177 participants (80%). The Polar device
    has been shown to reliably assess inter-beat

    intervals during rest as well as activity
    (e.g. Laukkanen & Virtanen, 1998). None of
    the participants were taking beta-blockers or
    other medication affecting the cardiovascular
    system.

    Procedure

    The study was approved by the local ethics
    committees, and participants gave written
    consent. Participants completed a research
    session comprising the completion of ques-
    tionnaires assessing the psychological predictor
    variables, heart rate assessment and the
    ASD Scale (Bryant & Harvey, 2000) interview.
    At 6 months, the SCID (First et al. 1996) was
    conducted over the telephone by the same
    interviewer. Participants received £50 as reim-
    bursement for their time.

    Statistical analysis

    SPSS version 13.0 (SPSS Inc., Chicago, IL,
    USA) was used for most analyses ; bootstrap
    analyses were implemented by Stata Statistics
    and Data Analysis Package version 9.0
    (Stata Corporation, College Station, TX, USA).
    Predictors were z-standardized to allow direct
    comparison of odds ratios (ORs). Association
    between candidate predictors and PTSD
    were first investigated with univariate logistic
    regression analyses. Hierarchical logistic re-
    gression analysis tested which of the predictors
    explained PTSD at 6 months over and
    above what could be predicted from the ASD
    diagnosis, by forcing ASD into the equation
    in the first step and the predictor in the
    second step. In addition, two multivariate
    logistic regression analyses tested the predictive
    power of the variable sets from Ozer et al.’s
    (2003) meta-analysis and from the cognitive
    model (including only significant univariate
    predictors). Finally, a logistic regression analy-
    sis of all significant univariate predictors
    identified the combination of predictors that
    best predicted PTSD at 6 months; this model
    determined which variables to add to the
    model based on the likelihood ratio test.
    Reliability of univariate results was established
    by calculating confidence intervals (CIs) for
    ORs of all predictors with bootstrap resampling
    (Efron & Tibshirani, 1986), which involved
    drawing 1000 random subsamples of the total
    sample.

    Early predictors of chronic PTSD 1461

    RESULTS

    ASD and PTSD prevalence

    At 2 weeks, 17% of the participants (n=37/222)
    met diagnostic criteria forASD, and at 6months,
    24% (n=49/205) met diagnostic criteria for
    PTSD.

    Early predictors of chronic PTSD

    Table 2 shows the associations between the
    predictors assessed at 2 weeks and PTSD at

    6 months. Most univariate associations were
    significant. Participants with ASD were at in-
    creased risk for PTSD at 6 months compared
    to those without ASD (non-transformed OR
    11.7, w=0.46). Table 2 shows that although the
    majority of participants with ASD went on to
    develop chronic PTSD (68%), ASD had low
    sensitivity (47%) in predicting PTSD.

    Most of the predictors derived from Ozer
    et al.’s (2003) meta-analysis were significantly
    associated with a greater PTSD risk (prior

    Table 2. Associations of predictors at 2 weeks with PTSD diagnosis at 6 months

    Predictor

    Diagnosis at 6 months

    Univariate logistic regression

    Multiple logistic regression Sign predictor
    after controlling

    for ASDPTSD No PTSD ORa 95% CI

    Boot-
    strapped

    CI ORa 95% CI

    Acute stress disorder R2=0.25 (95% CI 0.15–0.35), x2=36.89***
    ASD diagnosis

    Yes 23 11 2.50*** 1.84–3.41 1.13–2.96 2.50*** 1.84–3.41 N.A.
    No 26 145

    Risk factors from the

    Ozer et al. (2003)
    meta-analysis

    R2=0.37 (95% CI 0.24–0.47), x2=48.22***

    Prior trauma
    Yes 46 142 1.47 0.87–2.47 0.28–1.44 Not includedb 0.225
    No 1 46

    Prior psychological
    problems
    Yes 31 45 2.10*** 1.50–2.95 0.84–3.18 2.06** 1.35–3.16 0.001
    No 16 107

    Family history of
    psychological problems
    Yes 14 37 1.15 0.83–1.58 0.75–2.34 Not includedb 0.623
    No 32 115

    Perceived life threat,
    mean (S.D.)

    2.25 (1.59) 1.05 (1.46) 2.12*** 1.52–2.96 0.78–2.27 1.22 0.75–1.98 0.006

    Peritraumatic emotional
    response, mean (S.D.)

    2.61 (0.96) 1.66 (1.04) 2.77*** 1.85–4.14 0.83–3.06 2.43** 1.32–4.50 0.001

    Perceived social support,
    mean (S.D.)

    3.35 (1.34) 3.79 (1.17) 0.70* 0.50–1.00 0.29–1.40 0.64* 0.42–3.16 0.112

    Peritraumatic dissociation,
    mean (S.D.)

    2.24 (0.94) 1.31 (1.09) 2.35*** 1.65–3.52 1.04–3.04 1.42 0.86–2.35 0.011

    Theory-derived cognitive

    measures

    R2=0.50 (95% CI 0.38–0.59), x2=68.91***

    Mental defeat, mean (S.D.) 2.15 (1.09) 0.89 (0.80) 3.76*** 2.51–5.65 1.29–3.51 1.74 0.97–3.11 0.000
    Nowness of trauma
    memories, mean (S.D.)

    2.21 (1.53) 0.73 (1.10) 2.89*** 1.99–4.19 1.18–4.81 1.67* 1.00–2.79 0.000

    Negative appraisals of the
    self, mean (S.D.)

    3.47 (1.20) 2.19 (0.96) 3.18*** 2.09–4.82 1.36–4.75 1.99* 1.13–3.50 0.000

    Rumination, mean (S.D.) 1.79 (0.60) 1.00 (0.58) 4.19*** 2.52–7.01 1.16–3.26 1.92* 1.04–3.56 0.000

    Biological measure R2=0.04 (95% CI 0.00–0.11), x2=4.90*
    Resting heart rate,
    mean (S.D.)

    71.80 (12.35) 67.10 (10.80) 1.50* 1.04–2.15 0.77–2.99 1.50* 1.04–2.15 0.049

    PTSD, Post-traumatic stress disorder ; ASD, acute stress disorder ; S.D., standard deviation; N.A., not applicable ; CI, confidence interval.
    a Predictors are z-standardized to make odds ratios comparable ; b as non-significant in univariate analysis.
    Questionnaire ranges : mental defeat 0–4, nowness of trauma memories 0–4, negative appraisals about the self 1–7, rumination 0–4,

    perceived life threat 0–4, perceived support 1–7, peritraumatic dissociation 0–4.
    *** p<0.001, ** p<0.01, * p<0.05.

    1462 B. Kleim et al.

    psychological problems, less post-trauma social
    support, greater perceived threat to life, peri-
    traumatic emotional responses and dissociation
    during the assault). Associations with prior
    trauma (non-transformed OR 4.54, 95% CI
    0.58–35.45, w=0.11) and family history of
    psychological problems (non-transformed OR
    1.36, 95% CI 0.66–2.82, w=0.06) did not
    reach significance. All theory-derived cognitive
    variables (mental defeat, nowness of memories,
    negative appraisals of the self, and rumination
    about the trauma) and resting heart rate pre-
    dicted PTSD. Bootstrap analyses for 1000 sub-
    samples showed that ORs and CIs replicated
    well. Table 2 also shows which of the variables
    predicted PTSD at 6 months over and above
    what could be predicted from an ASD diagnosis
    at 2 weeks. These were the four theory-derived
    cognitive variables, prior psychological prob-
    lems, perceived life threat, peritraumatic
    emotional response and dissociation, and rest-
    ing heart rate.

    Next, we compared the predictive power
    of the sets of variables under investigation
    (Table 2). The theory-derived cognitive vari-
    ables explained 50% of the variance (sensitivity
    0.60, specificity 0.95, 88% correct classifi-
    cations). CIs indicated that the strength of the
    prediction was significantly larger than the 25%
    explained by an ASD diagnosis (sensitivity 0.47,
    specificity 0.93, 82% correct classifications),
    and the 4% explained by resting heart rate at 2
    weeks (sensitivity 0.00, specificity 1.00). The es-
    tablished predictors identified in Ozer et al.’s
    (2003) meta-analysis explained 37% of the
    variance (sensitivity 0.44, specificity 0.95, 85%
    correct classifications), which was not signifi-
    cantly different from the predictive power of the
    ASD diagnosis.

    When all significant predictors were con-
    sidered in an overall multiple logistic regression
    analysis, mental defeat (OR 2.07, p=0.014,
    95% CI 1.16–3.70), rumination about the trau-
    ma (OR 2.99, p=0.002, 95% CI 1.50–5.96) and
    prior psychological problems (OR 1.95,
    p=0.014, 95% CI 1.14–3.31) were chosen as the
    best combination of predictors by the forward
    likelihood ratio test (x2=47.37, p=0.000).
    Together, the three variables explained 47% of
    the variance (sensitivity 0.57, specificity 0.96)
    and classified 88% of the cases correctly into
    PTSD and non-PTSD status. The Hosmer and

    Lemeshow goodness-of-fit test showed that the
    model fit the data well (x2=9.86, p=0.275). Not
    selected were ASD (after last step p=0.184),
    perceived threat to life (p=0.452), peritraumatic
    dissociation (p=0.941), peritraumatic emotion-
    al response (p=0.695), nowness of trauma
    memories (p=0.138), negative appraisals about
    the self (p=0.589) and resting heart rate
    (p=0.828).

    DISCUSSION

    This prospective longitudinal study found that
    17% of 222 injured assault survivors met diag-
    nostic criteria for ASD at 2 weeks after the
    event, and at 6 months, 24% had PTSD. These
    prevalences are similar to those reported in
    other studies of assault survivors (e.g. Harvey
    & Bryant, 1998; Brewin et al. 1999). While the
    majority of participants with ASD went on to
    develop chronic PTSD (68%), ASD had low
    sensitivity (47%) in predicting PTSD, again in
    accord with previous studies (Brewin et al. 1999;
    Creamer et al. 2004; Elklit & Brink, 2004; see
    McNally et al. 2003 for a review). One of the
    reasons for the low sensitivity of ASD may be
    that not all assault survivors at risk for PTSD
    may have had the three dissociative symptoms
    required for a diagnosis. Overall, the usefulness
    of ASD as a criterion for identifying people at
    risk of PTSD was limited.

    This raises the question of whether there are
    other ways of identifying people at risk for
    chronic PTSD (e.g. Shalev et al. 1997; Brewin
    et al. 2002). The present study tested three
    groups of candidate predictors of PTSD ident-
    ified in previous research, namely the seven best
    established predictors of PTSD according to the
    meta-analysis of Ozer et al. (2003), four pre-
    dictors derived from a cognitive model of PTSD
    (Ehlers & Clark, 2000), and resting heart rate as
    a candidate biological predictor. In line with
    previous findings, nearly all of these variables
    predicted PTSD at 6months (Ehlers et al. 1998b ;
    Brewin et al. 2000; Bryant et al. 2000; Dunmore
    et al. 2001; Murray et al. 2002; Halligan et al.
    2003; Ozer et al. 2003). The results extend pre-
    vious findings in several ways. (1) They demon-
    strated that the predictors not only are
    associated with concurrent PTSD but also pre-
    dict chronic PTSD prospectively ; this strength-
    ens Ozer et al.’s (2003) findings, which were

    Early predictors of chronic PTSD 1463

    based largely on cross-sectional studies. (2) The
    variables were predictive even when assessed
    2 weeks after the trauma, which extends the
    results of previous prospective longitudinal
    studies where the initial assessment took place
    later (e.g. Dunmore et al. 2001). (3) The study
    allowed a direct comparison of the strength
    of the prediction of different sets of variables
    suggested as relevant in the literature. (4)
    Recruitment from an A&E department ensured
    a sample that was representative in terms of the
    severity of assaults and most other demographic
    characteristics.

    Overall, the results replicated well those of the
    Ozer et al. (2003) meta-analysis. As in the meta-
    analysis, peritraumatic variables (peritraumatic
    dissociation and emotions, perceived life threat)
    and social support tended to be stronger pre-
    dictors than pre-trauma characteristics (see
    also Brewin et al. 2000). The finding that the
    relationships between prior trauma and a family
    history of psychological problems and PTSD
    did not reach significance in this study should
    not be overinterpreted. Given the modest PTSD
    rate in the sample, our study had low power for
    detecting very small effect sizes. Restrictions in
    range may have played a role as the majority of
    the participants reported prior trauma.
    However, it is also possible that the type of
    trauma or timing of assessment plays a role in
    the strength of the relationship of these vari-
    ables with PTSD, as other studies of single-
    event trauma have also failed to establish a
    relationship between family history and PTSD
    (Blanchard & Hickling, 2004) and as Ozer et al.
    (2003) identified three previous studies of recent
    trauma survivors (f4 months), all of which
    found no relationship between trauma history
    and PTSD (r’s=0.00–0.03).

    The set of predictors from the Ozer et al.
    (2003) meta-analysis did not significantly pre-
    dict chronic PTSD better than the ASD diag-
    nosis. A possible explanation may be that these
    variables mainly predict the onset of symptoms,
    but are less useful for the prediction of long-
    term outcome as maintaining factors are not
    included (e.g. Ehlers & Steil, 1995). By contrast,
    the theory-derived cognitive factors each pre-
    dicted PTSD at 6 months over and above what
    can be predicted from an ASD diagnosis at
    2 weeks, and together explained more variance
    than ASD. This finding may reflect the fact

    that PTSD symptoms show substantial natural
    recovery in the first months after trauma
    (e.g. Rothbaum et al. 1992; Kessler et al. 1995),
    and factors that impede recovery and maintain
    PTSD symptoms may therefore be of particular
    interest in predicting chronic PTSD (e.g. Ehlers
    & Steil, 1995; Ehlers & Clark, 2000).

    The logistic regression analysis that con-
    sidered all candidate predictors simultaneously
    identified a combination of two of such theory-
    derived maintaining variables (mental defeat,
    rumination) and a vulnerability variable (psy-
    chological problems prior to the assault) as
    the best predictor set. This suggests that the
    combination of vulnerability and maintaining
    factors may provide the best prediction of
    chronic PTSD. The combination of these three
    predictors predicted diagnostic status at 6
    months correctly in 88% of the cases. Together,
    the three variables explained almost half of the
    variance in PTSD diagnosis at 6 months, com-
    pared to 25% of explained variance by the ASD
    diagnosis.

    In line with previous research, resting heart
    rate at 2 weeks predicted PTSD at 6 months, but
    only explained a small proportion of the vari-
    ance. Bryant (2006) proposed multiple path-
    ways to PTSD development, some of which are
    characterized by elevated and others by lowered
    heart rate levels (alongside dissociative re-
    sponses). Such differences in response pattern
    would be consistent with the present results, but
    limit the usefulness of basal heart rate as a way
    of early screening for risk of chronic PTSD.
    Heart rate may be more predictive if it is as-
    sessed sooner after the trauma, although the
    currently available data show great variation for
    the predictive power of such early assessments
    (Bryant, 2006). It is also possible that the parti-
    cipants’ heart rate may have been influenced by
    memories triggered by the questionnaires that
    were part of the research session, although such
    responses would have been expected to increase
    rather than decrease the relationship between
    heart rate and PTSD.

    The study had strengths and limitations.
    Among the strength are the prospective longi-
    tudinal design, the relatively large number of
    participants, the excellent follow-up rate, the
    use of reliable structured interviews to establish
    diagnoses, the replication of the results in
    bootstrapping analyses, and the early initial

    1464 B. Kleim et al.

    assessment. Nevertheless, further research will
    need to establish how well the present results
    generalize to other populations with different
    PTSD rates and other forms of trauma before
    the results are applied to clinical practice. The
    sample showed a moderate PTSD rate of 24%
    at follow-up, and had low power to detect very
    small effect sizes. Thirteen potential predictors
    were considered, which raises the possibility of
    false positive associations. However, all of the
    predictors had been shown to be associated with
    PTSD risk in previous studies, and the present
    sample replicated earlier results well and showed
    good replication in bootstrapping analyses.
    Although our analyses indicated that the sample
    was fairly representative of assault survivors
    attending the A&E department, it comprised
    relatively fewer people of ethnic minorities
    compared to the population of assault survivors
    treated at this hospital (nevertheless, the 42%
    exceeded the 24% ethnic minorities living in
    London according to the 2001 Census). The
    concept of mental defeat, one of the strongest
    predictors in this study, was developed with
    survivors of interpersonal violence such as
    assault and torture (e.g. Dunmore et al. 1999,
    2001; Ehlers et al. 1998a, 2000), and other pre-
    dictors may be more relevant for survivors of
    other traumas such as accidents or natural
    disaster. A further possible limitation was that
    PTSD diagnoses at 6 months were based on
    a diagnostic interview conducted over the tele-
    phone, rather than a face-to-face diagnostic
    interview. It may be argued that telephone in-
    terviews make participants somewhat less forth-
    coming in providing information about their
    symptoms than face-to-face interviews. How-
    ever, the interviewer had already established a
    relationship with the participants at the first as-
    sessment, which makes it unlikely that partici-
    pants withheld information from her later.
    Finally, personal and family history of problems
    with anxiety or depression was assessed with
    simple self-report questions, and more sophisti-
    cated assessments may have given different re-
    sults.

    In conclusion, this study found that PTSD
    at 6 months after injury in an assault can be
    predicted from early assessment at 2 weeks.
    A set of four theory-derived cognitive variables
    predicted PTSD better than the ASD diagnosis.
    Logistic regression analysis suggested that

    the combination of mental defeat, rumination
    and a history of problems with anxiety or
    depression predicted PTSD best. These concepts
    can be measured with only 20 self-report
    questions. However, it remains to be tested how
    the three selected scales perform on their
    own, as they were selected from a larger group
    of 11 significant predictors in this study. If
    replicated, our results suggest that assault
    survivors are at heightened PTSD risk if they
    report mental defeat at the time of the event,
    if they ruminate about the trauma, and if they
    report prior problems with depression or anxi-
    ety. Early assessment of these predictors
    appears straightforward and may be useful
    for screening programmes in general practice
    or communities affected by trauma. They
    may help to identify those trauma survivors
    who are most likely to develop PTSD and
    who would benefit from early intervention.
    Putting the experience of mental defeat in
    perspective and reducing rumination appear
    to be promising targets for such interventions.

    ACKNOWLEDGEMENTS

    This study was funded by a grant from the
    Psychiatry Research Trust and a Wellcome
    Trust Principal Research Fellowship to Anke
    Ehlers. We thank Thomas Ehring, Silke Frank,
    Inga Boellinghaus, Emma Briddon, Anke
    Weidmann, Ines Sengstock, Johanna Hissbach,
    Jennifer Baumeister, Stephanie Spengler and the
    staff of King’s College Accident and Emergency
    Department for their help.

    DECLARATION OF INTEREST

    None.

    REFERENCES

    APA (1994). Diagnostic and Statistical Manual of Mental Disorders
    (4th edn). American Psychiatric Association: Washington, DC.

    Bisson, J. I. & Cohen, J. A. (2006). Disseminating early interventions
    following trauma. Journal of Traumatic Stress 19, 583–595.

    Blanchard, E. B. & Hickling, E. J. (2004). After the Crash:
    Psychological Assessment and Treatment of Survivors of Motor
    Vehicle Accidents (2nd edn). American Psychiatric Association:
    Washington, DC.

    Brewin, C. R., Andrews, B., Rose, S. & Kirk, M. (1999). Acute stress
    disorder and posttraumatic stress disorder in victims of violent
    crime. American Journal of Psychiatry 156, 360–366.

    Brewin, C. R., Andrews, B. & Valentine, J. D. (2000). Meta-analysis
    of risk factors for posttraumatic stress disorder in trauma-
    exposed adults. Journal of Consulting and Clinical Psychology 68,
    748–766.

    Early predictors of chronic PTSD 1465

    Brewin, C. R., Rose, S., Andrews, B., Green, J., Tata, P., McEvedy,

    C., Turner, S. & Foa, E. B. (2002). Brief screening instrument for
    post-traumatic stress disorder. British Journal of Psychiatry 181,
    158–162.

    Bryant, R. A. (2003). Early predictors of posttraumatic stress dis-
    order. Biological Psychiatry 53, 789–795.

    Bryant, R. A. (2006). Longitudinal psychophysiological studies of
    heart rate: mediating effects and implications for treatment.
    Annals of the New York Academy of Sciences 1071, 19–26.

    Bryant, R. A. & Harvey, A. G. (2000). Acute Stress Disorder: A
    Handbook of Theory, Assessment, and Treatment. American
    Psychological Association: Washington, DC.

    Bryant, R. A., Harvey, A. G., Guthrie, R. M. &Moulds, M. L. (2000).
    A prospective study of psychophysiological arousal, acute stress
    disorder, and posttraumatic stress disorder. Journal of Abnormal
    Psychology 109, 341–344.

    Clohessy, S. & Ehlers, A. (1999). PTSD symptoms, response to
    intrusive memories and coping in ambulance service workers.
    British Journal of Clinical Psychology 38, 251–265.

    Creamer, M., O’Donnell, M. L. & Pattison, P. (2004). The relation-
    ship between acute stress disorder and posttraumatic stress dis-
    order in severely injured trauma survivors. Behaviour Research and
    Therapy 43, 315–328.

    Dunmore, E., Clark, D. M. & Ehlers, A. (1999). Cognitive factors
    involved in the onset and maintenance of posttraumatic stress
    disorder (PTSD) after physical or sexual assault. Behaviour
    Research and Therapy 37, 809–829.

    Dunmore, E., Clark, D. M. & Ehlers, A. (2001). A prospective inves-
    tigation of the role of cognitive factors in persistent posttraumatic
    stress disorder (PTSD) after physical or sexual assault. Behaviour
    Research and Therapy 39, 1063–1084.

    Efron, B. & Tibshirani, R. (1986). Bootstrap methods for standard
    errors, confidence intervals and other measures of statistical
    accuracy. Statistical Science 1, 54–77.

    Ehlers, A. & Clark, D. M. (2000). A cognitive model of posttraumatic
    stress disorder. Behaviour Research and Therapy 38, 319–345.

    Ehlers, A., Clark, D. M., Dunmore, E., Jaycox, L., Meadows, E.

    & Foa, E. B. (1998a). Predicting response to exposure treatment in
    PTSD: the role of mental defeat and alienation. Journal of
    Traumatic Stress 11, 457–471.

    Ehlers, A., Hackmann, A. & Michael, T. (2004). Intrusive re-
    experiencing in post-traumatic stress disorder : phenomenology,
    theory, and therapy. Memory 12, 403–415.

    Ehlers, A., Maercker, A. & Boos, A. (2000). Posttraumatic stress
    disorder following political imprisonment: the role of mental de-
    feat, alienation, and perceived permanent change. Journal of
    Abnormal Psychology 109, 45–55.

    Ehlers, A., Mayou, R. A. & Bryant, B. (1998b). Psychological
    predictors of chronic posttraumatic stress disorder after
    motor vehicle accidents. Journal of Abnormal Psychology 107,
    508–519.

    Ehlers, A., Mayou, R. A. & Bryant, B. (2003). Cognitive predictors of
    posttraumatic stress disorder in children: results of a prospective
    longitudinal study. Behaviour Research and Therapy 41, 1–10.

    Ehlers, A. & Steil, R. (1995). Maintenance of intrusive memories in
    posttraumatic stress disorder: a cognitive approach. Behavioural
    and Cognitive Psychotherapy 23, 217–249.

    Ehring, T., Ehlers, A. & Glucksman, E. (2006). Contribution of cog-
    nitive factors to the prediction of post-traumatic stress disorder,
    phobia and depression after motor vehicle accidents. Behaviour
    Research and Therapy 44, 1699–1716.

    Elklit, A. & Brink, O. (2004). Acute stress disorder as a predictor of
    post-traumatic stress disorder in physical assault victims. Journal
    of Interpersonal Violence 19, 709–726.

    First, M. B., Spitzer, R. L., Gibbon, M. & Williams, J. B. W. (1996).
    Structured Clinical Interview for DSM-IV Axis I Disorders.
    American Psychiatric Press : Washington, DC.

    Foa, E. B., Ehlers, A., Clark, D. M., Tolin, D. F. & Orsillo, S. M.

    (1999). The Posttraumatic Cognitions Inventory (PTCI) :
    development and validation. Psychological Assessment 11, 303–
    314.

    Galea, S., Ahern, J., Resnick, H., Kilpatrick, D., Bucuvalas, M., Gold,

    J. & Vlahov, D. (2002). Psychological sequelae of the September 11
    terrorist attacks in New York City. New England Journal of
    Medicine 346, 982–987.

    Hackmann, A., Ehlers, A., Speckens, A. & Clark, D. M. (2004).
    Characteristics and content of intrusive memories in PTSD and
    their changes with treatment. Journal of Traumatic Stress 17,
    231–240.

    Halligan, S. L., Clark, D. M. & Ehlers, A. (2002). Cognitive pro-
    cessing, memory, and the development of PTSD symptoms: two
    experimental analogue studies. Journal of Behavior Therapy and
    Experimental Psychiatry 33, 73–89.

    Halligan, S. L., Michael, T., Clark, D. M. & Ehlers, A. (2003).
    Posttraumatic stress disorder following assault : the role of cogni-
    tive processing, trauma memory, and appraisals. Journal of
    Consulting and Clinical Psychology 71, 419–431.

    Harvey, A. G. & Bryant, R. A. (1998). The relationship between acute
    stress disorder and posttraumatic stress disorder: a prospective
    evaluation of motor vehicle accident survivors. Journal of
    Consulting and Clinical Psychology 66, 507–512.

    Harvey, A. G. & Bryant, R. A. (2000). A two-year pro-
    spective evaluation of the relationship between acute stress
    disorder and posttraumatic stress disorder following mild
    traumatic brain injury. American Journal of Psychiatry 157, 626–
    628.

    Joseph, S. (1999). Social support and mental health following trau-
    ma. In Post-traumatic Stress Disorder: Concepts and Therapy
    (ed. W. Yule), pp. 71–91. Wiley : Chichester.

    Kessler, R. C., Sonnega, A., Bromet, E., Hughes, M. & Nelson, C. B.

    (1995). Posttraumatic stress disorder in the National Comorbidity
    Survey. Archives of General Psychiatry 52, 1048–1060.

    Laukkanen, R. M. T. & Virtanen, P. (1998). Heart rate monitors :
    state of the art. Journal of Sports Sciences 16, 3–7.

    Marshall, R. D., Spitzer, R. & Liebowitz, M. R. (1999). Review and
    critique of the new DSM-IV diagnosis of acute stress disorder.
    American Journal of Psychiatry 156, 1677–1685.

    Mayou, R. A., Ehlers, A. & Bryant, B. (2002). Posttraumatic stress
    disorder after motor vehicle accidents : 3-year follow-up of a
    prospective longitudinal study. Behaviour Research and Therapy
    40, 665–675.

    McNally, R. J., Bryant, R. A. & Ehlers, A. (2003). Does early
    psychological intervention promote recovery from posttraumatic
    stress? Psychological Science in the Public Interest 4, 45–79.

    Michael, T., Ehlers, A., Halligan, S. & Clark, D. M. (2005).
    Unwanted memories of assault : what intrusion charac-
    teristics predict PTSD? Behaviour Research and Therapy 43, 613–
    628.

    Murray, J., Ehlers, A. & Mayou, R. A. (2002). Dissociation and
    post-traumatic stress disorder : two prospective studies of road
    traffic accident survivors. British Journal of Psychiatry 180, 363–
    368.

    NCCMH (2005). Post-traumatic stress disorder: the management of
    PTSD in adults and children in primary and secondary care.
    National Collaborating Centre for Mental Health. National
    Institute for Clinical Excellence (NICE): London, UK.

    Ozer, E. J., Best, S. R., Lipsey, T. L. & Weiss, D. S. (2003).
    Predictors of posttraumatic stress disorder and symptoms in
    adults : a meta-analysis. Psychological Bulletin 129, 52–73.

    Rose, S., Bisson, J., Churchill, R. & Wesseley, S. (2002). Psycho-
    logical debriefing for preventing posttraumatic stress disorder. The
    Cochrane Database of Systematic Reviews. Issue 2. Article No.:
    CD00560.

    Rothbaum, B. O., Foa, E. B., Riggs, D. S., Murdock, T. &

    Walsh, W. (1992). A prospective examination of posttraumatic
    stress disorder in rape victims. Journal of Traumatic Stress 5,
    455–475.

    Rubin, G. J., Brewin, C. R., Greenberg, N., Simpson, J. &

    Wessely, S. (2005). Psychological and behavioural reactions to
    the bombings in London on 7 July 2005: cross sectional survey of a
    representative sample of Londoners. British Medical Journal 331,
    606.

    1466 B. Kleim et al.

    Schnyder, U., Moergli, H., Klaghofer, R. & Buddeberg, C. (2001).
    Incidence and prediction of posttraumatic stress disorder symp-
    toms in severely injured accident victims. American Journal of
    Psychiatry 158, 594–599.

    Shalev, A. Y. & Freedman, S. (2005). PTSD following terrorist at-
    tacks: a prospective evaluation. American Journal of Psychiatry
    170, 558–564.

    Shalev, A. Y., Freedman, S., Adessky, R. & Watson, P. (2006). Who
    needs care, who wants care, who is helped by early intervention:
    5600 trauma survivors’ results. Abstract no. 159877. In

    Symposium at the 22nd Annual Meeting of the International
    Society for Traumatic Stress Studies, Hollywood, CA, USA.

    Shalev, A. Y., Freedman, S., Peri, T., Brandes, D. & Sahar, T. (1997).
    Predicting PTSD in trauma survivors: prospective evaluation of
    self-report and clinician-administered instruments. British Journal
    of Psychiatry 170, 558–564.

    Speckens, A. E., Ehlers, A., Hackmann, A. & Clark, D. M. (2006).
    Changes in intrusive memories associated with imaginal reliving in
    posttraumatic stress disorder. Journal of Anxiety Disorders 20,
    328–341.

    Early predictors of chronic PTSD 1467

    Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.

    Am J Psychiatry 166:3, March 2009 293

    Reviews and Overviews

    ajp.psychiatryonline.org

    Systematic Review and Meta-Analysis of Multiple-Session
    Early Interventions Following Traumatic Events

    Neil P. Roberts, D.Clin.Psy.

    Neil J. Kitchiner, M.Sc.

    Justin Kenardy, Ph.D.

    Jonathan I. Bisson, D.M.,
    F.R.C.Psych.

    Objective: The authors sought to deter-
    mine the efficacy of multiple-session psy-
    chological interventions to prevent and
    treat traumatic stress symptoms beginning
    within 3 months of a traumatic event.

    Method: Nine computerized databases
    were searched, and manual searches
    were conducted of reference lists of se-
    lected articles as well as two journals. In
    addition, key researchers in the field were
    contacted to determine whether they
    were aware of other relevant studies. The
    reviewers identified randomized con-
    trolled trials of multiple-session psycho-
    logical treatments aimed at preventing or
    reducing traumatic stress symptoms in in-
    dividuals within 3 months of exposure to
    a traumatic event. Details of the studies
    were independently extracted by two re-
    viewers, and outcome data were entered
    into the Review Manager software pack-

    age. Quality assessment was also con-
    ducted by two researchers independently.

    Results: Twenty-five studies examining a
    range of interventions were identified.
    For treatment of individuals exposed to a
    trauma irrespective of their symptoms,
    there was no significant difference be-
    tween any intervention and usual care.
    For treatment of traumatic stress symp-
    toms irrespective of diagnosis, trauma-fo-
    cused cognitive-behavioral therapy (CBT)
    was more effective than waiting list or
    supportive counseling conditions. The dif-
    ference was greatest for treatment of
    acute stress disorder and acute posttrau-
    matic stress disorder.

    Conclusions: Trauma-focused CBT within
    3 months of a traumatic event appears to
    be effective for individuals with traumatic
    stress symptoms, especially those who
    meet the threshold for a clinical diagnosis.

    (Am J Psychiatry 2009; 166:293–301)

    There is now a large body of literature to show that a va-
    riety of traumatic experiences can cause significant psy-
    chological difficulties for large numbers of people (see ref-
    erences 1–6, for example). Many individuals show great
    resilience in the face of such experiences and will manifest
    short-lived or subclinical stress reactions that diminish
    over time (7), and most people recover without medical or
    psychological assistance (8). Nevertheless, a range of psy-
    chological difficulties may develop following trauma in
    some of those who have been exposed, including acute
    stress disorder and posttraumatic stress disorder (PTSD).
    The rate of acute stress disorder has been reported to be
    13% in motor vehicle accident survivors (9) and 19% in
    victims of violent crime (10). Reported rates of acute PTSD
    (defined as PTSD symptoms for less than 3 months) have
    varied across different trauma populations, from 23% in
    motor vehicle accident victims (6) to 47% in rape victims
    (11). Epidemiological research suggests that one-third of
    individuals who develop acute PTSD remain symptomatic
    for 6 years or longer (12). The impact on social, interper-
    sonal, and occupational functioning can be marked and
    enduring for those who develop chronic PTSD (13).

    Over the past three decades, clinicians have been in-
    creasingly involved in attempts to develop interventions
    that might mitigate the effects of trauma and prevent the

    onset of chronic PTSD. Although psychological debriefing

    (also known as critical incident stress debriefing) was a

    widely used intervention for some years, it came under in-

    creasing scrutiny in the 1990s. Systematic reviews (14, 15)

    failed to find evidence for the efficacy of single-session in-

    dividual debriefing, and many experts in the field now

    caution against its use (16, 17). Increasingly the field has

    turned its attention to other models of intervention (13,

    18–21). A common theme has been the suggestion that ef-

    forts should be focused on identifying those most at risk of

    developing ongoing problems in the aftermath of trau-

    matic incidents and directing resources and interventions

    mainly to them.

    Although there is considerable evidence for the efficacy

    of multiple-session trauma-focused psychological inter-

    ventions to treat chronic PTSD (22–24), the early use of

    such interventions has not received the same level of scru-

    tiny. A number of randomized controlled trials have been

    conducted with early interventions, but the issues of how

    effective they are, whom to offer intervention to, the tim-

    ing of intervention, and the mode of intervention remain

    contentious. To help clarify the potential utility of these

    interventions, we performed a systematic review and

    meta-analysis of randomized controlled trials of psycho-

    294 Am J Psychiatry 166:3, March 2009

    EARLY INTERVENTIONS FOLLOWING TRAUMATIC EVENTS

    ajp.psychiatryonline.org

    logical interventions aimed at preventing or treating PTSD
    within 3 months of a traumatic event.

    Method

    Data Sources

    The electronic databases MEDLINE, ClinPSYC, PsychLit, EM-
    BASE, PILOTS, LILACS, PSYNEBS, SocioFile, CINAHL, and the Co-
    chrane Depression, Anxiety, and Neurosis Group Trials Register
    were searched until July 2007 using the Cochrane optimal ran-
    domized controlled trial search strategy combined with the fol-
    lowing keywords: PTSD, trauma, acute stress disorder, acute
    posttraumatic stress disorder, acute post-traumatic stress disor-
    der, early intervention, early psychological intervention, preven-

    tative, prevention, PTSD prevention, crisis, crisis intervention,
    psychological first aid, cognitive, behaviour, behavior, behav-
    ioural, behavioral, cognitive-behavioural, cognitive-behavioral,
    exposure, eye movement desensitization and reprocessing, psy-
    chological, psychotherapy, psychodynamic, stress inoculation,
    relaxation, anxiety management, psychoeducation, collaborative
    care, collaborative intervention, recovery, facilitating recovery,
    critical incident stress debriefing, debriefing, critical incident
    stress management, counseling, counselling, supportive counsel-
    ling, and supportive counseling. Manual searches were under-
    taken of the Journal of Traumatic Stress and the Journal of Con-
    sulting and Clinical Psychology. Reference lists of studies
    identified in the search, related review articles, and management
    guidelines were scrutinized. Internet searches of known web sites
    and discussion forums were conducted, and key researchers in

    TABLE 1. Summary of Meta-Analysis of Results for Interventions for PTSD Symptoms Within 3 Months After a Traumatic Eventa

    Comparison Follow-Up
    Number
    of Trials

    Total
    N

    Interventions beginning within 1 month for all exposed to
    the traumatic event

    Any intervention vs. usual care (PTSD symptoms self-report) Posttreatment (37, 40–43) 5 431
    3–6 months after trauma (41–43) 3 234

    Any intervention vs. usual care (PTSD diagnosis) Posttreatment (38–43) 6 470
    3–6 months after trauma (38, 39, 41, 43) 4 303

    Interventions beginning within 3 months for individuals
    with traumatic stress symptoms

    Structured writing vs. minimal intervention (PTSD symptoms
    self-report)

    Posttreatment (53; Bugg et al., unpublished data)
    3 months after trauma (Bugg et al., unpublished data)

    2
    1

    146
    104

    Structured writing vs. minimal intervention (PTSD diagnosis) Posttreatment (53) 1 42
    Trauma-focused CBT vs. waiting list (PTSD symptoms

    clinician rated)
    Posttreatment (18, 47, 50–52; Öst et al., unpublished data)
    3–5 months after trauma (51, 52)

    6
    2

    423
    138

    9–11 months after trauma (50, 51) 2 73
    12–18 months after trauma (18) 1 152

    Trauma-focused CBT vs. waiting list (PTSD diagnosis) Posttreatment (18, 47, 50–53; Öst et al., unpublished data) 7 515
    3–5 month follow-up (51, 52) 2 141
    9–11 months after trauma (50, 51) 2 54
    12–18 months after trauma (18) 1 116

    Trauma-focused CBT vs. supportive counseling (PTSD
    symptoms self-report)

    Posttreatment (30, 31, 46, 51)
    3–6 months follow-up (30, 31, 46, 51)

    4
    4

    195
    176

    2–4 years after trauma (Clinician Administered PTSD Scale)
    (33, 34)

    2 94

    Trauma-focused CBT vs. supportive counseling (PTSD
    diagnosis)

    Posttreatment (30, 31, 35, 46, 51)
    3–6 months after trauma (30, 31, 35, 46, 51)

    5
    5

    251
    200

    3–4 years after trauma (33, 34) 2 94
    Interventions for individuals with acute stress disorder or

    acute PTSD within 3 monthsd

    Trauma-focused CBT vs. waiting list (PTSD symptoms
    clinician rated)

    Posttreatment (47, 50, 52; Öst et al., unpublished data)
    3–5 months after trauma (52)

    4
    1

    210
    95

    9–11 months after trauma (50) 1 12
    Trauma-focused CBT vs. waiting list (PTSD diagnosis) Posttreatment (47, 50, 52, 53; Öst et al., unpublished data) 5 254

    3–5 months after trauma (52) 1 95
    9–11 months after trauma (50) 1 12

    Trauma-focused CBT vs. supportive counseling (PTSD
    symptoms clinician rated)

    Posttreatment (30, 31, 46)
    3–6 months follow-up (30, 31, 46)

    3
    3

    138
    134

    2–4 years after trauma (Clinician Administered PTSD Scale)
    (33, 34)

    2 94

    Trauma-focused CBT vs. supportive counseling (PTSD
    diagnosis)

    Posttreatment (30, 31, 35, 46)
    3–6 months after trauma (30, 31, 35, 46)

    4
    4

    191
    158

    3–4 years after trauma (33, 34) 2 94
    a PTSD=posttraumatic stress disorder; CBT=cognitive-behavioral therapy.
    b Relative risk of diagnosis of PTSD; a value of 1 indicates that the intervention was the same as the control condition; a value <1 indicates that

    the intervention was better than the control condition; and a value >1 indicates that the control condition was better than the intervention.
    c The standardized mean difference in continuous PTSD symptom score was used when different outcome measures were used in the studies

    included; the weighted mean difference in continuous PTSD symptom score was used when the same outcome measure was used in the
    studies included. A score of 0 indicates that there was no difference between the intervention and the control condition, a score <0 indicates that the intervention was better, and a score >0 indicates that the control condition was better.

    d These analyses are subanalyses of the analyses of interventions beginning within 3 months for individuals with traumatic stress symptoms.
    *p<0.05.

    Am J Psychiatry 166:3, March 2009 295

    ROBERTS, KITCHINER, KENARDY, ET AL.

    ajp.psychiatryonline.org

    the field were contacted to determine whether they were aware of
    other relevant studies.

    Study Selection

    All abstracts were read independently by two of the reviewers
    to determine whether they potentially met the inclusion criteria.
    If either reviewer thought the study potentially met the criteria,
    the full manuscript was obtained and read independently by
    three of the reviewers. To be included, a study had to be a ran-
    domized controlled trial that considered one or more defined
    psychological interventions or treatments (excluding single-ses-
    sion interventions) aimed at preventing or reducing traumatic
    stress symptoms following events that appeared to fulfill DSM-IV
    criterion A1 for PTSD or acute stress disorder in comparison with
    a placebo control, other control (e.g., usual care or waiting list

    control), or alternative psychological treatment condition. All
    studies had to have been completed and analyzed by September
    2007. Presence or absence of symptoms, sample size, language,
    and publication status were not used to determine whether a
    study should be included. The review considered studies involv-
    ing adults only.

    Data Extraction

    A data extraction sheet was designed to record data, which
    were then entered into the Review Manager software package,
    version 4.2 (25). Information extracted included demographic de-
    tails of participants, details of the traumatic event, the random-
    ization process, the interventions used, dropout rates, and out-
    come data. Quality was assessed by rating studies according to
    randomization, allocation concealment, blinding of assessors,
    and intention to treat. Each study was also rated using quality as-
    sessment criteria derived from the literature on key methodolog-
    ical issues pertinent to psychological intervention research on
    traumatic stress (26–29). Data were extracted and quality as-
    sessed by two reviewers independently. Any disagreements were
    discussed with a third reviewer and a consensus achieved.

    Data Synthesis

    Given the differences between studies with respect to partici-
    pants’ symptom severity and the interval between exposure to a
    traumatic event and commencement of the intervention, we sep-
    arated the trials into three groups based on work previously con-
    ducted in this area (16): studies that offered an intervention to
    any individual exposed to a traumatic event irrespective of their
    symptoms with the aim of preventing PTSD; studies providing in-
    terventions begun within 3 months with the aim of preventing
    PTSD or ongoing distress in individuals with traumatic stress
    symptoms; and studies providing interventions begun within 3
    months with the aim of preventing PTSD or ongoing distress in
    individuals with acute stress disorder or acute PTSD.

    In order to combine information from several studies, all inter-
    ventions offered to any individual exposed to a traumatic event
    with the aim of preventing PTSD were considered together. The
    efficacy of trauma-focused cognitive-behavioral therapy (CBT)
    was considered in individuals with traumatic stress symptoms.
    Trauma-focused CBT was defined as any intervention that fo-
    cused on the trauma using exposure to trauma memories and
    trauma reminders with or without cognitive therapy and other
    cognitive-behavioral techniques. The exposure-based therapy
    with anxiety management (30) and exposure-based therapy with
    hypnosis (31) arms in two studies were combined with the expo-
    sure therapy arm to generate a single mean and standard devia-
    tion. The combined results were then compared with the waiting
    list arm to avoid double counting.

    The data were analyzed for summary effects using Review Man-
    ager. Continuous outcomes were analyzed using weighted mean
    differences when all trials measured outcome on the same scale.
    When some trials measured outcomes on different scales, stan-
    dardized mean differences were used, based on the assumption
    that all scales measure the same underlying symptom or condition.
    Relative risk was calculated for categorical outcome measures, and
    95% confidence intervals were computed for all outcomes.

    Available case analysis and intent-to-treat analysis with impu-
    tation using the last-observation-carried-forward method were
    performed when enough information was available. In cases
    where the information presented in the paper was inadequate to
    perform these analyses, further information was requested from
    the lead author.

    Heterogeneity between studies was assessed by observing the
    I2 test of heterogeneity, which measures the percentage of varia-
    tion that is not due to chance (32). An I2 of less than 30% was
    taken to indicate mild heterogeneity, and a fixed-effects model

    Relative
    Riskb 95% CI

    Standardized or
    Weighted Mean

    Difference in
    PTSD Scorec 95% CI

    0.05 –0.26 to 0.36
    0.26 0.00 to 0.52

    0.82 0.56 to 1.20
    0.59 0.27 to 1.25

    –0.28
    1.10

    –1.22 to 0.65
    –3.80 to 6.00

    0.61 0.25 to 1.47
    –0.54
    –0.22

    –0.93 to –0.16*
    –0.56 to 0.11

    –0.85 –2.49 to 0.79
    –6.01 –12.44 to 0.42

    0.72 0.5 to 1.05
    0.64 0.42 to 0.99*
    0.42 0.03 to 5.23
    0.74 0.36 to 1.51

    –0.95
    –0.62

    –1.66 to –0.23*
    –0.94 to –0.31*

    –0.85 –1.29 to –0.40*

    0.56 0.29 to 1.06
    0.37 0.20 to 0.67*

    0.28 0.13 to 0.58*

    –0.85
    –0.88

    –1.35 to –0.34*
    –6.52 to 4.76

    –33.67 –52.77 to –14.57*
    0.54 0.31 to 0.95*
    0.85 0.50 to 1.44
    0.09 0.01 to 1.35

    –20.66
    –0.77

    –28.81 to –12.51*
    –1.14 to –0.40*

    –0.85 –1.29 to –0.40*

    0.44
    0.26

    0.22 to 0.86*
    0.16 to 0.45*

    0.28 0.13 to 0.58*

    296 Am J Psychiatry 166:3, March 2009

    EARLY INTERVENTIONS FOLLOWING TRAUMATIC EVENTS

    ajp.psychiatryonline.org

    was used. When the I2 was 30% or greater, a random-effects
    model was used. A visual inspection of the forest plots was used
    as a test of the robustness of these findings.

    Results

    Two hundred fifty titles and abstracts were identified
    through the search process, and 49 papers were reviewed
    in detail by three of the authors independently to establish
    whether they met the inclusion criteria. Twenty-five stud-
    ies were found to meet the inclusion criteria, and another
    two papers (33, 34) reported long-term follow-up on three
    studies (30, 31, 35). Twenty-four studies were reported in
    English and one (36) in French. A flow diagram of the sys-
    tematic review and a table summarizing the characteris-
    tics of the studies are presented in the data supplement
    that accompanies the online edition of this article.

    Synthesis of Results

    The outcomes for individual studies are indicated in Ta-
    ble S1 in the online data supplement. The postinterven-

    tion and follow-up results of the meta-analyses for com-
    parisons that included more than one study are listed in
    Table 1, and examples of forest plots are presented in Fig-
    ures 1–3. The outcomes reported are rates of PTSD and se-
    verity of PTSD (clinician rated unless unavailable, in
    which case self-report data are used).

    Studies offering intervention to individuals involved
    in a traumatic event irrespective of symptoms.
    Eight studies (36–42, 43) evaluated brief psychosocial in-
    terventions aimed at preventing PTSD in individuals ex-
    posed to a specific traumatic event. All started within 1
    month of the trauma. Meta-analysis of the studies with
    sufficient data available showed no significant differences
    between those who received an intervention and those
    who did not (see Table 1; see also Figure S1 in the online
    data supplement). The only statistically significant differ-
    ences observed for specific interventions were in favor of
    the waiting list control group over adapted critical inci-
    dent stress debriefing for self-reported PTSD symptoms
    immediately after the trauma (41) and for preventive

    FIGURE 1. Forest Plot Comparing Any Multiple-Session Early Intervention and Treatment as Usual for Prevention of PTSD
    in Individuals Exposed to a Traumatic Event, With PTSD Diagnosis as Outcome Measurea

    a Total events: treatment, 36; control, 43. Test for heterogeneity: χ2=7.97, df=5, p=0.16, I2=37.2%. Test for overall effect: z=1.01, p=0.31.

    FIGURE 2. Forest Plot Comparing Trauma-Focused Cognitive-Behavioral Therapy and Waiting List in Individuals Exposed to
    a Traumatic Event Who Have Symptoms of PTSD or Acute Stress Disorder, With PTSD Diagnosis as Outcome Measurea

    a Total events: treatment, 119; control, 142. Test for heterogeneity: χ2=21.01, df=6, p=0.002, I2=71.4%. Test for overall effect: z=1.71, p=0.09.

    Study Treatment Control
    Favors Treatment Favors Control Weight

    (%)
    Relative Risk (Fixed)

    (95% CI)

    Ryding et al. 1998 (39) 2/50 1/49 2.31 1.96 (0.18, 20.92)

    Gidron et al. 2001 (44) 1/8 4/9 8.61 0.28 (0.04, 2.02)

    Zatzick et al. 2001 (43) 2/14 6/15 13.25 0.36 (0.09, 1.48)

    Ryding et al. 2004 (40) 10/82 15/65 38.28 0.53 (0.25, 1.10)

    Gamble et al. 2005 (38) 18/50 16/53 35.53 1.19 (0.69, 2.07)

    Marchand et al. 2006 (41) 3/33 1/42 2.01 3.82 (0.42, 35.04)

    Total 237 233 100.00 0.82 (0.56, 1.20)

    0.1 1.0 10.05.02.00.50.2

    Relative Risk (Fixed)
    (95% CI)

    Study Treatment Control
    Favors Treatment Favors Control Weight

    (%)
    Relative Risk (Fixed)

    (95% CI)

    Bryant et al. 2008 (47) 10/30 23/30 15.82 0.43 (0.25, 0.75)

    Öst (unpublished) 3/23 13/20 7.71 0.20 (0.07, 0.60)

    Ehlers et al. 2003 (50) 1/6 5/6 3.55 0.20 (0.03, 1.24)

    Bisson et al. 2004 (48) 35/76 29/76 19.18 1.21 (0.83, 1.76)

    Foa et al. 2006 (51) 21/31 19/30 19.39 1.07 (0.74, 1.54)

    Sijbrandij et al. 2007 (52) 40/79 43/64 21.05 0.75 (0.57, 0.99)

    van Emmerik et al. 2008 (53) 9/21 10/23 13.30 0.99 (0.50, 1.94)

    Total 266 249 100.00 0.72 (0.50, 1.05)

    0.1 1.0 10.05.02.00.50.2

    Relative Risk (Fixed)
    (95% CI)

    Am J Psychiatry 166:3, March 2009 297

    ROBERTS, KITCHINER, KENARDY, ET AL.

    ajp.psychiatryonline.org

    counseling over monitoring or usual care for clinician-as-
    sessed PTSD symptoms 3 months after trauma (38). Two
    studies (44, 45) evaluated a two-session memory structur-
    ing intervention in individuals who had been involved in a
    motor vehicle accident and had a heart rate >95 bpm in
    the emergency department. No statistically significant dif-
    ferences were found between the interventions and the
    control conditions.

    Studies offering intervention to individuals with
    traumatic stress symptoms within 3 months after a
    traumatic event. Fifteen studies (30, 31, 35, 46–55; A.
    Bugg et al., unpublished 2007 data; L. Öst et al., unpub-
    lished 2004 data) evaluated interventions for individuals
    who had traumatic stress symptoms within 3 months after
    a traumatic event. No statistically significant differences
    were observed between structured writing and minimal
    intervention. Statistically significant differences were ob-
    served in favor of trauma-focused CBT over the waiting list
    condition and supportive counseling at posttreatment as-
    sessment. Follow-up data were incomplete, but statisti-
    cally significant differences were present at several time
    points, particularly over supportive counseling.

    Studies offering intervention to individuals with a di-
    agnosis of acute stress disorder or acute PTSD within
    3 months after a traumatic event. Eleven studies (30,
    31, 35, 46, 47 , 49, 50, 52, 53; Bugg et al., unpublished 2007
    data; Öst et al., unpublished 2004 data) offered interven-
    tions to individuals with a diagnosis of acute stress disorder
    or acute PTSD. The analyses of these studies are subanaly-
    ses of the analyses of symptomatic individuals described
    above, with four studies excluded. Statistically significant
    differences were observed in favor of trauma-focused CBT
    over the waiting list control condition and supportive coun-
    seling. The only evidence to support any other form of
    treatment was for cognitive restructuring, which was signif-
    icantly better than the waiting list condition but less effec-
    tive than trauma-focused CBT in one study (47).

    Methodological Quality of Included Studies

    Only 12 studies adequately described a method of allo-
    cation judged to make no bias possible (31, 37, 38, 43, 46–
    48, 50, 52, 53, 55; Bugg et al., unpublished 2007 data). Re-
    porting of adequate concealment procedures was present
    in only seven studies (31, 37, 43, 47, 48, 50; Bugg et al., un-
    published 2007 data). Adequate blinding of the assessor of
    outcome measures was present in 16 studies (30, 31, 35, 38,
    41, 43, 44–48, 50, 51, 53, 55); Bugg et al., unpublished 2007
    data). Loss to follow-up was fully reported with reasons by
    group in 16 studies (31, 35, 38, 41, 43, 46–54; Bugg et al., un-
    published 2007 data; Öst et al., unpublished 2004 data).

    The overall quality of the studies in relation to the other
    methodological and reporting factors considered was
    variable. Fewer than 10 studies fully reported whether
    training was offered to assessors and how performance,
    supervision, or reliability checks of assessors were per-
    formed (four studies), whether treatment fidelity was
    independently checked and adequate (five studies),
    whether power calculation was reported (four studies),
    whether follow-up extended beyond 6 months (nine stud-
    ies), and whether there were any side effects (one study).

    In order to determine the impact of quality on outcome,
    the three studies included in the analysis of trauma-fo-
    cused CBT versus waiting list for individuals with trau-
    matic stress symptoms (47, 48 , 50) that achieved the high-
    est quality ratings for the four Cochrane quality criteria
    were included in a sensitivity analysis. This resulted in a
    reduction in the magnitude of intervention effect, with su-
    periority over waiting list control only approaching statis-
    tical significance (three studies, N=224, standardized
    mean difference –0.75, 95% CI=–1.53 to 0.02).

    Discussion

    Main Findings

    There was no evidence that a multiple-session interven-
    tion aimed at everyone, irrespective of their symptoms,

    FIGURE 3. Forest Plot Comparing Trauma-Focused Cognitive-Behavioral Therapy and Waiting List in Individuals Exposed to
    a Traumatic Event Who Have Symptoms of PTSD or Acute Stress Disorder, Excluding Those Not Meeting Full Diagnostic Cri-
    teria, With PTSD Diagnosis as Outcome Measurea

    a Total events: treatment, 47; control, 76. Test for heterogeneity: χ2=12.11, df=4, p=0.02, I2=67.0%. Test for overall effect: z=2.15, p=0.03.

    Study Treatment Control
    Favors Treatment Favors Control Weight

    (%)
    Relative Risk (Fixed)

    (95% CI)

    Bryant et al. 2008 (47) 10/30 23/30 25.83 0.43 (0.25, 0.75)

    Öst (unpublished) 3/23 13/20 14.74 0.20 (0.07, 0.60)

    Ehlers et al. 2003 (50) 1/6 5/6 7.43 0.20 (0.03, 1.24)

    Sijbrandij et al. 2007 (52) 24/50 24/45 29.26 0.86 (0.59, 1.28)

    van Emmerik et al. 2008 (53) 9/21 10/23 22.75 0.99 (0.50, 1.94)

    Total 130 124 100.00 0.54 (0.31, 0.95)

    0.1 1.0 10.05.02.00.50.2

    Relative Risk (Fixed)
    (95% CI)

    298 Am J Psychiatry 166:3, March 2009

    EARLY INTERVENTIONS FOLLOWING TRAUMATIC EVENTS

    ajp.psychiatryonline.org

    following a traumatic event was effective. Trauma-focused
    CBT was significantly better than waiting list or usual care
    at reducing traumatic stress symptoms in individuals who
    were symptomatic at entry into the study, but the magni-

    tude of effect varied. The magnitude was largest for indi-
    viduals who were diagnosed with acute stress disorder or
    acute PTSD. Evidence of the benefits of trauma-focused
    CBT for symptomatic individuals who did not meet full di-
    agnostic criteria for these conditions was weak.

    Heterogeneity

    There was evidence of both clinical and statistical het-
    erogeneity in the included studies.

    Although all the trials attempted to reduce traumatic
    stress symptoms, the nature of the interventions was quite
    diverse. This was partially dealt with by separating inter-
    ventions into predetermined groups, although we decided
    to combine the interventions provided to individuals irre-
    spective of their symptoms in order to maximize the infor-
    mation available. There were more studies evaluating
    trauma-focused CBT than other interventions, but the spe-
    cific interventions in the trauma-focused CBT group were
    not identical. All were trauma focused, but some interven-
    tions primarily used a prolonged exposure paradigm (e.g.,
    reference 48), whereas others (e.g., reference 50) primarily
    used cognitive techniques with more limited exposure. It
    was not possible to conduct a formal analysis comparing
    studies with varying amounts of exposure because of lim-
    ited detail regarding the specific amount of exposure deliv-
    ered. However, there did not appear to be a direct relation-
    ship between more exposure and improved outcome or
    vice versa among interventions included in the trauma-fo-
    cused CBT group. This seems at odds with the Institute of
    Medicine’s recent conclusion that exposure therapy is the
    only type of psychological treatment with sufficient evi-
    dence of efficacy in the treatment of PTSD (56), although
    exposure therapy was superior to cognitive restructuring
    with no exposure in the single study included in this review

    that directly compared these two interventions (47).

    In addition, the total number of hours of intervention
    provided varied from around 4 hours to around 16 hours.
    There were also differences in the clinical populations, es-
    pecially with regard to the severity of symptoms at entry to
    the studies. On the basis of previous work on this topic
    (16), we grouped trials in a clinically meaningful manner
    according to the intervention and the clinical populations
    included, but this approach is not empirically based,
    which should be considered when interpreting the results.
    We concluded that the trials grouped together were essen-

    tially trying to measure the same thing and that it was
    worthwhile summarizing their combined results, but the
    variation means that caution should be applied when in-
    terpreting the results (32).

    Methodological Quality

    The overall quality of the studies was varied. Using the
    Cochrane quality criteria, 16 (64%) studies fully reported
    loss to follow-up with reasons, 15 (60%) described using
    appropriately blinded assessors to measure outcome, 12
    (48%) described appropriate randomization with no bias
    possible, and only seven (28%) reported adequate alloca-
    tion concealment. The small sample sizes of most of the
    studies are also an important limitation. However, the in-
    tervention and control groups appeared well matched at
    baseline in most studies, reducing the risk of the re-
    ported unadjusted mean outcomes being influenced by
    baseline differences.

    Several studies, including those that provided more pos-
    itive results, had strong methodological characteristics. A
    meta-analysis of the highest-quality studies resulted in a
    larger effect size (0.75) for the efficacy of trauma-focused
    CBT versus waiting list control than the meta-analysis of
    all studies irrespective of quality (0.54), but the former
    meta-analysis just failed to reach statistical significance.
    This is probably a power issue and, contrary to previous
    research (57), does not suggest that poorer-quality studies
    falsely elevated the apparent efficacy of the intervention.

    The choice of control condition is particularly impor-
    tant in early intervention research, where a reduction in
    symptoms over the duration of the trial would be expected
    given the natural course of traumatic stress reactions (58).
    The development of a psychological treatment placebo is
    very difficult, if not impossible, as is blinding of partici-
    pants and therapists. Some of the waiting list and usual
    care groups may have received some form of intervention
    by virtue of contact through symptom monitoring, but
    this was not properly evaluated, and it is not possible to
    determine what impact this might have had on outcomes.

    Only one study (47) reported adverse effects, and it is
    unclear whether any occurred in other studies. The drop-
    out rates were mostly no higher in the intervention groups
    than in the control groups across the studies reviewed,
    which suggests that the interventions did not cause major
    adverse effects. However, the absence of tolerability as-
    sessment is a key shortcoming in the trials identified and
    one that has previously been noted in psychological treat-
    ment studies of chronic PTSD (22).

    Implications for Practice

    The results suggest that no psychological intervention
    can be recommended for routine use following traumatic
    events. This is consistent with the results of single-session
    interventions, although, in contrast to them (14), with the
    possible exception of adapted critical incident stress de-
    briefing, no evidence was found of any harm occurring as
    a result of an intervention. Trauma-focused CBT was the
    only early intervention with convincing evidence of effi-
    cacy in reducing and preventing traumatic stress symp-
    toms, but only for symptomatic individuals and particu-
    larly for those who met the diagnostic criteria for acute

    Am J Psychiatry 166:3, March 2009 299

    ROBERTS, KITCHINER, KENARDY, ET AL.

    ajp.psychiatryonline.org

    stress disorder or acute PTSD. The less convincing evi-
    dence in favor of trauma-focused CBT for all symptomatic
    individuals raises some interesting clinical implications.
    Positive outcomes in the meta-analysis for all symptom-
    atic individuals appear to have been bolstered by the out-
    comes from studies focusing specifically on individuals
    meeting all diagnostic criteria for acute stress disorder and
    acute PTSD. This suggests that the presence of a specific
    diagnosis may be the most important predictor of who will
    benefit from trauma-focused CBT. However, when plan-
    ning how best to detect such individuals, it is important to
    heed the research suggesting that merely screening for
    acute stress disorder is problematic as it misses many in-
    dividuals who go on to develop PTSD (59).

    While the majority of symptomatic individuals are likely
    to gain some benefit from trauma-focused CBT 1 to 3
    months after a traumatic event, the magnitude of this ben-
    efit may not be very large. Whether the magnitude of im-
    provement is likely to be significant enough to justify the
    routine provision of trauma-focused CBT to all symptom-
    atic individuals is open to debate. The evidence suggests
    that trauma-focused CBT should be offered to all who suf-
    fer from acute stress disorder or acute PTSD and that lim-
    iting it to this group can be justified, particularly when re-
    sources are limited. The results for non-trauma-focused
    CBT interventions were disappointing, but it remains pos-
    sible that elements from them are effective, particularly if
    used with more symptomatic individuals. For example,
    behavioral reactivation (54) has clearly not yet been evalu-
    ated with an adequately powered trial and would benefit
    from further evaluation.

    The results of this review support calls that have been
    made for a stepped or stratified care system whereby those
    with the most severe symptoms are offered more complex
    interventions (18). The fact that trauma-focused CBT ap-
    pears to be an effective treatment suggests that more work
    should be done to determine whether it could be delivered
    as part of a screening program after major traumatic events.

    Implications for Research

    Further well-designed randomized controlled trials of
    trauma-focused CBT starting within the first 3 months af-
    ter traumatic events with longer follow-up periods are
    needed. Given the modest overall effects of trauma-fo-
    cused CBT, the development and trialing of other psycho-
    logical treatments are important. The finding that expo-
    sure therapy was superior to cognitive restructuring in one
    study requires replication, and the comparison of treat-
    ments with more or less exposure should be pursued in
    the future.

    Most of the studies included in this review attempted to
    evaluate individual psychological therapy. Given the im-
    portant role of social support as a predictor of outcome
    (60, 61), it would be of interest to examine interventions
    aimed at couples and families to improve familial re-
    sponse. It would also be of interest to evaluate forms of

    community intervention and interventions aimed at im-
    proving coping skills and enhancing positive and helpful
    behaviors (J.I. Ruzek, unpublished 2007 paper). Future re-
    search should also consider adverse events and tolerabil-
    ity of treatment, carefully control for additional interven-
    tion, and explore the optimal time to intervention, how
    long treatment should last, and whether other techniques
    can be incorporated into existing treatments to improve
    their efficacy.

    Received April 25, 2008; revisions received July 18 and Oct. 1, 2008;
    accepted Oct. 27, 2008 (doi: 10.1176/appi.ajp.2008.08040590). From
    Cardiff and Vale NHS Trust, Wales; University of Queensland, Austra-
    lia; and Cardiff University, Wales. Address correspondence and reprint
    requests to Dr. Bisson, Cardiff University, Monmouth House, Univer-
    sity Hospital of Wales, Heath Park, Cardiff, CF14 4XW, Wales, UK;
    bissonji@cf.ac.uk (e-mail).

    All authors report no competing interests.
    The authors thank the authors of studies in the review for providing

    unpublished data, Helen Davies for administrative support, and Hugh
    McGuire for help with searches and with translation. Jonathan Bisson
    had full access to all of the data in the study and takes responsibility
    for the integrity of the data and the accuracy of the data analysis.

    References

    1. Goenjian A: A mental health relief programme in Armenia af-
    ter the 1988 earthquake: implementation and clinical obser-
    vations. Br J Psychiatry 1993; 163:230–239

    2. Green BL, Grace MC, Lindy JD, Gleser GC, Leonard AC, Crum-
    mier TL: Buffalo Creek survivors in the second decade: com-
    parison with unexposed and nonlitigant groups. J Appl Soc Psy-
    chol 1990; 20:1033–1050

    3. Kulka RA, Schlenger WE, Fairbank JA, Jordan BK, Hough RL,
    Marmar CR, Weiss DS: Trauma and the Vietnam War Genera-
    tion: Report of Findings From the Vietnam Veterans Study.
    New York, Bruner/Mazel, 1990

    4. Kilpatrick DG, Saunders BE, Veronen LJ, Best CL, Von JM: Crimi-
    nal victimization: lifetime prevalence reporting to police, and
    psychological impact. Crime Delinq 1987; 33:479–489

    5. North CS, Smith EM, Spitznagel EL: Postraumatic stress disorder
    in survivors of a mass shooting. Am J Psychiatry 1994; 151:82–
    88

    6. Ehlers A, Mayou RA, Bryant B: Psychological predictors of
    chronic posttraumatic stress disorder after motor vehicle acci-
    dents. J Abnorm Psychol 1998; 107:508–519

    7. Bonanno GA: Loss, trauma, and human resilience: have we un-
    derestimated the human capacity to thrive after extremely
    aversive events? Am Psychol 2004; 59:20–28

    8. McNally RJ, Bryant RA, Ehlers A: Does early psychological inter-
    vention promote recovery from posttraumatic stress disorder?
    Psychological Science in the Public Interest 2003; 4:45–79

    9. Harvey AG, Bryant RA: The relationship between acute stress
    disorder and posttraumatic stress disorder: a prospective eval-
    uation of motor vehicle accident survivors. J Consult Clin Psy-
    chol 1998; 66:507–512

    10. Brewin CR, Andrews B, Rose S, Kirk M: Acute stress disorder
    and posttraumatic stress disorder in victims of violent crime.
    Am J Psychiatry 1999; 156:360–366

    11. Rothbaum BO, Foa EB, Riggs DS, Murdock T, Walsh W: A pro-
    spective examination of post-traumatic stress disorder in rape
    victims. J Trauma Stress 1992; 5:455–475

    12. Kessler R, Sonnega A, Bromet E, Hughes M, Nelson CB: Post-
    traumatic stress disorder in the National Comorbidity Survey.
    Arch Gen Psychiatry 1995; 52:1048–1060

    300 Am J Psychiatry 166:3, March 2009

    EARLY INTERVENTIONS FOLLOWING TRAUMATIC EVENTS

    ajp.psychiatryonline.org

    13. Litz BT, Gray MJ: Early intervention for trauma in adults: a
    framework for first aid and secondary prevention, in Early In-
    tervention for Trauma and Traumatic Loss. Edited by Litz BT.
    New York, Guilford, 2004

    14. Rose S, Bisson J, Churchill R, Wessely S: Psychological debriefing
    for preventing post traumatic stress disorder (PTSD). Cochrane
    Database Syst Rev 2005; issue 3

    15. van Emmerik AA, Kamphuis JH, Hulsbosch AM, Emmelkamp
    PM: Single session debriefing after psychological trauma: a
    meta-analysis. Lancet 2002; 360:766–771

    16. National Collaborating Centre for Mental Health: Post-Trau-
    matic Stress Disorder: The Management of PTSD in Adults and
    Children in Primary and Secondary Care. London, Gaskell,
    2005

    17. Australian Centre for Posttraumatic Mental Health: Australian
    Guidelines for the Treatment of Adults With Acute Stress Disor-
    der and Posttraumatic Stress Disorder. Melbourne, 2007

    18. Bisson JI, Roberts N, Macho G: Service innovations: the Cardiff
    Traumatic Stress Initiative: an evidence-based approach to
    early psychological intervention following traumatic events.
    Psychiatr Bull 2003; 27:145–147

    19. Ehlers A, Clark DM: Early psychological interventions for adult
    survivors of trauma: a review. Biol Psychiatry 2003; 53:817–
    826

    20. Litz B, Gray M, Bryant R, Adler A: Early intervention for trauma:
    current status and future directions. Clin Psychol 2002; 9:112–
    134

    21. Gray MJ, Litz BT: Behavioral interventions for recent trauma.
    Behav Modif 2005; 29:189–215

    22. Bisson JI, Ehlers A, Matthews R, Pilling S, Richards D, Turner S:
    Psychological treatments for chronic post-traumatic stress dis-
    order: systematic review and meta-analysis. Br J Psychiatry
    2007; 190:97–104

    23. Bradley R, Greene J, Russ E, Dutra L, Westen D: A multidimen-
    sional meta-analysis of psychotherapy for PTSD. Am J Psychia-
    try 2005; 162:214–227

    24. Bisson J, Andrew M: Psychological treatment of post-traumatic
    stress disorder. Cochrane Database Syst Rev 2007; 3:CD003388

    25. Cochrane Collaboration: Review Manager (RevMan) version 4.2
    for Windows. Oxford, Cochrane Collaboration, 2003

    26. Moncrieff J, Churchill R, Drummond C: Development of a qual-
    ity assessment for trials of treatment for depression and neuro-
    sis. Int J Methods Psychiatr Res 2001; 10:126–133

    27. Foa EB, Meadows EA: Psychosocial treatments for posttrau-
    matic stress disorder: a critical review. Annu Rev Psychol 1997;
    48:449–480

    28. Hertlein KM, Ricci RJ: A systematic research synthesis of EMDR
    studies: implementation of the platinum standard. Trauma Vi-
    olence Abuse 2004; 5:285–300

    29. Kenardy J, Carr V: Imbalance in the debriefing debate: what we
    don’t know outweighs what we do. Bulletin of the Australian
    Psychological Society 1996; 17:4–6

    30. Bryant RA, Sackville T, Dang ST, Moulds M, Guthrie R: Treating
    acute stress disorder: an evaluation of cognitive behavior ther-
    apy and supportive counseling techniques. Am J Psychiatry
    1999; 156:1780–1786

    31. Bryant RA, Moulds ML, Guthrie RM, Nixon RDV: The additive
    benefits of hypnosis and cognitive-behavioral therapy in treat-
    ing acute stress disorder. J Consult Clin Psychol 2005; 73:334–
    340

    32. Fletcher J: What is heterogeneity and is it important? Br Med J
    2007; 334:94–96

    33. Bryant RA, Moulds ML, Nixon RVD: Cognitive behaviour ther-
    apy of acute stress disorder: a four-year follow-up. Behav Res
    Ther 2003; 41:489–494

    34. Bryant RA, Moulds ML, Nixon RDV, Mastrodomenico J, Felming-
    ham K, Hopwood S: Hypnotherapy and cognitive behaviour

    therapy of acute stress disorder: a 3-year follow-up. Behav Res
    Ther 2006; 44:1331–1335

    35. Bryant RA, Harvey AG, Dang ST, Sackville T, Basten C: Treatment
    of acute stress disorder: a comparison of cognitive-behavioral
    therapy and supportive counseling. J Consult Clin Psychol
    1998; 66:862–866

    36. André C, Lelord F, Légeron P, Reignier A, Delattre A: [Effective-
    ness of early intervention on 132 bus drivers victims of aggres-
    sions: a controlled trial]. L’Encephale 1997; 23:65–71 (French)

    37. Kazak AE, Simms S, Alderfer MA, Rourke MT, Crump T, McClure
    K, Jones P, Rodriguez A, Boeving A, Hwang W, Reilly A: Feasibil-
    ity and preliminary outcomes from a pilot study of a brief psy-
    chological intervention for families of children newly diag-
    nosed with cancer. J Pediatr Psychol 2005; 30:644–655

    38. Gamble J, Creedy D, Moyle W, Webster J, McAllister M, Dickson
    P: Effectiveness of a counseling intervention after a traumatic
    childbirth: a randomized controlled trial. Birth 2005; 32:11–19

    39. Ryding E, Wijma K, Wijma B: Postpartum counselling after an
    emergency caesarean. Clin Psychol Psychother 1998; 5:231–
    237

    40. Ryding El, Wiren E, Johansson G, Ceder B, Dahlstrom A: Group
    counseling for mothers after emergency cesarean section: a
    randomized controlled trial of intervention. Birth 2004; 31:
    247–253

    41. Marchand A, Guay S, Boyer R, Iucci S, Martin A, St-Hilaire M: A
    randomized controlled trial of an adapted form of individual
    critical incident stress debriefing for victims of an armed rob-
    bery. Brief Treat Crisis Interv 2006; 6:122–129

    42. Brom D, Kleber RJ, Hofman MC: Victims of traffic accidents: in-
    cidence and prevention of post-traumatic stress disorder. J Clin
    Psychol 1993; 49:131–140

    43. Zatzick D, Roy-Byrne P, Russo JE, Rivara FP, Koike A, Jurkovich
    GJ, Katon W: Collaborative interventions for physically injured
    trauma survivors: a pilot randomized effectiveness trial. Gen
    Hosp Psychiatry 2001; 23:114–123

    44. Gidron Y, Gal R, Freedman S, Twiser I, Lauden A, Snir Y, Ben-
    jamin J: Translating research findings to PTSD prevention: re-
    sults of a randomized-controlled pilot study. J Trauma Stress
    2001; 14:773–780

    45. Gidron Y, Gal R, Givati G, Lauden A, Snir Y, Binjamin J: Interac-
    tive effects of memory structuring and gender in preventing
    posttraumatic stress symptoms. J Nerv Ment Dis 2007; 195:1–4

    46. Bryant RA, Moulds M, Guthrie R, Nixon RDV: Treating acute
    stress disorder following mild traumatic brain injury (letter).
    Am J Psychiatry 2003; 160:585–587

    47. Bryant RA, Mastrodomenico J, Felmingham KL, Hopwood S,
    Kenny L, Kandris E, Cahill C, Creamer M: Treatment of acute
    stress disorder: a randomized controlled trial. Arch Gen Psychi-
    atry 2008; 65:659–667

    48. Bisson JI, Shepherd JP, Joy D, Probert R, Newcombe RG: Early
    cognitive-behavioural therapy for post-traumatic stress symp-
    toms after physical injury. Br J Psychiatry 2004; 184:63–69

    49. Echeburua E, de Corral P, Sarasua B, Zubizarreta I: Treatment
    of acute posttraumatic stress disorder in rape victims: an ex-
    perimental study. J Anxiety Disord 1996; 10:185–199

    50. Ehlers A, Clark D, Hackmann A, McManus F, Fennell M, Herbert
    C, Mayou R: A randomized controlled trial of cognitive therapy,
    a self-help booklet, and repeated assessments as early inter-
    ventions for posttraumatic stress disorder. Arch Gen Psychiatry
    2003; 60:1024–1032

    51. Foa EB, Zoellner LA, Feeny NC: An evaluation of three brief pro-
    grams for facilitating recovery after assault. J Trauma Stress
    2006; 19:29–43

    52. Sijbrandij M, Olff M, Reitsma JB, Carlier IVE, de Vries MH, Ger-
    sons BPR: Treatment of acute posttraumatic stress disorder
    with brief cognitive behavioral therapy: a randomized con-
    trolled trial. Am J Psychiatry 2007; 164:82–90

    Am J Psychiatry 166:3, March 2009 301

    ROBERTS, KITCHINER, KENARDY, ET AL.

    ajp.psychiatryonline.org

    53. van Emmerik AA, Kamphuis JH, Emmelkamp PM: Treating
    acute stress disorder and posttraumatic stress disorder with
    cognitive behavioral therapy or structured writing therapy: a
    randomized controlled trial. Psychother Psychosom 2008; 77:
    93–100

    54. Wagner AW, Zatzick DF, Ghesquiere A, Jurkovich GJ: Behavioral
    activation as an early intervention for posttraumatic stress dis-
    order and depression among physically injured trauma survi-
    vors. Cogn Behav Pract 2007; 4:341–349

    55. Zatzick D, Roy-Byrne P, Russo J, Rivara F, Droesch R, Wagner A,
    Dunn C, Jurkovich G, Uehara E, Katon W: A randomized effec-
    tiveness trial of stepped collaborative care for acutely injured
    trauma survivors. Arch Gen Psychiatry 2004; 61:498–506

    56. Committee on Treatment of Posttraumatic Stress Disorder, In-
    stitute of Medicine of the National Academies: Treatment of
    Posttraumatic Stress Disorder: An Assessment of the Evidence.
    Washington, DC, National Academies Press, 2008

    57. Moher D, Pham B, Jones A, Cook DJ, Jadad AR, Moher M, Tug-
    well P, Klassen TP: Does quality of reports of randomised trials
    affect estimates of intervention efficacy in meta-analyses? Lan-
    cet 1998; 352:609–613

    58. Galea S, Vlahov D, Resnick H, Ahern J, Susser E, Gold J, Bucuv-
    alas M, Kilpatrick D: Trends of probable post-traumatic stress
    disorder in New York City after the September 11 terrorist at-
    tacks. Am J Epidemiol 2003; 158:514–524

    59. Bryant R: Acute stress disorder. Psychiatry 2006; 5:238–239
    60. Brewin CR, Andrew B, Valentine JD: Meta-analysis of risk fac-

    tors for posttraumatic stress disorder in trauma-exposed
    adults. J Consult Clin Psychol 2000; 68:748–766

    61. Ozer EJ, Best SR, Lipsey TL, Weiss DS: Predictors of posttrau-
    matic stress disorder and symptoms in adults: a meta-analysis.
    Psychol Bull 2003; 129:52–73

    HELPFUL TUTORIAL ON WAY TO WRITE THIS ASSIGNMENT.

    Read scenario a few times, and highlight points.

    Part A –

    Structure of Part A. Essay form – nothing tricky about this.

    Could make some assumptions in this part.

    Can be a bit flexible in what is happening in scenario.

    Do not spend too much time repeating as only 1500 words. Do not rewrite the scenario – assume the markers know this.

    Client is driving the treatment. Is a good way to write assignment that way.

    You can’t prescribe medication.

    This assessment is not about physical health.

    Planting a seed for community services.

    Paragraph format – an essay.

    Outline of additional information.

    B – 500 words.  

    In letter – pay attention to Mark’s past trauma.

    Write letter. Summarise findings – and make some suggestions.

    Put citations in letter – need for assessment (would not normally do in a letter).

    Letter – reference intervention (treatment suggestions).

    Looking at follow up care – not all provided by GP.

    Mark will read what you have written to GP.

    In letter to GP- could say have conducted risk assessment so he could be discharged as safe.

    Outline things. He is safe. Recommendations to GP.

    Look at what is in your local area and could refer some of this in GP letter.    Then some online stuff is available too.

    Calculate the price of your order

    550 words
    We'll send you the first draft for approval by September 11, 2018 at 10:52 AM
    Total price:
    $26
    The price is based on these factors:
    Academic level
    Number of pages
    Urgency
    Basic features
    • Free title page and bibliography
    • Unlimited revisions
    • Plagiarism-free guarantee
    • Money-back guarantee
    • 24/7 support
    On-demand options
    • Writer’s samples
    • Part-by-part delivery
    • Overnight delivery
    • Copies of used sources
    • Expert Proofreading
    Paper format
    • 275 words per page
    • 12 pt Arial/Times New Roman
    • Double line spacing
    • Any citation style (APA, MLA, Chicago/Turabian, Harvard)

    Our guarantees

    Delivering a high-quality product at a reasonable price is not enough anymore.
    That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.

    Money-back guarantee

    You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.

    Read more

    Zero-plagiarism guarantee

    Each paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.

    Read more

    Free-revision policy

    Thanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.

    Read more

    Confidentiality Guarantee

    Your email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.

    Read more

    Fair-cooperation guarantee

    By sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.

    Read more

    24/7 Support

    Our specialists are always online to help you! We are available 24/7 via live chat, WhatsApp, and phone to answer questions, correct mistakes, or just address your academic fears.

    See our T&Cs
    Live Chat+1(978) 822-0999EmailWhatsApp

    Order your essay today and save 30% with the discount code ESSAYHELP